Interleukin- 17 (IL-17), Bcl-3, and NF-kappa-B Inhibitor Zeta (IκBζ) Expression Levels in the Psoriasis Patients

NCT06408454 | Completed

• 1 other identifier: KSU-ONERTALMAC-01
• Study Type: observational
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

This study aimed to compare the levels of IL-17 (interleukin- 17), Bcl-3 (B-cell lymphoma 3-encoded protein), and IκBζ (NF-kappa-B inhibitor zeta) in the gingival crevicular fluid of psoriatic and healthy individuals and the clinical parameters such as periodontal health, gingival index, plaque index and mobility (using periotest device)in the patient and control groups.

Conditions

Psoriasis; Periodontal Diseases

Keywords

psoriasis; gene expression; periodontal health

Outcome Measures

Primary Outcomes (1)

• Level of Gene expression

  In the real-time polymerase chain reaction (PCR) results of the β-actin gene, which was used as a housekeeping gene in the study, the automatic threshold value was determined by the device as 0.02. It was observed that all samples were above the threshold value and gave a ct value. According to the housekeeping gene, IL-17, IκBζ, and Bcl-3gene expression values were determined.

  Sample collection during examination, approximately 2 months

Secondary Outcomes (4)

• Plaque index

  Baseline, week 2

• Gingival index

  Baseline, week 2

• Clinical attachment level

  Baseline, week 2

• Tooth Mobility

  Baseline, week 2

Study Arms (2)

Psoriasis patients

IL-17, Bcl-3, IκBζ gene expression levels were examined

Diagnostic Test: Gingival crevicular fluid collection; Diagnostic Test: periodontal test

Control group

IL-17, Bcl-3, IκBζ gene expression levels were examined

Diagnostic Test: Gingival crevicular fluid collection; Diagnostic Test: periodontal test

Interventions

Gingival crevicular fluid collection DIAGNOSTIC_TEST

Samples were taken from two mandibular molar teeth. Before sampling, the sites were isolated with cotton rolls and then carefully dried with an air syringe to avoid saliva contamination. GCF was collected using sterile periodontal strips by gently inserting them into the gingival sulcus or pocket until slight resistance was noticed. After 30 s, the strips were collected into sterile tubes and immediately transported to the laboratory for storage (-40 °C) and posterior analysis.

Control group; Psoriasis patients

periodontal test DIAGNOSTIC_TEST

A periodontist conducted the periodontal evaluation, which included a comprehensive assessment of clinical periodontal status, a full mouth manual periodontal charting, measurement of probing depth, clinical attachment level, bleeding on probing, and evaluation of mobility using a periotest device. Additionally, information regarding the patient's smoking status, frequency of smoking, and oral hygiene habits was obtained.

Control group; Psoriasis patients

Eligibility Criteria

• Age: 18 Years - 80 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

The patients living in Eastern region of Turkey and visiting our clinic

You may qualify if:

• Patients previously diagnosed with psoriasis
• Adults who are 18 years of age or older
• Patients having at least 11 teeth

You may not qualify if:

• Patients with any known systemic disease other than psoriasis
• Patients who had received antibiotic, nonsteroidal anti-inflammatory, and/or immunomodulatory therapy within the last three months
• Patients who had received radiotherapy and/or chemotherapy within the last years

Sponsors & Collaborators

• Kahramanmaras Sutcu Imam University: lead

Study Sites (1)

Ayşe Gül Öner Talmaç

Kahramanmaraş, Onikişubat, 46000, Turkey (Türkiye)

Location

Biospecimen

Retention: SAMPLES WITH DNA

Gingival crevicular fluid

MeSH Terms

Conditions

Psoriasis; Periodontal Diseases

Condition Hierarchy (Ancestors)

Skin Diseases, Papulosquamous; Skin Diseases; Skin and Connective Tissue Diseases; Mouth Diseases; Stomatognathic Diseases

Study Officials

• Ayşe Gül Öner Talmaç, PhD

  Faculty of Dentistry, Kahraman Maraş Sütçü İmam University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: RETROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: assistant professor

Study Record Dates

• First Submitted: April 15, 2024
• First Posted: May 10, 2024
• Study Start: February 1, 2023
• Primary Completion: April 10, 2023
• Study Completion: August 15, 2023
• Last Updated: May 10, 2024

Record last verified: 2024-05

Data Sharing

• IPD Sharing: Will not share

Locations

TU (1)