NCT07017478

Brief Summary

This study will examine the effect of a low dose of the 5HT2A agonist LSD (26 µg), compared to placebo, on acute and protracted mood states in individuals with depression. The investigators will assess the relationship between mood-related symptoms and EEG as a neurophysiological marker.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
48

participants targeted

Target at P50-P75 for early_phase_1

Timeline
1mo left

Started Jun 2025

Shorter than P25 for early_phase_1

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress93%
Jun 2025Jun 2026

First Submitted

Initial submission to the registry

April 22, 2025

Completed
2 months until next milestone

Study Start

First participant enrolled

June 9, 2025

Completed
3 days until next milestone

First Posted

Study publicly available on registry

June 12, 2025

Completed
12 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

June 1, 2026

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2026

Last Updated

July 3, 2025

Status Verified

July 1, 2025

Enrollment Period

12 months

First QC Date

April 22, 2025

Last Update Submit

July 1, 2025

Conditions

Outcome Measures

Primary Outcomes (6)

  • Acute Mood Measures

    Acute (Profile of Mood States scale ratings from 0-60).

    Peak change (~2 hours post-drug) from pre-drug baseline

  • Acute Mood Measures

    Acute (Visual Analog Scale questionnaires, scale ratings from 0-100).

    Peak change (~2 hours post-drug) from pre-drug baseline

  • Delayed Mood Measures

    Delayed depressed mood measures (Beck Depression Inventory \[score range: 0-63, higher scores greater symptoms severity\] and Inventory of depression and anxiety symptoms \[score range: 0-48, higher scores increased severity\])

    Change from baseline (orientation) to 48-hour followup

  • Effort expenditure for reward (EEFRT) Behavioral Task Performance

    Effort Expenditure for Reward Task

    2 hours post-drug administration

  • Sleep Quality

    Electroencephalogram (EEG) power to assess time spent in Rapid Eye Movemeent \[REM\] and slow wave sleep

    Change from baseline (pre-drug) to Night 0, 1, and 2 post-drug

  • Sleep Quality

    subjective measures of sleep function (Karolinska sleep log)

    Change from baseline (pre-drug) to Night 0, 1, and 2 post-drug

Secondary Outcomes (4)

  • Self-reported feelings of connection using Likert scale conversation questionnaires

    Collected at the end of the session, 270 minutes post-drug administration

  • Natural Language Processing using large language model

    during 45 minute conversation, occurring 150 min after drug administration

  • Facial expression analysis using HUMEAI software for positive and negative affect

    during 45 minute conversation, occurring 150 min after drug administration

  • Self-Esteem implicit association test

    2 hours post drug

Study Arms (2)

placebo

PLACEBO COMPARATOR

distilled water (0.26 mL)

Drug: Placebo

LSD (26 micrograms)

EXPERIMENTAL

LSD tartrate in tasteless solution (0.26 mL)

Drug: LSD

Interventions

LSDDRUG

The serotonin 2A receptor agonist LSD

LSD (26 micrograms)

Distilled water

placebo

Eligibility Criteria

Age18 Years - 40 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • English Fluency
  • high school education or higher
  • BMI between 19-30 kg/m2

You may not qualify if:

  • individuals with a medical condition contraindicating study participation as determined by the study physician (e.g., liver disease, abnormal EKG, liver or cardiovascular disease)
  • high blood pressure (\>140/90)
  • current suicidal ideation or suicide attempt in past 12 months
  • past year severe substance use disorder
  • personal or first-degree relative with history of psychosis
  • currently taking any psychiatric medication (for conventional antidepressants must be off for ≥ 2 weeks)
  • active panic disorder
  • severe obsessive-compulsive disorder
  • severe post-traumatic stress disorder
  • women who are pregnant or planning to become pregnant

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University of Chicago

Chicago, Illinois, 60637, United States

RECRUITING

Related Publications (10)

  • Duan W, Cao D, Wang S, Cheng J. Serotonin 2A Receptor (5-HT2AR) Agonists: Psychedelics and Non-Hallucinogenic Analogues as Emerging Antidepressants. Chem Rev. 2024 Jan 10;124(1):124-163. doi: 10.1021/acs.chemrev.3c00375. Epub 2023 Nov 30.

    PMID: 38033123BACKGROUND
  • Husain MI, Ledwos N, Fellows E, Baer J, Rosenblat JD, Blumberger DM, Mulsant BH, Castle DJ. Serotonergic psychedelics for depression: What do we know about neurobiological mechanisms of action? Front Psychiatry. 2023 Feb 10;13:1076459. doi: 10.3389/fpsyt.2022.1076459. eCollection 2022.

