NMES Effects on Substrate Metabolism and IGF-1

NCT06850532 | Completed

• 2 other identifiers: MEUFACULTYOFSPORTSCIENCES_2025BAP20172TP32576
• Study Type: interventional
• Target: 24
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of this study is to investigate whether neuromuscular electrostimulation (NMES) training coupled with high-intensity interval training (HIIT) and moderate intensity continuous aerobic training has an effect on the metabolic, cardiovascular and hormonal components compared to respective training protocols without concurrent NMES as a exercise performance enhancement strategy.

Conditions

The Effects of Neuromuscular Electrical Stimulation on Exercise Performance

Outcome Measures

Primary Outcomes (10)

• Body composition via Bioelectrical Impedance Analysis (Tanita 418-MA, Japan)

  Weight (kg) Total body fat (kg) Total muscle mass (kg) The anthropometric parameters were assessed using Bioelectrical impedance analysis (Tanita 418- MA Japan) before all testing sessions.

  From enrollment to the end of treatment at 8 weeks

• Height (cm) via a stadiometer (Holtain Ltd., UK).

  Height was measured through a stadiometer in the standing position (Holtain Ltd., Crymych, UK).

  From enrollment to the end of treatment at 8 weeks

• Heart rate (bpm) via 12-lead ECG

  Heart rate was monitored and recorded throughout baseline and follow-up screenings and all training sessions using 12-lead ECG.

  From enrollment to the end of treatment at 8 weeks

• Blood Lactate Concentration (mmol/L) via Lactate Pro 2 analyzer

  In each GXT testing session (pre-post), blood samples were collected from the earlobe using a Lactate Pro 2 handheld analyzer (LT-1730, Arkray Inc, Kyoto, Japan) to determine blood lactate concentrations testing session (baseline) and at the end of every two minutes interval.

  From enrollment to the end of treatment at 8 weeks

• SERUM INSULIN-LIKE GROWTH FACTOR-1 (IGF-1 ng/mL)

  A 5 mL fasting blood sample was drawn from the brachial vein 30 minutes before baseline and follow-up IVO2max tests to measure serum IGF-1 concentrations. Participants rested for 15 minutes before sample collection. Samples were stored at -80 °C and analyzed post-study. Serum IGF-1 levels were measured using Elabscience ELISA kits (detection range: 1.56-100 ng/mL; sensitivity: 0.94 ng/mL) per the manufacturer's instructions.

  From enrollment to the end of treatment at 8 weeks

• VO2 - Volume of oxygen (ml/kg/min) VCO2 - Volume of carbon dioxide (ml/kg/min)

  During all sessions, VO2 and VCO2 were measured using indirect calorimetry (CareFusion MasterScreen CPX) on an Ergoline Ergoselect 100/200 cycle ergometer. Data was averaged over 15-second intervals during all baseline and follow-up screenings and all training sessions. VO2 and VCO2 were also used to calculate respiratory exchange ratio (RER) during all sessions dividing the VCO2 by VO2.

  From enrollment to the end of treatment at 8 weeks

• Energy expenditure (L/min) via indirect calorimetry (CareFusion MasterScreen CPX).

  During all sessions, energy expenditure was measured using indirect calorimetry (CareFusion MasterScreen CPX). Data was averaged over 15-second intervals, and substrate utilization was calculated using standard formulas. * (GOR)- Oxidation rate of sugar (g/min): 4.585 × VCO2 (L/min) -3.2256 × VO2 (L/min) * (GO)- Oxidation amount of sugar (g): Oxidation rate of sugar × Time (min) * (FOR)- Fat oxidation rate (g/min): 1.695 × VO2 (L/min) -1.701 × VCO2 (L/min) ④ (FO)- Oxidation amount of fat (g): Oxidation rate of fat × Time (min) * (EER)- Energy consumption rate (kcal/min): 3.716 × VO2 (L/min) +1.332 × VCO2 (L/min) ⑥ (EE)- Energy expenditure (kcal): \[3.716 × VO2 (L/min) +1.332 × VCO2 (L/min)\] × Time (min)

  From enrollment to the end of treatment at 8 weeks

• Neuromuscular Electrical Stimulation (NMES) Protocol

  The NMES protocol was administered via a four-channel COMPEX SP4.0 (Medicompex SA, Ecublens, Switzerland) electric muscle stimulator using biphasic symmetric rectangular pulsed currents set at 300 μs. COMPEX self-adhesive electrodes were used during muscle stimulation with the COMPEX device. Positive snap electrodes (5×5 cm) with a membrane depolarization that stimulate a 25 cm2 area of the muscle surface were placed on the proximal insertion of vastus medialis and vastus lateralis. The other negative electrode (10×5 cm), measuring 50 cm2 was placed over the femoral triangle, 1-3 cm below the inguinal ligament.

