Detection of Biofilm Among Uropathogenic Escherichia Coli Clinical Isolates in Suez Canal University Hospitals

NCT06252428 | Completed

• 1 other identifier: Moshera Ahmed Sherif Sherif
• Study Type: observational
• Target: 47
• Locations: 1 country
• Sites: 1

Brief Summary

This descriptive cross-sectional study will beconducted in Suez Canal University Hospitals (SCUHs) in Ismailia, Egypt. The study aims to detect Escherichia coli biofilm producers to improve prognosis and treatment and reduce morbidity and mortality rates due to this infection.

Conditions

Escherichia Coli Infections

Keywords

Uropathogenic E. coli; biofilm; antibiotic resistance; MTCP; CRA

Outcome Measures

Primary Outcomes (3)

• antibiotic resistance

  Susceptibility testing will be performed according to the standard Kirby-Bauer disk diffusion method on Mueller Hinton agar and interpreted according to the Clinical and Laboratory Standard Institute (CLSI) guidelines. Antibiotic discs include Amoxicillin-clavulanate, Piperacillin-tazobactam, Cefotaxime, Ceftazidime, Aztreonam, Imipenem, Gentamicin, Amikacin, Norfloxacin, ciprofloxacin, levofloxacin, Trimethoprim-sulfamethoxazole, Nitrofurantoin.

  from 18-24 hours

• Congo red Agar method (CRA)

  The medium will be prepared by adding 37 gm brain heart infusion (BHI) powder, 50 gm sucrose and 10 gm agar to 900 ml distilled water and autoclaved at 121°C for 15 minutes. Congo red will be prepared separately by dissolving 0.8 gm of congo red stain in 100 ml distilled water as concentrated aqueous solution and autoclaved at 121°C for 15 minutes and thenit will be added to the previous media when it cooled to 55°C. The congo red agar will be distributed in sterile plates. Plates will be inoculated by the test bacteria and incubated aerobically for 24 to 48 hours at 37°C. Positive results will be indicated by black colonies with a dry crystalline consistency. Weak slime producers usually remain pink.

  24 to 48 hours

• Modified tissue culture plate method (MTCP)

  All isolates will be screened for their ability to form biofilm by the TCP method with a modification in duration of incubation, which will be extended to 24 hours and the addition of glucose.

  up to 24 hours

Eligibility Criteria

• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

All patients of both sexes and all age groups attending different departments in Suez Canal University hospitals.

You may qualify if:

• Patients showing criteria for Symptomatic Urinary Tract Infection (SUTI) according to Center of Diseases and Control criteria (CDC) (CDC, 2022).
• Patient has at least one of the following signs or symptoms:
• fever (\>38.0°C)
• suprapubic tenderness
• costovertebral angle pain or tenderness
• urinary urgency
• urinary frequency
• dysuria

You may not qualify if:

• \. Patients received antibiotic treatment in the last 48 hours 2. Refusal of the patients to participate in the study

Sponsors & Collaborators

• Suez Canal University: lead

Study Sites (1)

Faculty of Medicine, Suez canal university

Ismailia, Egypt

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Urine specimens will be collected under aseptic precautions from clinically suspected patients with UTIs in different wards in Suez Canal University hospitals.

MeSH Terms

Conditions

Escherichia coli Infections

Condition Hierarchy (Ancestors)

Enterobacteriaceae Infections; Gram-Negative Bacterial Infections; Bacterial Infections; Bacterial Infections and Mycoses; Infections

Study Officials

• Rania Kishk, professor

  Faculty of Medicine - Suez Canal University

  STUDY CHAIR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: January 30, 2024
• First Posted: February 9, 2024
• Study Start: December 20, 2022
• Primary Completion: February 1, 2024
• Study Completion: February 20, 2024
• Last Updated: March 5, 2024

Record last verified: 2024-03

Locations

EG (1)