Capture of Healthy Gut Bacteria Associated to High Fiber Diet

NCT06166810 | Recruiting

• 1 other identifier: 2023-A01677-38
• Study Type: observational
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

The role of the intestinal microbiota on health is now well recognised, and diet is one of the factors influencing the maintenance of a healthy intestinal microbiota. More specifically, the consumption of fruit, vegetables and cereals is associated with good health and, in particular, with an increase in the relative abundance of bacterial groups described as beneficial. To date, a large number of bacterial species in the intestinal microbiota have yet to be isolated and cultivated, which limits the characterisation of their potential health benefits. In order to isolate and cultivate these intestinal bacteria, it is particularly relevant to recruit individuals with a high-fibre diet.

Conditions

General Population

Outcome Measures

Primary Outcomes (1)

• Isolation and culture of one isolate from each target species

  Successful isolation and culture of at least one isolate from each target species previously identified through metagenomic data analyses.

  Up to 3 years

Secondary Outcomes (4)

• metagenomic profiles in relation to dietary profiles

  Up to 3 years

• growth capacity of the isolates

  Up to 3 years

• Functional potential of the isolates

  up to 3 years.

• Phenotypic caracterization of the isolates

  Up to 3 years

Eligibility Criteria

• Age: 20 Years - 50 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

General population residing in Clermont-Ferrand (France)

You may qualify if:

• Healthy adult subjects ;
• Age between 20 and 50 (limits included) ;
• BMI between 18.5 and 25 kg/m2 (excluding limits) ;
• Consumption of more than 5 portions of fruit and vegetables a day;
• Intake of at least 25g of dietary fibre per day;
• A varied diet with little processed food.

You may not qualify if:

• Antibiotics and/or transit modulators taken in the 3 months prior to sampling;
• Known pathologies;
• Current treatment;
• History of digestive surgery having an impact on the microbiota, deemed incompatible with the study (bariatric surgery);
• Consumption of food supplements based on protein preparations, probiotics or prebiotics;
• Smoking (even occasionally);
• Alcohol consumption \> 2 glasses (i.e. 20 g) per day;
• Intense sporting activity;
• Pregnant or breast-feeding women;
• Persons under legal protection (curatorship, guardianship, etc.);
• Volunteers taking part in another intervention study;
• Persons refusing to be registered on the French Ministry of Health's Fichier National des Volontaires Sains (National Register of Healthy Volunteers);
• Subject not affiliated to the social security system;

Sponsors & Collaborators

• Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement: lead
• Centre de Recherche en Nutrition Humaine d'Auvergne: collaborator

Study Sites (1)

CRNH Auvergne

Clermont-Ferrand, 63009, France

RECRUITING

Related Publications (1)

• Caille A, Connan C, Lyon Belgy N, Borezee E, Cherbuy C, Meunier N, Meslier V. Positive nutritional selection of adults with healthy lifestyle and high daily fiber consumption for the isolation of beneficial intestinal bacteria: The iTARGET cohort study protocol. MethodsX. 2025 Mar 17;14:103268. doi: 10.1016/j.mex.2025.103268. eCollection 2025 Jun.

  PMID: 40224142 DERIVED

Biospecimen

Retention: SAMPLES WITH DNA

Fecal samples

Study Officials

• Victoria MESLIER, PhD

  Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

  STUDY DIRECTOR

• Claire CHERBUY, PhD

  Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

  STUDY DIRECTOR

Central Study Contacts

Aurélie CAILLE, MSc

CONTACT

04 73 75 42 73; acaille@chu-clermontferrand.fr

Nathalie MEUNIER, PhD

CONTACT

04 73 75 42 73; nmeunier@chu-clermontferrand.fr

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Target Duration: 24 Months
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: December 4, 2023
• First Posted: December 12, 2023
• Study Start: October 24, 2023
• Primary Completion: December 31, 2025
• Study Completion: January 31, 2026
• Last Updated: January 15, 2026

Record last verified: 2025-09

Data Sharing

• IPD Sharing: Will not share

Locations

FR (1)