NCT06576804

Brief Summary

Eccentric cycling exercise (ECE) features lower metabolic demand and higher improvement of muscle strength compared to traditional concentric cycling exercise (CCE). Mitochondria can regulate energy metabolism and adaptive immune quality in T lymphocytes. However, the effects of ECE on mitochondrial functions in T-lymphocytes have not yet been established. Method: A total of 33 healthy sedentary males were randomized and divided into ECE (n=11), CCE (n=11), and control groups (n=11). These subjects progressively performed CCE or ECE from 60% to 80% maximal workload on a bicycle ergometer for 40 min/day, 5 days/week for 6 weeks. A graded exercise testing and an isokinetic strength test were conducted to evaluate cardiopulmonary fitness and muscle strength, respectively. Phenotypes and mitochondrial respiratory capacity in T lymphocyte were analyzed using flow cytometry and high-resolution respirometer, respectively.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
33

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Jan 2021

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

January 1, 2021

Completed
1 year until next milestone

Primary Completion

Last participant's last visit for primary outcome

January 1, 2022

Completed
7 months until next milestone

Study Completion

Last participant's last visit for all outcomes

August 1, 2022

Completed
2.1 years until next milestone

First Submitted

Initial submission to the registry

August 20, 2024

Completed
9 days until next milestone

First Posted

Study publicly available on registry

August 29, 2024

Completed
Last Updated

August 29, 2024

Status Verified

August 1, 2024

Enrollment Period

1 year

First QC Date

August 20, 2024

Last Update Submit

August 27, 2024

Conditions

Eccentric Exercise Training

Keywords

Eccentric cyclingMitochondriaLymphocyte

Outcome Measures

Primary Outcomes (1)

  • Mitochondrial function of lymphocyte

    Evaluate bioenergetic health index (BHI) of mitochondria in lymphocyte by using a high resolution respirometer.

    12 weeks

Secondary Outcomes (1)

  • Cardiopulmonary fitness

    12 weeks

Study Arms (3)

Eccentric cycling training

EXPERIMENTAL

Moderate to high intensity cycling training in eccentric type

Behavioral: Eccentric cycling training

Concentric cycling training

EXPERIMENTAL

Moderate to high intensity cycling training in concentric type (intensity matched the eccentric training)

Behavioral: Concentric cycling training

Control group

NO INTERVENTION

without receiving any exercise training

Interventions

Eccentric cycling trainingBEHAVIORAL

Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer. Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase. Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week

Eccentric cycling training
Concentric cycling trainingBEHAVIORAL

Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer. Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase. Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week

Concentric cycling training

Eligibility Criteria

Age20 Years - 30 Years
Sexmale
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Sedentary lifestyle.
  • Nonsmokers, nonusers of medications/vitamins.
  • No any cardiopulmonary/hematological risks.

You may not qualify if:

  • Have regular exercise habits (i.e., exercise frequency once per week, duration \>20 min).
  • Have retabolic or musculoskeletal disease.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Chang Gung University

Taoyuan District, 333, Taiwan

Location

Related Publications (4)

  • LaStayo PC, Ewy GA, Pierotti DD, Johns RK, Lindstedt S. The positive effects of negative work: increased muscle strength and decreased fall risk in a frail elderly population. J Gerontol A Biol Sci Med Sci. 2003 May;58(5):M419-24. doi: 10.1093/gerona/58.5.m419.

    PMID: 12730250RESULT

  • Hody S, Croisier JL, Bury T, Rogister B, Leprince P. Eccentric Muscle Contractions: Risks and Benefits. Front Physiol. 2019 May 3;10:536. doi: 10.3389/fphys.2019.00536. eCollection 2019.

    PMID: 31130877RESULT

  • Touron J, Costes F, Coudeyre E, Perrault H, Richard R. Aerobic Metabolic Adaptations in Endurance Eccentric Exercise and Training: From Whole Body to Mitochondria. Front Physiol. 2021 Jan 27;11:596351. doi: 10.3389/fphys.2020.596351. eCollection 2020.

    PMID: 33584331RESULT

  • Tsai HH, Chang SC, Chou CH, Weng TP, Hsu CC, Wang JS. Exercise Training Alleviates Hypoxia-induced Mitochondrial Dysfunction in the Lymphocytes of Sedentary Males. Sci Rep. 2016 Oct 12;6:35170. doi: 10.1038/srep35170.

    PMID: 27731374RESULT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
OUTCOMES ASSESSOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

August 20, 2024

First Posted

August 29, 2024

Study Start

January 1, 2021

Primary Completion

January 1, 2022

Study Completion

August 1, 2022

Last Updated

August 29, 2024

Record last verified: 2024-08

Data Sharing

IPD Sharing
Will not share

Locations

TW(1)