NCT06472765

Brief Summary

The central premise of this study is that the intricate balance and diversity of the vaginal microbiome plays a pivotal role in the onset, progression, and severity of various gynecological conditions. Specifically, the research aims to investigate how imbalances in microbial communities, such as the overgrowth of pathogenic bacteria or the depletion of beneficial ones, are linked to conditions like Bacterial Vaginosis, Candidiasis, Urinary Tract Infections, Vaginal Atrophy, and others. By employing PCR testing and the outcomes of next-generation sequencing (NGS) of the microbiome, the study seeks to identify distinct microbial profiles and patterns that are characteristic of each condition. This nuanced understanding is expected to lead to more accurate and early diagnosis, facilitating personalized and effective treatment strategies that go beyond the conventional, often indiscriminate use of antibiotics.

Trial Health

78
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
3,250

participants targeted

Target at P75+ for all trials

Timeline
25mo left

Started May 2024

Longer than P75 for all trials

Geographic Reach
2 countries

21 active sites

Status
enrolling by invitation

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress49%
May 2024May 2028

Study Start

First participant enrolled

May 16, 2024

Completed
1 month until next milestone

First Submitted

Initial submission to the registry

June 18, 2024

Completed
7 days until next milestone

First Posted

Study publicly available on registry

June 25, 2024

Completed
2.9 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 15, 2027

Expected
1 year until next milestone

Study Completion

Last participant's last visit for all outcomes

May 15, 2028

Last Updated

July 1, 2025

Status Verified

June 1, 2025

Enrollment Period

3 years

First QC Date

June 18, 2024

Last Update Submit

June 29, 2025

Conditions

Outcome Measures

Primary Outcomes (1)

  • Microbiome Analysis

    To analyze the vaginal microbiome in patients with diverse vaginal health conditions using next-generation sequencing (NGS) and a 56-panel PCR test to understand microbial diversity and implications for disease management.

    Day 1

Secondary Outcomes (1)

  • Registry

    Day 1

Study Arms (13)

Premenopausal Healthy

Premenopausal Healthy

Diagnostic Test: Vaginal Swab Collection

Perimenopausal Healthy

Perimenopausal Healthy

Diagnostic Test: Vaginal Swab Collection

Postmenopausal Healthy

Postmenopausal Healthy

Diagnostic Test: Vaginal Swab Collection

Bacterial Vaginosis

Bacterial Vaginosis

Diagnostic Test: Vaginal Swab Collection

Candidiasis

Yeast Infections

Diagnostic Test: Vaginal Swab Collection

Urinary Tract Infection

Premenopausal and Postmenopausal Women

Diagnostic Test: Vaginal Swab Collection

Genitourinary Syndrome of Menopause

Vaginal Atrophy

Diagnostic Test: Vaginal Swab Collection

Lichen Sclerosus/Planus

Lichen Sclerosus/Planus

Diagnostic Test: Vaginal Swab Collection

Vulvodynia

Vulvodynia

Diagnostic Test: Vaginal Swab Collection

Endometriosis

Endometriosis

Diagnostic Test: Vaginal Swab Collection

Ureaplasma

Ureaplasma

Diagnostic Test: Vaginal Swab Collection

Desquamative Inflammatory Vaginitis (DIV)

Desquamative Inflammatory Vaginitis (DIV)

Diagnostic Test: Vaginal Swab Collection

Cancer

Group 1: Breast cancer patients diagnosed at stages 1-3, post-menopausal (due to surgical, chemical reasons, or age-related natural processes), and currently receiving anti-estrogen treatment for a minimum of 90 days. Group 2: Breast cancer patients diagnosed at stages 1-3, pre-menopausal, and undergoing anti-estrogen endocrine therapy for at least 90 days. Group 3: Participants diagnosed with any type of cancer at stages 1-3, not receiving anti-estrogen therapy, but have been using vaginal estrogen, prasterone, or DHEA for 90 days or more. Should not be on systemic hormone replacement therapy (estrogen with or without progesterone). Group 4: Diagnosed with any type of cancer at stages 1-3, and should not be receiving any anti-estrogen therapy or using vaginal estrogen, prasterone, or DHEA. These individuals must not be on systemic hormone replacement therapy. Group 5: Participants diagnosed at stage 4

Diagnostic Test: Vaginal Swab Collection

Interventions

Vaginal Swab Collection

Bacterial VaginosisCancerCandidiasisDesquamative Inflammatory Vaginitis (DIV)EndometriosisGenitourinary Syndrome of MenopauseLichen Sclerosus/PlanusPerimenopausal HealthyPostmenopausal HealthyPremenopausal HealthyUreaplasmaUrinary Tract InfectionVulvodynia

