Effect of Exercise on Irisin Levels of Hypothyroid Rats

NCT06699069 | Completed DMC

• 1 other identifier: IRC/20/232 Fatima Iqbal
• Study Type: interventional
• Target: 30
• Locations: 1 country
• Sites: 1

Brief Summary

The aim of study was to evaluate the role of exercise on serum irisin levels in hypothyroid rats.

Conditions

Hypothyroidism

Keywords

Exercise; Irisin; Hypothyroidism

Outcome Measures

Primary Outcomes (7)

• Serum Triiodothyronine T3

  Estimation of serum free triiodothyronine (FT3) levels (ng/ml) by radioimmunoassay

  11th week

• Serum Thyroxine

  Estimation of serum free thyroxin (FT4) levels (μg/dl) by radioimmunoassay

  11th week

• Serum Irisin

  Estimation of serum irisin levels (μg/ml) by using enzyme - linked immunosorbent assay (ELISA) using irisin competitive ELISA Kit

  11th week

• Serum Thyroid Stimulating Hormone

  By radioimmunoassay

  11th week

• Serum Triglycerides (TG)

  By enzymatic method using micro lab analyzer

  11th week

• Serum High Density Lipoproteins (HDL)

  Determined by precipitating method using micro lab analyzer

  11th week

• Serum Low Density Lipoproteins (LDL)

  LDL= Total cholesterol-HDL-TG/5

  11th week

Study Arms (3)

Group I (Control or Euthyroid) total number of rats=20

EXPERIMENTAL

Fed on normal standard diet

Other: Control or Euthyroid group

Group II (Hypothyroid) total number of rats=20

EXPERIMENTAL

Experimental hypothyroidism will be induced by administration of propylthiouracil for three weeks in the form of tablets diluted in water

Other: Hypothyroid group

Group III (Hypothyroid received exercise) total number of rats=20

EXPERIMENTAL

Rats will be subjected to chronic swimming. The swimming protocol will consist of 8 weeks of low-intensity swimming exercise.

Other: Hypothyroid group received exercise

Interventions

Control or Euthyroid group OTHER

Fed on normal standard diet

Group I (Control or Euthyroid) total number of rats=20

Hypothyroid group OTHER

Experimental hypothyroidism will be induced by administration of propylthiouracil for three weeks in the form of tablets diluted in water

Group II (Hypothyroid) total number of rats=20

Hypothyroid group received exercise OTHER

Rats will be subjected to chronic swimming. The swimming protocol will consist of 8 weeks of low-intensity swimming exercise. The exercise groups will be adapted to a round water tank (70 cm × 70 cm) containing water at 28 ± 2°C for 3 days (10-45 min/day). The rats will then begin swimming, 45 min/day for the first two weeks at 10am and 60 min/day for the last six weeks at 10am

Group III (Hypothyroid received exercise) total number of rats=20

Eligibility Criteria

• Age: 6 Weeks - 8 Weeks
• Gender Eligibility Details: Male Sprague Dawley Rats
• Healthy Volunteers: No
• Age Groups: Child (0-17)

You may qualify if:

• Male adult rats weighing approx. 200-250gms
• to 8 week old rats

You may not qualify if:

• Female rats will be excluded.
• Rats with any obvious pathology
• The rats weighing \>200gm

Sponsors & Collaborators

• Riphah International University: lead

Study Sites (1)

National Institute of Health

Islamabad, Federal, 44000, Pakistan

Location

Related Publications (12)

• Martinez Munoz IY, Camarillo Romero EDS, Garduno Garcia JJ. Irisin a Novel Metabolic Biomarker: Present Knowledge and Future Directions. Int J Endocrinol. 2018 Oct 9;2018:7816806. doi: 10.1155/2018/7816806. eCollection 2018.

  PMID: 30402097 BACKGROUND

• Korta P, Pochec E, Mazur-Bialy A. Irisin as a Multifunctional Protein: Implications for Health and Certain Diseases. Medicina (Kaunas). 2019 Aug 15;55(8):485. doi: 10.3390/medicina55080485.

