Asprosin, Adropin, Irisin Levels in Periodontitis

NCT06250764 | Completed

• 1 other identifier: 2020/9-10
• Study Type: observational
• Target: 122
• Locations: 1 country
• Sites: 1

Brief Summary

The recently discovered association of the hormones 'asprosin, adropin and irisin' with obesity and metabolic status, as well as the fact that periodontal diseases are affected by obesity and nutrition bilaterally, have led us to investigate the relationship between selected target markers and periodontal disease. The primary aim and main objective of this study is to increase the knowledge and to direct future researches as a result of the lack of adequate research in the past and the very limited investigations with these hormones in dentistry and periodontology. Our study was conducted on 122 patients who applied to Adıyaman University Faculty of Dentistry and were referred to the Department of Periodontology for routine periodontal controls. Participants' gender, age, height, weight, BMI, education level, tooth brushing and flossing habits as well as clinical parameters PI, GI, SCD, CAS and SDI were noted. Participants were divided into 4 groups as healthy, gingivitis, initial periodontitis and advanced periodontitis and grouped according to the current periodontal classification of 2017. DOS was collected from the groups for examination and 'asprosin, adropin and irisin' values in the samples were evaluated by ELISA test.

Conditions

Periodontal Diseases; Gingivitis; Obesity

Keywords

adropin; asprosin; irisin; periodontal disease; gingival crevicular fluid

Outcome Measures

Primary Outcomes (1)

• Association of PD and adipokine values

  correlation identification using SPSS between pocket depth and these adipokines in periodontitis patients

  only baseline measurement the patients were seen once

Study Arms (4)

GROUP 1 (periodontally healthy )

An individual to be considered to have periodontal health according to this definition, the percentage of sites with bleeding on probing should be no more than 10%, and the probing depth should be 3 mm or less

Diagnostic Test: Clinical periodontal measuremenets; Diagnostic Test: ELISA test from gingival crevicular fluid (GCF)

GROUP 2 (gingivitis)

Patients with gingivitis had PD ≤3 mm, BOP in ≥30% of the probing sites, and no interproximal AL or radiographic bone loss

Diagnostic Test: Clinical periodontal measuremenets; Diagnostic Test: ELISA test from gingival crevicular fluid (GCF)

GROUP 3(generalized stage I and stage II periodontitis)

Stage I periodontitis was diagnosed with interdental attachment loss (AL) ranging between 1 and 2 mm at the site of greatest loss. Radiographic bone loss (BL) is limited to less than 15% and no tooth loss. Additionally, the maximum probing depth (PD) is equal to or less than 4 mm or primarily involves horizontal bone loss. Stage II periodontitis was diagnosed with interdental attachment loss (AL) is ranging between 3 and 4 mm at the site of greatest loss. Radiographic bone loss (BL) extends to a range of 15% to 33%, there is no tooth loss. The maximum probing depth (PD) is equal to or less than 5 mm or primarily involves horizontal bone loss.

Diagnostic Test: Clinical periodontal measuremenets; Diagnostic Test: ELISA test from gingival crevicular fluid (GCF)

GROUP 4 (generalized stage III and stage IV periodontitis)

Stage III periodontitis is diagnosed with interdental attachment loss (AL) is ≥ 5, radiographic bone loss (BL) extends to mid-third of the root and beyond, tooth loss may become evident ≤4 teeth. Either PD ≥ 6 mm or vertical BL ≥ 3 mm, or Class II/III furcation involvement, or moderate ridge defect. Stage IV periodontitis is diagnosed with interdental attachment loss (AL) is ≥ 5 mm at the site of greatest loss, radiographic BL extending to mid-third of the root and beyond. ≥5 tooth loss due to periodontitis is evident. In addition to Stage III, either masticatory dysfunction, or secondary occlusal trauma (tooth mobility degree ≥ 2), or severe ridge defect, or bite collapse, or teeth drifting and flaring