    PMID: 36844032BACKGROUND
  • Ling S, Ceban F, Lui LMW, Lee Y, Teopiz KM, Rodrigues NB, Lipsitz O, Gill H, Subramaniapillai M, Mansur RB, Lin K, Ho R, Rosenblat JD, Castle D, McIntyre RS. Molecular Mechanisms of Psilocybin and Implications for the Treatment of Depression. CNS Drugs. 2022 Jan;36(1):17-30. doi: 10.1007/s40263-021-00877-y. Epub 2021 Nov 17.

    PMID: 34791625BACKGROUND
  • Murphy RJ, Muthukumaraswamy S, de Wit H. Microdosing Psychedelics: Current Evidence From Controlled Studies. Biol Psychiatry Cogn Neurosci Neuroimaging. 2024 May;9(5):500-511. doi: 10.1016/j.bpsc.2024.01.002. Epub 2024 Jan 26.

    PMID: 38280630BACKGROUND
  • Walsh CA, Gorfinkel L, Shmulewitz D, Stohl M, Hasin DS. Use of Lysergic Acid Diethylamide by Major Depression Status. JAMA Psychiatry. 2024 Jan 1;81(1):89-96. doi: 10.1001/jamapsychiatry.2023.3867.

    PMID: 37819655BACKGROUND
  • Ly C, Greb AC, Cameron LP, Wong JM, Barragan EV, Wilson PC, Burbach KF, Soltanzadeh Zarandi S, Sood A, Paddy MR, Duim WC, Dennis MY, McAllister AK, Ori-McKenney KM, Gray JA, Olson DE. Psychedelics Promote Structural and Functional Neural Plasticity. Cell Rep. 2018 Jun 12;23(11):3170-3182. doi: 10.1016/j.celrep.2018.05.022.

    PMID: 29898390BACKGROUND
  • Moliner R, Girych M, Brunello CA, Kovaleva V, Biojone C, Enkavi G, Antenucci L, Kot EF, Goncharuk SA, Kaurinkoski K, Kuutti M, Fred SM, Elsila LV, Sakson S, Cannarozzo C, Diniz CRAF, Seiffert N, Rubiolo A, Haapaniemi H, Meshi E, Nagaeva E, Ohman T, Rog T, Kankuri E, Vilar M, Varjosalo M, Korpi ER, Permi P, Mineev KS, Saarma M, Vattulainen I, Casarotto PC, Castren E. Psychedelics promote plasticity by directly binding to BDNF receptor TrkB. Nat Neurosci. 2023 Jun;26(6):1032-1041. doi: 10.1038/s41593-023-01316-5. Epub 2023 Jun 5.

    PMID: 37280397BACKGROUND
  • Allen N, Jeremiah A, Murphy R, Sumner R, Forsyth A, Hoeh N, Menkes DB, Evans W, Muthukumaraswamy S, Sundram F, Roop P. LSD increases sleep duration the night after microdosing. Transl Psychiatry. 2024 Apr 15;14(1):191. doi: 10.1038/s41398-024-02900-4.

    PMID: 38622150BACKGROUND
  • Hutten NRPW, Quaedflieg CWEM, Mason NL, Theunissen EL, Liechti ME, Duthaler U, Kuypers KPC, Bonnelle V, Feilding A, Ramaekers JG. Inter-individual variability in neural response to low doses of LSD. Transl Psychiatry. 2024 Jul 15;14(1):288. doi: 10.1038/s41398-024-03013-8.

    PMID: 39009578BACKGROUND
  • Bershad AK, Schepers ST, Bremmer MP, Lee R, de Wit H. Acute Subjective and Behavioral Effects of Microdoses of Lysergic Acid Diethylamide in Healthy Human Volunteers. Biol Psychiatry. 2019 Nov 15;86(10):792-800. doi: 10.1016/j.biopsych.2019.05.019. Epub 2019 Jun 3.

    PMID: 31331617BACKGROUND

Related Links

MeSH Terms

Conditions

Depressive Disorder, MajorDepression

Interventions

Lysergic Acid Diethylamide

Condition Hierarchy (Ancestors)

Depressive DisorderMood DisordersMental DisordersBehavioral SymptomsBehavior

Intervention Hierarchy (Ancestors)

Lysergic AcidErgolinesErgot AlkaloidsAlkaloidsHeterocyclic CompoundsHeterocyclic Compounds, 4 or More RingsHeterocyclic Compounds, Fused-Ring

Study Officials

  • Hanna Molla

    University of Chicago

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
interventional
Phase
early phase 1
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
Purpose
OTHER
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

April 22, 2025

First Posted

June 12, 2025

Study Start

June 9, 2025

Primary Completion (Estimated)

June 1, 2026

Study Completion (Estimated)

June 1, 2026

Last Updated

July 3, 2025

Record last verified: 2025-07

Locations