  PMID: 31443222 BACKGROUND

• Gizaw M, Anandakumar P, Debela T. A Review on the Role of Irisin in Insulin Resistance and Type 2 Diabetes Mellitus. J Pharmacopuncture. 2017 Dec;20(4):235-242. doi: 10.3831/KPI.2017.20.029. Epub 2017 Oct 10.

  PMID: 30151293 BACKGROUND

• Uc ZA, Gorar S, Mizrak S, Gullu S. Irisin levels increase after treatment in patients with newly diagnosed Hashimoto thyroiditis. J Endocrinol Invest. 2019 Feb;42(2):175-181. doi: 10.1007/s40618-018-0899-8. Epub 2018 May 18.

  PMID: 29777516 BACKGROUND

• Maalouf GE, El Khoury D. Exercise-Induced Irisin, the Fat Browning Myokine, as a Potential Anticancer Agent. J Obes. 2019 Apr 1;2019:6561726. doi: 10.1155/2019/6561726. eCollection 2019.

  PMID: 31065382 BACKGROUND

• Deshmukh V, Farishta F, Bhole M. Thyroid Dysfunction in Patients with Metabolic Syndrome: A Cross-Sectional, Epidemiological, Pan-India Study. Int J Endocrinol. 2018 Dec 25;2018:2930251. doi: 10.1155/2018/2930251. eCollection 2018.

  PMID: 30675157 BACKGROUND

• Abdel Moety DA. Evaluation of Serum Irisin and Creatine Kinase Levels in Hypo- and Hyperthyroid Rats. Al-Azhar Med J. 2017;46(1):163-74.

  BACKGROUND

• Ates I, Altay M, Topcuoglu C, Yilmaz FM. Circulating levels of irisin is elevated in hypothyroidism, a case-control study. Arch Endocrinol Metab. 2016 Apr;60(2):95-100. doi: 10.1590/2359-3997000000077. Epub 2015 Jul 21.

  PMID: 26201007 BACKGROUND

• Yang N, Zhang H, Gao X, Miao L, Yao Z, Xu Y, Wang G. Role of irisin in Chinese patients with hypothyroidism: an interventional study. J Int Med Res. 2019 Apr;47(4):1592-1601. doi: 10.1177/0300060518824445. Epub 2019 Feb 6.

  PMID: 30722716 BACKGROUND

• Rochlani Y, Pothineni NV, Kovelamudi S, Mehta JL. Metabolic syndrome: pathophysiology, management, and modulation by natural compounds. Ther Adv Cardiovasc Dis. 2017 Aug;11(8):215-225. doi: 10.1177/1753944717711379. Epub 2017 Jun 22.

  PMID: 28639538 BACKGROUND

• Teixeira PFDS, Dos Santos PB, Pazos-Moura CC. The role of thyroid hormone in metabolism and metabolic syndrome. Ther Adv Endocrinol Metab. 2020 May 13;11:2042018820917869. doi: 10.1177/2042018820917869. eCollection 2020.

  PMID: 32489580 BACKGROUND

• Kurdiova T, Balaz M, Vician M, Maderova D, Vlcek M, Valkovic L, Srbecky M, Imrich R, Kyselovicova O, Belan V, Jelok I, Wolfrum C, Klimes I, Krssak M, Zemkova E, Gasperikova D, Ukropec J, Ukropcova B. Effects of obesity, diabetes and exercise on Fndc5 gene expression and irisin release in human skeletal muscle and adipose tissue: in vivo and in vitro studies. J Physiol. 2014 Mar 1;592(5):1091-107. doi: 10.1113/jphysiol.2013.264655. Epub 2013 Dec 2.

  PMID: 24297848 BACKGROUND

MeSH Terms

Conditions

Hypothyroidism; Motor Activity

Condition Hierarchy (Ancestors)

Thyroid Diseases; Endocrine System Diseases; Behavior

Study Officials

• Shazia Ali, PhD

  Riphah International University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: February 2, 2021
• First Posted: November 21, 2024
• Study Start: February 20, 2021
• Primary Completion: January 30, 2022
• Study Completion: January 30, 2022
• Last Updated: November 21, 2024

Record last verified: 2024-11

Data Sharing

• IPD Sharing: Will not share

Locations

PA (1)