  From enrollment to the end of treatment at 8 weeks

• Low-frequency Neuromuscular Electrical Stimulation (NMES)

  The low-frequency NMES protocol was administered via a four-channel COMPEX SP4.0 (Medicompex SA, Ecublens, Switzerland) electric muscle stimulator using biphasic symmetric rectangular pulsed currents set at 200 μs. Low-frequency NMES protocol was performed with a duty cycle of 20 seconds on (stimulating) and 20 seconds off (no stimulation) and the pulse width was set at 300 μs (warm-up frequency: 3 Hz, training frequency: 20 Hz, wave: square waveform) to the quadriceps muscle throughout 24 sessions. The duration of training was 30 minutes for sessions 1-6, 36 minutes for sessions 7-12, 42 minutes for sessions 13-18, and 48 minutes for sessions 19-24.

  From enrollment to the end of treatment at 8 weeks

• High-frequency Neuromuscular Electrical Stimulation (NMES)

  High-frequency NMES protocol was administered via a four-channel COMPEX SP4.0 (Medicompex SA, Ecublens, Switzerland) electric muscle stimulator using biphasic symmetric rectangular pulsed currents set at 300 μs. High-freqeuncy NMES protocol consisted of a 5-minute warm-up (65% VO2max) followed by a 1-minute exercise at 120% VO2max and then a 1-minute "loadless" cycling. This interval was repeated 8 times in sessions 1-6 and progressed to 14 repeated intervals by the 24th session. Participants also received an additional NMES treatment with a duty cycle of 12 seconds on (stimulating) and 8 seconds off (no stimulation) and a pulse width of 300 μs (training frequency: 45-60 Hz, wave: square waveform) to the quadriceps muscle throughout 24 sessions.

  From enrollment to the end of treatment at 8 weeks

Study Arms (3)

Assessment of baseline anthropometric, demographic and physiological components

EXPERIMENTAL

Other: IGF-1

Completion of MICT, MICT+NMES, HIIT, and HIIT+NMES protocols

EXPERIMENTAL

Other: IGF-1

Assessment of post-test anthropometric, demographic and physiological components

EXPERIMENTAL

Other: IGF-1

Interventions

IGF-1 OTHER

The effects of NMES coupled with HIIT and MICT on IGF-1 and substrate metabolism has not been studied earlier.

Assessment of baseline anthropometric, demographic and physiological components; Assessment of post-test anthropometric, demographic and physiological components; Completion of MICT, MICT+NMES, HIIT, and HIIT+NMES protocols

Eligibility Criteria

• Age: 18 Years - 30 Years
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• \- All participants were healthy with no musculoskeletal injuries and had not participated in lower-body resistance training for six months prior to the study.

You may not qualify if:

• Individuals with musculoskeletal injuries and had participated in lower-body resistance training for six months prior to the study has been excluded from the enrollement for the current study.

Sponsors & Collaborators

• Mersin University: lead

Study Sites (1)

Mersin University

Yenişehir, Mersin, 33343, Turkey (Türkiye)

Location

MeSH Terms

Interventions

Insulin-Like Growth Factor I

Intervention Hierarchy (Ancestors)

Somatomedins; Insulin-Like Peptides; Intercellular Signaling Peptides and Proteins; Peptides; Amino Acids, Peptides, and Proteins; Blood Proteins; Proteins; Biological Factors

Study Officials

• Nevzat Demirci, PhD

  Mersin University

  STUDY DIRECTOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: SCREENING
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: ASSISTANT PROFESSOR

Study Record Dates

• First Submitted: February 20, 2025
• First Posted: February 27, 2025
• Study Start: March 17, 2017
• Primary Completion: February 13, 2019
• Study Completion: February 13, 2019
• Last Updated: February 27, 2025

Record last verified: 2025-02

Data Sharing

• IPD Sharing: Will share
• Shared Documents: STUDY PROTOCOL, SAP, ICF

Locations

TU (1)