Diagnostic Test: Clinical periodontal measuremenets; Diagnostic Test: ELISA test from gingival crevicular fluid (GCF)

Interventions

Clinical periodontal measuremenets DIAGNOSTIC_TEST

The periodontal assessment was performed by a single trained examiner (S.D.). A graded periodontal probe (Williams, Hu-Friedy, Chicago, IL, USA) was used to measure probing depth (PD) which was determined as the depth from the gingival margin to the bottom of the gingival sulcus/periodontal pocket, clinical attachment loss (CAL) which was calculated as the distance from the cemento-enamel junction to the bottom of the periodontal pocket and dual recording (+/-) of bleeding on probing (BOP), gingival index (GI) and plaque index (PI) in total except third molars G

GROUP 1 (periodontally healthy ); GROUP 2 (gingivitis); GROUP 3(generalized stage I and stage II periodontitis); GROUP 4 (generalized stage III and stage IV periodontitis)

ELISA test from gingival crevicular fluid (GCF) DIAGNOSTIC_TEST

GCF sampled from the buccal aspects of non-contiguous proximal regions in maxillary single-rooted teeth. In the periodontitis groups, fluid samples were taken from the two deepest pockets. In the gingivitis and periodontal healthy groups, fluid samples were obtained from areas with and without visible inflammation, respectively

GROUP 1 (periodontally healthy ); GROUP 2 (gingivitis); GROUP 3(generalized stage I and stage II periodontitis); GROUP 4 (generalized stage III and stage IV periodontitis)

Eligibility Criteria

• Age: 25 Years - 60 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

All patients admitted to the periodontology clinic between the study dates

You may not qualify if:

• Patients with cardiovascular disease, liver and kidney disease, metabolic disorders, diabetes, asthma, thyroid dysfunction, who used antibiotics or anti-inflammatory drugs within the last three months, who received periodontal treatment within the last six months, obese patients with body mass index (BMI) ≥ 30 kg/m2 according to the reference range determined by the World Health Organisation, and patients who refused to participate were excluded from the study

Sponsors & Collaborators

• AYSAN LEKTEMUR ALPAN: lead

Study Sites (1)

Adıyaman University Faculty of Dentistry

Adıyaman, Turkey (Türkiye)

Location

Related Publications (2)

• Loesche WJ, Grossman NS. Periodontal disease as a specific, albeit chronic, infection: diagnosis and treatment. Clin Microbiol Rev. 2001 Oct;14(4):727-52, table of contents. doi: 10.1128/CMR.14.4.727-752.2001.

  PMID: 11585783 BACKGROUND

• Torumtay Cin G, Fenkci SM, Kilic ID, Aslan HS, Sevgican CI, Senol H. The effects of severe periodontitis on arterial stiffness using cardio-ankle vascular index in patients with type 2 diabetes. J Periodontal Res. 2024 Feb;59(1):74-83. doi: 10.1111/jre.13202. Epub 2023 Nov 1.

  PMID: 37909328 BACKGROUND

MeSH Terms

Conditions

Periodontal Diseases; Gingivitis; Obesity; Arachnodactyly

Condition Hierarchy (Ancestors)

Mouth Diseases; Stomatognathic Diseases; Infections; Gingival Diseases; Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Limb Deformities, Congenital; Musculoskeletal Abnormalities; Musculoskeletal Diseases; Congenital Abnormalities; Congenital, Hereditary, and Neonatal Diseases and Abnormalities

Study Officials

• METİN ÇALIŞIR, PHD

  Adiyaman University

  PRINCIPAL INVESTIGATOR

• Sevde DEMİRCİ

  Adiyaman University

  STUDY DIRECTOR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Target Duration: 3 Days
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Associate Professor

Study Record Dates

• First Submitted: February 1, 2024
• First Posted: February 9, 2024
• Study Start: February 12, 2020
• Primary Completion: February 23, 2022
• Study Completion: February 23, 2022
• Last Updated: February 9, 2024

Record last verified: 2024-02

Locations

TU (1)