Effects of HIIT on Plasma Volume and Aerobic Capacity in Obese Adolescent Girls

NCT07046520 | Completed DMC

• 1 other identifier: HIIT-ADO-2025-TN
• Study Type: interventional
• Target: 28
• Locations: 1 country
• Sites: 1

Brief Summary

The goal of this clinical trial is to investigate the impact of high-intensity interval training (HIIT) program on body composition, hematological parameters, plasma volume variations (PVV), muscle damage markers, and aerobic capacity in overweight/obese adolescent girls. The main question it aims to answer is: Does HIIT reduce risk factors associated obesity diseases by modulating body composition and hematological parameters and increasing PVV? Researchers will compare HIIT (designed to the experimental group) to nontraining intervention (designed to the control group) to see if the training program works to enhance physiological health and reduce risk factors associated with obesity in this population. Participants in the experimental (HIIT group) group will: underwent HIIT at 90-105% maximal aerobic speed (MAS), three times a week. Participants in control group will : not perform any physical training and maintain their usual daily activities.

Conditions

Hematologic Test

Keywords

Obesity; High-Intensity Interval Training; Adolescent Girls; Creatine Kinase; Plasma Volume Variation; Aerobic Capacity; Erythrocytes

Outcome Measures

Primary Outcomes (17)

• Erythrocytes

  Blood levels of erythrocytes were collected from a blood sample (5 ml) and analyzed using a multichannel automated hematology analyzer (XN450; Sysmex, Norderstedt, Germany).

  At baseline and at week 11 (end of intervention period).

• Hemoglobin

  Blood levels of hemoglobin were collected from a blood sample (5 ml) and analyzed using a multichannel automated hematology analyzer (XN450; Sysmex, Norderstedt, Germany).

  At baseline and at week 11 (end of intervention period).

• Hematocrit

  Blood levels of hematocrit were collected from a blood sample (5 ml) and analyzed using a multichannel automated hematology analyzer (XN450; Sysmex, Norderstedt, Germany).

  At baseline and at week 11 (end of intervention period).

• Mean corpuscular volume

  Blood levels of mean corpuscular volume were collected from a blood sample (5 ml) and analyzed using a multichannel automated hematology analyzer (XN450; Sysmex, Norderstedt, Germany).

  At baseline and at week 11 (end of intervention period).

• Mean corpuscular hemoglobin content

  Blood levels of mean corpuscular hemoglobin content were collected from a blood sample (5 ml) and analyzed using a multichannel automated hematology analyzer (XN450; Sysmex, Norderstedt, Germany).

  At baseline and at week 11 (end of intervention period).

• Mean hemoglobin concentration

  Blood levels of mean hemoglobin concentration were collected from a blood sample (5 ml) and analyzed using a multichannel automated hematology analyzer (XN450; Sysmex, Norderstedt, Germany).

  At baseline and at week 11 (end of intervention period)

• Creatine kinase

  Serum concentrations of creatine kinase are measured from a blood sample (5 ml) using a Beckman Coulter AU480 Chemistry Analyzer (France).

  At baseline and at week 11 (end of intervention period).

• Lactate dehydrogenase

  Serum concentrations of lactate dehydrogenase are measured from a blood sample (5 ml) using a Beckman Coulter AU480 Chemistry Analyzer (France).

  At baseline and at week 11 (end of intervention period).

• Plasma volume variations

  Plasma volume variations (PVV) was calculated based on measured hematocrit (Ht) and hemoglobin (Hb) values according to the method developed by Costill and Fink (1974). %PVV=100 ×\[(HbA/HbB) ×(100 - HtB) / (100 - HtA)\] - 1, where 0 is the value measured before training program and 1 is the value measured after training program.

  Plasma volume variations (PVV) was calculated at week 11 (end of intervention period) under two conditions (High intensity interval training and No training intervention [control group] ).

• Body weight

  Body weight (kg) was recorded , with barefoot and lightly dressed subjects, using an electronic scale (Tanita BC-533, Tokyo, Japan).

