The Effect of Aerobic and Anaerobic Exercise on Oxidative Stress and Cellular Fitness in Healthy Trained Young Men

NCT06582862 | Enrolling By Invitation

• 1 other identifier: 24-05-0791
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

This study aims to analyze the effects of aerobic and anaerobic exercise on health at the molecular level. By examining these effects, the study seeks to provide the public with insights into which types of exercise offer the most significant health benefits. Participants will engage in aerobic and anaerobic exercises for one month, with 5 mL of venous blood collected by experienced phlebotomists both before and after the exercise period. While blood collection may cause mild discomfort and temporary bruising, these symptoms should subside within a few days. The collected blood will be processed to separate plasma and leukocytes for the assessment of oxidative damage, lipid profile and cellular fitness parameters. The oxidative damage markers to be measured include malondialdehyde (MDA) levels, H2O2, and antioxidants such as total antioxidant capacity, superoxide dismutase, and glutathione peroxidase. Cellular fitness will be evaluated by measuring mitochondrial biogenesis markers (succinate dehydrogenase and PGC-1A), ATP levels, and ATPase inhibitor levels. The benefits for the participants, they will receive include a laboratory assessment to evaluate cell damage, lipid profiles, and mitochondrial function. Additionally, the study results will help identify the most beneficial type of physical exercise for optimal health.

Conditions

Health, Subjective

Outcome Measures

Primary Outcomes (5)

• Concentration of Malondialdehyde (MDA)

  MDA in nmoL/mL is measured using spectrophotometer from the subject's plasma

  1 month

• Concentration of Hydrogen Peroxide

  Hydrogen peroxide in mmol/L is measured using spectrophotometer from the subject's plasma

  1 month

• Concentration of Superoxide dismutase (SOD)

  SOD in U/mL is measured using spectrophotometer from the subject's plasma

  1 month

• Concentration of Total Antioxidant Capacity

  Total Antioxidant Capacity in U/mL is measured using spectrophotometer from the subject's plasma

  1 month

• Concentration of Glutathione Peroxidase (GPx)

  GPx in in U/mL is measured using spectrophotometer from the subject's plasma

  1 month

Secondary Outcomes (4)

• Concentration of Succinate Dehydrogenase (SDH)

  1 month

• Concentration of Peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha

  1 month

• Concentration of Adenosine triphosphate (ATP)

  1 month

• Concentration of ATPase inhibitor

  1 month

Study Arms (2)

Pre aerobic exercise treatment

EXPERIMENTAL

Subject will do aerobic exercise 3 times a week for 1 month. The training program includes the following: Week 1 consists of interval running (2 sets of 10 repetitions of 400 meters each) with 3 minutes of rest between repetitions and 10 minutes of rest between sets. Week 2 transitions to continuous running for 15-20 minutes. Week 3 involves 30 minutes of cross-country running. In Week 4, the program returns to interval running as in Week 1.

Other: Exercise

Pre anaerobic exercise treatment

EXPERIMENTAL

Subject will do anerobic exercise 3 times a week for 1 month. The sprint training program includes the following: In Weeks 1 and 3, perform sprints of 20 m, 40 m, 60 m, and 80 m, with 2 sets of 5 repetitions each, allowing 5 minutes of rest between repetitions and 10 minutes of rest between sets. In Weeks 2 and 4, run 2 sets of 5 repetitions of 50 m sprints, with 5 minutes of rest between repetitions and 10 minutes between sets.

Other: Exercise

Interventions

Exercise OTHER

Subjects will do aerobic and anaerobic exercise

Pre aerobic exercise treatment; Pre anaerobic exercise treatment

Eligibility Criteria

• Age: 18 Years - 23 Years
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Male, aged 18-23.
• body weight of 56-70 kg and a height of 158-175 cm.
• Systolic blood pressure values below 130 mmHg, and diastolic blood pressure below 90 mmHg.
• maximum oxygen consumption (VO2max) is calculated to be greater than 40 mL/(kg-minute).

You may not qualify if:

• fever
• having chronic diseases (heart disease, lung disease)
• smoking

Sponsors & Collaborators

• Indonesia University: lead

Study Sites (1)

Faculty of Medicine Universitas Indonesia

Jakarta Pusat, Jakarta Special Capital Region, 10430, Indonesia

Location

MeSH Terms

Interventions

Exercise

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Novi Hardiany

  Fakultas Kedokteran Universitas Indonesia

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Research Coordinator

Model Details: Participants will be divided into 2 groups. One group will do aerobic exercise and other group will do anaerobic exercise

Study Record Dates

• First Submitted: August 27, 2024
• First Posted: September 3, 2024
• Study Start: June 1, 2024
• Primary Completion: September 30, 2024
• Study Completion: November 30, 2024
• Last Updated: September 3, 2024

Record last verified: 2024-08

Data Sharing

• IPD Sharing: Will not share

Locations

ID (1)