Periodontal Inflammation and Wound Healing in Multiple Extractions

NCT06185114 | Withdrawn

• 1 other identifier: 0886-23-EP
• Study Type: observational
• Target: N/A
• Locations: 0 countries
• Sites: N/A

Brief Summary

Four participants requiring multiple dental extractions will be recruited for this study. Two participants will be 21-50 years old, two participants will be 65-80 years old. Following tooth extractions, soft tissue sampling will be completed from the extraction sockets at baseline, one week, two weeks and three weeks post-extraction. Soft tissue will be processed for sc-RNA sequencing and/or flow cytometry to determine what cells, genomic pathways are present at various timepoints during wound healing following dental extractions.

Conditions

Wound Heal

Outcome Measures

Primary Outcomes (1)

• Inflammatory Cells

  Presence or absence of inflammatory cells

  Baseline, 1 week, 2 weeks, 3 weeks

Study Arms (2)

Young (ages 21-50) requiring multiple extractions

Participants aged 21-50 requiring multiple extractions will be consented. These participants will undergo soft tissue, gingival crevicular fluid (GCF), venous blood sampling and cone beam commuted tomography (CBCT) analysis to determine age-related factors associated with post-extraction wound healing.

Old (ages 65-80) requiring multiple extractions

Participants aged 65-80 requiring multiple extractions will be consented. The older participants will undergo soft tissue, gingival crevicular fluid (GCF), venous blood sampling and cone beam commuted tomography (CBCT) analysis to determine age-related factors associated with post-extraction wound healing.

Eligibility Criteria

• Age: 21 Years - 80 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Four participants seeking multiple dental extractions; 21-50 years old, 65-80 years old.

You may qualify if:

• requires multiple dental extractions
• aged 21-50, 65-80 years old

You may not qualify if:

• history of systemic inflammatory diseases or medications

Sponsors & Collaborators

• University of Nebraska: lead

Biospecimen

Retention: SAMPLES WITH DNA

Samples will be taken and retained for either scRNA sequencing and/or flow cytometry.

Study Officials

• Amy C Killeen, DDS, MS

  University of Nebraska

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: December 11, 2023
• First Posted: December 29, 2023
• Study Start: October 1, 2024
• Primary Completion: October 1, 2024
• Study Completion: October 1, 2024
• Last Updated: March 17, 2025

Record last verified: 2025-03

Data Sharing

• IPD Sharing: Will not share