Testosterone-induced Muscle Memory and Underlying Mechanisms
Long-term Effects of Anabolic Androgenic Steroids on Muscle Growth Potential: Testosterone-induced Muscle Memory and Underlying Mechanisms
1 other identifier
interventional
30
1 country
1
Brief Summary
The purpose of this project is to investigate underlying mechanisms of testosterone- and resistance training-induced muscle hypertrophy and muscle memory in humans.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at below P25 for not_applicable
Started Apr 2025
Longer than P75 for not_applicable
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
First Submitted
Initial submission to the registry
December 18, 2023
CompletedFirst Posted
Study publicly available on registry
January 3, 2024
CompletedStudy Start
First participant enrolled
April 1, 2025
CompletedPrimary Completion
Last participant's last visit for primary outcome
December 1, 2027
ExpectedStudy Completion
Last participant's last visit for all outcomes
December 1, 2028
April 10, 2025
April 1, 2025
2.7 years
December 18, 2023
April 7, 2025
Conditions
Outcome Measures
Primary Outcomes (3)
Fiber cross-sectional area
Immunohistochemistry to determine fiber cross-sectional area
The change in fiber cross-sectional is assessed at baseline, 4, 20 and 24 weeks
Myonuclei
Immunohistochemistry to determine number of myonuclei
The change in myonuclei is assessed at baseline, 4, 20 and 24 weeks
Skeletal muscle proteome
Pathway enrichment analysis of the muscle proteome using mass spectrometry-based proteomics
The change in the muscle proteome is assessed at baseline, 4, 20 and 24 weeks
Secondary Outcomes (3)
Lean mass
The change in lean mass is assessed at baseline, 4, 20 and 24 weeks
Satellite cells
The change in satellite cells is assessed at baseline, 4, 20 and 24 weeks
Muscle strength
The change in 1-repetition maximum is assessed at baseline, 4, 20 and 24 weeks
Study Arms (2)
Testosterone
EXPERIMENTALParticipants are randomized to a single injection of 1000 mg testosterone undecanoate
Placebo
PLACEBO COMPARATORParticipants are randomized to a single placebo injection
Interventions
Following testosterone/placebo injection, participants undergo supervised resistance training for 4 weeks. This period is followed by a washout period with no drug and either continued resistance training or detraining for 16 weeks. Thereafter, another 4-week period with supervised resistance training is commenced, with no drug.
Eligibility Criteria
You may qualify if:
- Healthy men and women
- BMI of \<26 and normal ECG and blood pressure
You may not qualify if:
- Smoking
- Chronic disease,
- Use of prescription medication
- Pain due to current or previous musculoskeletal injury
- Resistance training more than once per week in the 12 months leading up to the intervention
- Current or previous use of prohibited anabolic substances
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Study Sites (1)
The August Krogh Section for Human and Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen
Copenhagen, 2100, Denmark
MeSH Terms
Interventions
Central Study Contacts
Study Design
- Study Type
- interventional
- Phase
- not applicable
- Allocation
- RANDOMIZED
- Masking
- QUADRUPLE
- Who Masked
- PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
- Purpose
- BASIC SCIENCE
- Intervention Model
- PARALLEL
- Sponsor Type
- OTHER
- Responsible Party
- SPONSOR INVESTIGATOR
- PI Title
- Associate Professor
Study Record Dates
First Submitted
December 18, 2023
First Posted
January 3, 2024
Study Start
April 1, 2025
Primary Completion (Estimated)
December 1, 2027
Study Completion (Estimated)
December 1, 2028
Last Updated
April 10, 2025
Record last verified: 2025-04