Eligibility Criteria

Age18 Years - 89 Years
Sexfemale(Gender-based eligibility)
Gender Eligibility DetailsCis-gender women
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

This study's substantial sample size of 3,250 participants is statistically justified to ensure a diverse and representative sample of the population, providing increased statistical power for detecting variations in the vaginal microbiome. This approach not only allows for a comprehensive understanding of microbiome variability but also ensures the ethical utility of the research, making the findings significantly beneficial for a broad demographic. The following are the subgroups of conditions/individuals that will be sampled, each 250 swabs: 1. Premenopausal Healthy 2. Perimenopausal Healthy 3. Postmenopausal Healthy 4. Bacterial Vaginosis 5. Candidiasis (Yeast Infections) 6. Urinary Tract Infection (premenopausal and postmenopausal) 7. Genitourinary Syndrome of Menopause 8. Lichen Sclerosus/Planus 9. Vulvodynia 10. Endometriosis 11. Ureaplasma 12. Desquamative Inflammatory Vaginitis (DIV) 13. Cancer (various types)

You may qualify if:

  • Healthy cis-gender women between ages 18 and 89

You may not qualify if:

  • Pregnant or trying to conceive (i.e. IVF, IUI)

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (21)

Women's Pelvic Health Center

Fullerton, California, 92835, United States

Location

Claris Health

Los Angeles, California, 90064, United States

Location

University of California Los Angeles Department of Radiation Oncology

Los Angeles, California, 90095, United States

Location

Pelvic Pain Rehab

Pasadena, California, 91106, United States

Location

Healthy Mothers, Healthy Babies Coalition of Hawaii

Honolulu, Hawaii, 96817, United States

Location

Rachel Rubin MD PLLC

Bethesda, Maryland, 20814, United States

Location

Dr. Christina Enzmann at Easton Advanced Aesthetics and Regenerative Medicine

Easton, Maryland, 21601, United States

Location

Empowered Midwifery and Wellness LLC

Alexandria, Minnesota, 56308, United States

Location

Xena Health

Henderson, Nevada, 89074, United States

Location

Empire OBGYN

Kenmore, New York, 14217, United States

Location

Gary H Goldman, MD

New York, New York, 10128, United States

Location

Camel City Women's Wellness

Winston-Salem, North Carolina, 27101, United States

Location

UH Hospitals

Mayfield Heights, Ohio, 44124, United States

Location

Northwest Obstetrics and Gynecology

Oklahoma City, Oklahoma, 73112, United States

Location

Haven Center for Sexual Medicine & Vulvovaginal Disorders

Tulsa, Oklahoma, 74137, United States

Location

Center of the Health

Corvallis, Oregon, 97330, United States

Location

Amber Wellness Group

Portland, Oregon, 97213, United States

Location

Center for Pelvic Medicine

Bryn Mawr, Pennsylvania, 19010, United States

Location

North Houston Gynecologic Oncology Surgeons, PLLC

Shenandoah, Texas, 77384, United States

Location

Hello Climax

Virginia Beach, Virginia, 23455, United States

Location

Integrative Naturopathic Medical Centre

Vancouver, British Columbia, V6H 3X8, Canada

Location

Biospecimen

Retention: SAMPLES WITH DNA

Vaginal swab for Next Generation Sequencing and Polymerase Chain Reaction tests.

MeSH Terms

Conditions

Vaginosis, BacterialCandidiasisUrinary Tract InfectionsLichen Sclerosus et AtrophicusLichen PlanusVulvodyniaEndometriosisUreaplasma InfectionsNeoplasms

Condition Hierarchy (Ancestors)

Bacterial InfectionsBacterial Infections and MycosesInfectionsVaginitisVaginal DiseasesGenital Diseases, FemaleFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesGenital DiseasesMycosesUrologic DiseasesMale Urogenital DiseasesLichenoid EruptionsSkin Diseases, PapulosquamousSkin DiseasesSkin and Connective Tissue DiseasesVulvar DiseasesMycoplasmatales InfectionsGram-Negative Bacterial Infections

Study Officials

  • Kimberly Capone, PhD

    Vaginal Biome Science

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
CROSS SECTIONAL
Target Duration
1 Day
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 18, 2024

First Posted

June 25, 2024

Study Start

May 16, 2024

Primary Completion (Estimated)

May 15, 2027

Study Completion (Estimated)

May 15, 2028

Last Updated

July 1, 2025

Record last verified: 2025-06

Locations