Endothelial NO Synthase Polymorphic Gene in Coronary Artery Dilation Disease

NCT06220682 | Completed

• 1 other identifier: Kasralainy school of medicine
• Study Type: observational
• Target: 100
• Locations: 1 country
• Sites: 1

Brief Summary

Introduction: The coronary artery dilatations disease (Coronary artery aneurysm/coronary artery ectasia) characterized by inappropriate coronary artery dilations. (c.894G\>T) is a gene polymorphism that may decreases eNOS enzyme activity which increases the risk for coronary heart disease by affecting released (NO) molecules. One of the most researched variations in eNOS gene is c.894G\>T polymorphism, which changes amino acid sequence at position 298 from Glu (GAG) to Asp (GAT), nevertheless, its role in Coronary dilatations disease still uncertain. Aim of study: To study correlation between the c.894G\>T gene polymorphism and angiographically dilated coronary artery (CAA/CAE), as well as to determine whether there is any association between this polymorphism and atherosclerotic CAD. Methodology: A comparative study on 100 patients with acute coronary syndrome who underwent coronary angiography from December 2020 to June 2022. According to standard angiographic views and expert opinions, patients were divided as, 25 patients normal, 25 patients had only atherosclerotic coronary artery disease, 25 patients had dilated coronaries (CAA/CAE) without atherosclerosis and 25 patients had dilated coronaries with atherosclerotic coronary artery disease. The specific genetic PCR laboratory test were performed for eNOS SNPs (single nucleotide polymorphism) namely, 894G \> T (Glu298Asp; rs 1799983), using Real-Time PCR.

Conditions

Coronary Artery Ectasia

Outcome Measures

Primary Outcomes (1)

• : To study correlation between the c.894G>T gene polymorphism and angiographically dilated coronary artery (CAA/CAE)

  : To study correlation between the c.894G\>T gene polymorphism and angiographically dilated coronary artery (CAA/CAE)

  1 year

Study Arms (4)

normal group

25 patients angiographically normal coronaries

Diagnostic Test: The specific genetic PCR laboratory test were performed for all selected patients for eNOS SNPs

atherosclerotic non dilated group

25 patients angiographically had atherosclerotic plaques with no coronary dilatations

dilated non atherosclerotic group

25 patients angiographically involved coronary dilatations with no atherosclerotic plaques

dilated atherosclerotic group

25 patients angiographically had both coronary dilatations and atherosclerotic plaques

Diagnostic Test: The specific genetic PCR laboratory test were performed for all selected patients for eNOS SNPs

Interventions

The specific genetic PCR laboratory test were performed for all selected patients for eNOS SNPs DIAGNOSTIC_TEST

using Real-Time PCR (QuantStudioTM 3 Real-Time PCR) with an allele-specific fluorogenic oligonucleotide probe and a TaqMan pre-designed SNP Genotyping assay

dilated atherosclerotic group; normal group

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

The total populations in our study were 100 patients, divided into 4 groups depends on angiographic results: Group (1): 25 patients angiographically normal coronaries (normal group). Group (2): 25 patients angiographically had atherosclerotic plaques with no coronary dilatations (atherosclerotic non dilated group). Group (3): 25 patients angiographically involved coronary dilatations with no atherosclerotic plaques (dilated non atherosclerotic group). Group (4): 25 patients angiographically had both coronary dilatations and atherosclerotic plaques (dilated atherosclerotic group).

You may qualify if:

• Patients who have angiographically coronary artery dilatations (coronary artery aneurysm or ectasia) (CAA/CAE) with or without angiographic evidence of atherosclerotic CAD.
• Another group of patients with angiographically normal or atherosclerotic coronaries without evidence of dilatation.

You may not qualify if:

• Patients with secondary coronary dilatations after balloon angioplasty, coronary stent implantation, patients with coronary artery bypass surgery who have post CABG native coronary dilatations, all were excluded from this study.

Sponsors & Collaborators

• Cairo University: lead

Study Sites (1)

Cairo university

Cairo, 11345, Egypt

Location

MeSH Terms

Conditions

Coronary Aneurysm

Condition Hierarchy (Ancestors)

Coronary Disease; Myocardial Ischemia; Heart Diseases; Cardiovascular Diseases; Aneurysm; Vascular Diseases

Study Officials

• Ahmed Mowafy, MD

  Cairo University

  STUDY CHAIR

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Target Duration: 2 Years
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Lecturer of critical care

Study Record Dates

• First Submitted: December 27, 2023
• First Posted: January 24, 2024
• Study Start: December 1, 2020
• Primary Completion: June 30, 2022
• Study Completion: June 30, 2022
• Last Updated: January 25, 2024

Record last verified: 2024-01

Data Sharing

• IPD Sharing: Will not share

Locations

EG (1)