Molecules Associated with Pediatric Obesity

NCT06775990 | Completed DMC

• 1 other identifier: 34574
• Study Type: observational
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

Childhood obesity is linked to obesity in adulthood and causes severe problems, particularly due to its relationship with metabolic syndrome, diabetes, cancer, and cardiovascular diseases. The determination of markers and receptor profiles related to parameters contributing to energy homeostasis in healthy children with a normal body mass index and children with obesity will guide the diagnosis and treatment of pediatric obesity. The aim was to determine possible relationships by evaluating markers contributing to energy homeostasis in healthy individuals and children with obesity. Anthropometric measurements of the children were obtained. The serum leptin, leptin receptor (LEPR), ghrelin, acylated ghrelin, deacylated ghrelin, ghrelin receptor (GHSR), kisspeptin (KISS1), kisspeptin receptor (KISS1R), preptin, peroxisome proliferator activated receptor gamma (PPARγ), nod-like receptor pyrin domain-containing 3 (NLRP3), and interleukin-18 (IL-18) levels of thirty children with obesity and thirty healthy children were determined using ELISA.

Conditions

OBESITY

Keywords

Child; Obesity; Obesity parameters and receptors

Outcome Measures

Primary Outcomes (1)

• Obesity BMI values according to WHO criteria accepted by the Ministry of Health

  Two groups were formed from 30 children with obesity BMI values above the 95th percentile according to the WHO criteria accepted by the Ministry of Health and 30 children with healthy BMI values between the 85th and 15th percentiles who constituted the control group. Weight in kilograms Height in meters weight and height will be combined to report BMI in kg/m\^2

  6 months

Study Arms (2)

Healthy control

Children between the ages of 06-18 years without acute or chronic diseases and without major congenital anomalies were included in the study. Two groups were formed from 30 obese children with BMI values above the 95th percentile according to the WHO criteria accepted by the Ministry of Health and 60 healthy children with BMI values between the 85th and 15th percentiles who constituted the control group. Of the 60 children, 35 were girls and 25 were boys. Serum leptin, leptin receptor (LEPR), ghrelin, acylated ghrelin, deacylated ghrelin, ghrelin receptor (GHSR), kisspeptin (KISS1), kisspeptin receptor (KISS1R), preptin, Peroxisome proliferator-activated receptor gamma (PPAR γ), nod-like receptor pyrin domain-containing 3 (NLRP3), interleukin-18 (IL-18) levels were determined by ELISA in thirty obese and thirty healthy children.

Other: Blood sample

Obese children.

Children between the ages of 06-18 years without acute or chronic diseases and without major congenital anomalies were included in the study. Two groups were formed from 30 obese children with BMI values above the 95th percentile according to the WHO criteria accepted by the Ministry of Health and 60 healthy children with BMI values between the 85th and 15th percentiles who constituted the control group. Of the 60 children, 35 were girls and 25 were boys. Serum leptin, leptin receptor (LEPR), ghrelin, acylated ghrelin, deacylated ghrelin, ghrelin receptor (GHSR), kisspeptin (KISS1), kisspeptin receptor (KISS1R), preptin, Peroxisome proliferator-activated receptor gamma (PPAR γ), nod-like receptor pyrin domain-containing 3 (NLRP3), interleukin-18 (IL-18) levels were determined by ELISA in thirty obese and thirty healthy children.

Other: Blood sample

Interventions

Blood sample OTHER

Children were not given any medication in the study. Peripheral blood samples from the children were collected, centrifuged and stored in the biochemistry laboratory in deep freezers at -80 degree celcius. From stored serum samples leptin, leptin receptor (LEPR), ghrelin, acylated ghrelin, deacylated ghrelin, ghrelin receptor (GHSR), kisspeptin (KISS1), kisspeptin receptor (KISS1R), preptin, Peroxisome proliferator-activated receptor gamma (PPAR γ), nod-like receptor pyrin domain-containing 3 (NLRP3), interleukin-18 (IL-18) levels were determined by ELISA in thirty obese and thirty healthy children.

Healthy control; Obese children.

Eligibility Criteria

• Age: 6 Years - 18 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64)
• Sampling Method: Probability Sample

Study Population

Children between the ages of 6 and 18 years without acute or chronic diseases and without major congenital anomalies were included in the study. Peripheral blood samples were collected from children admitted to Tekirdağ Namık Kemal University Faculty of Medicine, Department of Pediatrics after 8-12 hours of fasting. Two groups were formed from 30 children with obesity BMI values above the 95th percentile according to the WHO criteria accepted by the Ministry of Health and 30 children with healthy BMI values between the 85th and 15th percentiles who constituted the control group. Of the 60 children, 35 were girls and 25 were boys.

You may qualify if:

• Children aged 6-18 years were included in the study
• obese children with BMI values above the 95th percentile according to WHO criteria and 30 healthy children with BMI values between the 85th and 15th percentiles and 60 healthy children with anorexia were included in the study
• Children without acute or chronic diseases and without major congenital anomalies were included in the study.

You may not qualify if:

• Be over 18 years of age
• Presence of conditions that may cause secondary obesity, such as hypothyroidism or polycystic ovary syndrome
• Children with previous bariatric surgery or repeated diet attempts;
• Children with a serious acute or chronic illness (active infection, heart failure, cardiovascular disease, cerebrovascular disease, acute renal failure, chronic renal failure, chronic lung disease, malignancy) were excluded.

Sponsors & Collaborators

• Istanbul University - Cerrahpasa: lead
• Scientific Research Project Coordination Unit of Istanbul University-Cerrahpasa: collaborator

Study Sites (1)

Namik Kemal University

Tekirdağ, Tekirdağ, 59010, Turkey (Türkiye)

Location

Related Links

• NIH Case Reports Metabolism . 2021 Mar:116:154438. doi: 10.1016/j.metabol.2020.154438. Epub 2020 Nov 19.

Biospecimen

Retention: SAMPLES WITH DNA

Serum samples were stored at -80 degrees Celsius.

MeSH Terms

Conditions

Obesity

Interventions

Blood Specimen Collection

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms

Intervention Hierarchy (Ancestors)

Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Surgical Procedures, Operative; Investigative Techniques

Study Officials

• Ezgi Kürkçü Kahraman, Assist Prof.

  Istanbul Beykent University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: PROSPECTIVE
• Target Duration: 2 Days
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Prof.

Study Record Dates

• First Submitted: January 4, 2025
• First Posted: January 15, 2025
• Study Start: June 4, 2020
• Primary Completion: September 6, 2021
• Study Completion: June 8, 2022
• Last Updated: January 17, 2025

Record last verified: 2025-01

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)