Transcriptional Changes in Isolated Human Adipocytes During Obesity

NCT06907212 | Enrolling By Invitation

• 1 other identifier: S-20200123
• Study Type: observational
• Target: 100
• Locations: 1 country
• Sites: 1

Brief Summary

Adipocytes play an important role in our body through their function as metabolic energy stores in the form of fat deposits, which are stored when metabolic energy is in excess and released when energy levels are low. In severe obesity, adipose tissue grows through the production of more adipocytes from stem cells (hyperplasia) and the increase in the size of existing adipocytes (hypertrophy). This ability of adipocytes, to maintain their functionality during tissue expansion, is critical in determining the development of obesity-related comorbidities. However, not all adipocytes are the same as adipocyte function differs greatly depending on the depot in which they are found. To elucidate the mechanisms underlying adipocyte adaptability, it is necessary to obtain a deep systems biology understanding of how signaling in different types of adipocytes regulates metabolism and function. Currently, this research group is conducting a systems biology analysis of adipocyte plasticity in obesity in mouse models, but adipose tissue biopsies from different depots in humans are lacking and needed to investigate the regulation of adipocyte function and plasticity in humans. Such studies of adipocytes are crucial for the understanding how obesity affects adipose tissue function and leads to comorbidities.

Conditions

Obesity, Abdominal; Adiposity

Keywords

Human primary adipocytes; Obesity; Systems-wide biology; Gene regulatory networks

Outcome Measures

Primary Outcomes (2)

• Adipocytes specific signaling

  RNA sequencing of isolated adipocytes

  June 2024 - Sept 2025

• Gene regulatory networks

  ATAC sequencing of isolated adipocytes to determine the accessible chromatin regions.

  June 2024 - Sept 2025

Secondary Outcomes (3)

• Adipocyte cell size

  Sept 2024 - Dec 2024

• Standard blood tests

  Jan 2025 - Sept 2025

• Adipokine secretion

  Jan 2025 - Sept 2025

Study Arms (1)

Male and female participants with various BMI

Both male and female participants will be included, with the goal of creating a cohort in which all levels of BMI are equally present. Adipocytes of the abdominal subcutaneous and visceral adipose tissue depots will be isolated and sequenced to perform linear regression analysis of transcriptional networks across BMI.

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

Recruitment of subjects will be from patients referred for surgical cholecystectomies, herniotomies or similar operations, at Bispebjerg Hospital.

You may qualify if:

• Age \> 18 years old
• BMI\> 19 kg/m2
• Can receive oral and written information and give appropriate consent

You may not qualify if:

• Active viral hepatitis, HIV positive
• Autoimmune diseases (i.e. lupus)
• Chronic use of corticosteroids (cortisone)
• Unable to give appropriate consent
• Contraindications for biopsy: bleeding tendency.
• Cancer or other comorbidity with an expected survival time of less than 12 months

Sponsors & Collaborators

• University of Southern Denmark: lead
• Bispebjerg Hospital: collaborator

Study Sites (1)

Bispebjerg hospital

Copenhagen, 2400, Denmark

Location

Related Links

• Website of main investigating party

Biospecimen

Retention: SAMPLES WITH DNA

Isolated adipocytes of human adipose tissue

MeSH Terms

Conditions

Obesity, Abdominal; Obesity

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Study Officials

• Susanne Mandrup, PhD

  s.mandrup@bmb.sdu.dk

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: OTHER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: December 16, 2024
• First Posted: April 2, 2025
• Study Start: April 1, 2023
• Primary Completion: June 1, 2025
• Study Completion: September 1, 2025
• Last Updated: April 2, 2025

Record last verified: 2025-03

Data Sharing

• IPD Sharing: Will not share

Locations

DK (1)