  At baseline and at week 11 (end of intervention period).

• Height

  Height (m) was determined using a standard stadiometer (Holtain Ltd., UK).

  At baseline

• Body fat

  Body fat (%) was recorded using an electronic scale (Tanita BC-533, Tokyo, Japan).

  At baseline and at week 11 (end of intervention period)

• Body mass index

  Body mass index (kg/m²) was calculated as weight (kg) divided by height squared (m²).

  At baseline and at week 11 (end of intervention period).

• Waist circumference

  Waist circumference (cm) was measured to the nearest 0.1 cm using a non-deformable anthropometric tape, placed horizontally at the midpoint between the inferior margin of the last palpable rib and the superior border of the iliac crest, with the participant standing upright and breathing normally.

  At baseline and at week 11 (end of intervention period).

• Maximal aerobic speed

  Maximal aerobic speed was determined using the Vameval test (Cazorla,1990).

  At baseline and at week 11 (end of intervention period).

• Maximal oxygen consumption (VO2max)

  Maximal oxygen consumption (VO2max) were determined using the Vameval test (Cazorla,1990).

  At baseline and at week 11 (end of intervention period).

• Maximal heart rate (HRmax)

  Maximal heart rate maximum (HRmax) was recorded at the final stage of the Vameval test (Cazorla,1990) using a Polar heart rate monitor (Polar S810, Kempele, Finland).

  At baseline and at week 11 (end of intervention period).

Study Arms (2)

High-intensity interval training group

EXPERIMENTAL

High-intensity interval training group performed four series of 15-second runs at 90-105% of the maximal aerobic speed (MAS) interspersed with 15 seconds of active recovery at 5O% MAS.

Behavioral: High intensity interval training

Control group

NO INTERVENTION

No training intervention was intended for the control group.

Interventions

High intensity interval training BEHAVIORAL

HIIT protocol was performed three times per week over a 10-week period, totaling 30 sessions. HIIT sessions included: (1) a standardized warm-up of five minutes of continuous jogging at 50%MAS, followed by five minutes of dynamic mobility and activation drills such as high-knee skips, high-knee running, and butt kicks. (2) a HIIT training session, consisted of four series of 15-second runs at 90-105% of the maximal aerobic speed (MAS) interspersed with 15 seconds of active recovery and (3) a cool-down of five minutes of static stretching.

High-intensity interval training group

Eligibility Criteria

• Age: 15 Years - 17 Years
• Gender Eligibility Details: Female
• Healthy Volunteers: No
• Age Groups: Child (0-17)

You may qualify if:

• Females.
• Aged between 15 and 17 years.
• BMI greater than or equal to the 95th percentile for their age.

You may not qualify if:

• Pharmacological treatments or medical conditions that could interact with the protocol (e.g.,hypertension, diabetes, cardiac, orthopedic, neuromuscular, or neurological disorders).
• Hormone replacement therapy or therapies for obesity.
• Irregular menstrual cycle.
• Restrictive diet or use of dietary supplements or engagement in any structured physical training either currently or during the previous three months.
• Withdrawal of consent.
• Non-compliance with study procedures.
• Insufficient data for analysis.

Sponsors & Collaborators

• High Institute of Sports and Physical Education of Kef: lead

Study Sites (1)

High Institute of Sports and Physical Education of Kef

El Kef, Boulifa, 7100, Tunisia

Location

MeSH Terms

Conditions

Obesity

Interventions

High-Intensity Interval Training

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Wissal abassi, Dr

  Research Unit "Sport Sciences, Health and Movement"(UR22JS01) High Institute of Sport and Physical Education of Kef, University of Jendouba, 7100 Kef, Tunisia.

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: PARTICIPANT
• Purpose: PREVENTION
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: June 23, 2025
• First Posted: July 1, 2025
• Study Start: December 9, 2024
• Primary Completion: February 28, 2025
• Study Completion: February 28, 2025
• Last Updated: July 4, 2025

Record last verified: 2024-12

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)