Effect of Baseline Cortisol Level on Lipid Peroxidation Products in Young Female Swimmers

NCT06895616 | Completed DMC

• 1 other identifier: SWIMMER- 2024
• Study Type: observational
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

Research group: twenty-four and homogeneous female swimmers, who train swimming intensively and attend the Sports Championship School Complex. In this cohort, baseline serum cortisol levels were considered, and the athletes were divided into two groups: 1) Serum cortisol \<230 (normal): n=14 2) Serum cortisol \>230 ng/ml (high): n=10 In this study, the athletes were subjected to intensive swimming tests in breaststroke crawl (800m + 200m + 50m) with 15 minutes of active rest between test sections in the water. Blood samples took from the cubital vein at three time points: before stress test (pre-exercise), one minute after the end of the test (post-exercise), and after a 3-hour recovery period (3h recovery). Measurements morphology, 4-Hydroxynonenal (4-HNE), 8-isoprostane, cortysol levels were determined using ELISA kits.

Conditions

Stress Oxidative

Outcome Measures

Primary Outcomes (5)

• Changes from baseline in 4-Hydroxynonenal (4-HNE) level.

  Concentration of 4-Hydroxynonenal (4-HNE) \[pg/ml\]. ELISA method by the test manufacturer's instructions

  At rest (before the test), directly after the test, and after a 3-hour recovery period.

• Changes from baseline in 8-isoprostane level.

  Concentration of 8-isoprostane \[ng/mL\]. ELISA method by the test manufacturer's instructions

  At rest (before the test), directly after the test, and after a 3-hour recovery period.

• Changes from baseline in Cortysol level.

  Concentration of cortysol \[ng/mL\]. ELISA method by the test manufacturer's instructions

  At rest (before the test), directly after the test, and after a 3-hour recovery period.

• a manual blood smear

  was made by placing a drop of blood on a slide and then spreading it with a uniform motion. After drying, it was colored by the May Grunwald-Giemsa method according to the procedure. The stained and fixed smear after drying was viewed under a microscope for quantitative and qualitative assessment.

  At rest (before the test), directly after the test, and after a 3-hour recovery period.

• complete blood count

  erythrocytes: RBC \[10\^6/mL\], HGB \[g/dL\], HCT \[%\], MCV \[mm\^3\], MCH \[pg\], MCHC \[g/dL\], RDW \[%\].

  At rest (before the test), directly after the test, and after a 3-hour recovery period.

Secondary Outcomes (7)

• Antropometric characteristic - height

  Day 1 after overall fast

• Antropometric characteristic - weight

  Day 1 after overall fast

• Food record - energy

  Day before the Day 1, in the morning at Day 1

• Food record - protein

  Day before the Day 1, in the morning at Day 1

• Food record - carobhydrates

  Day before the Day 1, in the morning at Day 1

Eligibility Criteria

• Age: 12 Years - 16 Years
• Sex: female
• Healthy Volunteers: No
• Age Groups: Child (0-17)
• Sampling Method: Non-Probability Sample

Study Population

Swimmers attending the Sports Championship School Complex

You may qualify if:

• valid medical examination,
• consent of the legal guardian of the underage athlete to participate in the study,
• Training experience a minimum of three years
• Student of a sports championship school team,
• ompletion of the exercise test in the swimming pool - crawl style intensive test (800 m + 200 m + 50 m) on two test deadline

You may not qualify if:

• antibiotic therapy,
• supplementation, use of any drugs or oral hormonal contraceptives
• health problems within the last months

Sponsors & Collaborators

• Poznan University of Physical Education: lead

Study Sites (1)

Poznań University of Physical Education

Poznan, 61-871, Poland

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Eppendorf tubes with serum.

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: March 19, 2025
• First Posted: March 26, 2025
• Study Start: March 1, 2023
• Primary Completion: December 28, 2023
• Study Completion: December 28, 2023
• Last Updated: March 26, 2025

Record last verified: 2025-03

Data Sharing

• IPD Sharing: Will not share

Locations

PL (1)