Influence of Hypoxic, Normobaric and Hypobaric Training on the Immunometabolism of Post-covid-19 Athletes

NCT06128941 | Recruiting

• 1 other identifier: SprintRepHipoxya
• Study Type: interventional
• Target: 60
• Locations: 1 country
• Sites: 1

Brief Summary

COVID-19 has significantly impacted sports globally, with event postponements, training disruptions, and wide-ranging concerns. SARS-CoV-2 infection can result in hyperinflammation and cardiopulmonary changes, with hypoxia as an aggravating sign. Hypoxia triggers complex immunometabolic mechanisms, including activation of HIF-1α and induction of HLA-G expression. Hypoxia training protocols benefit aerobic capacity and sports performance, with potential immunological impact. Studying immunometabolic markers in this context can improve athletic preparation and athletes' general health.

Conditions

Normobaric Hypoxia; Hypoventilation; Normoxia

Keywords

HIF1a; HLA-G; Repeated sprint; Inflammation; covid-19; immune system; athletes

Outcome Measures

Primary Outcomes (9)

• Hypoxia Inducible Factor 1 alpha (HIF-1a)

  Flow cytometry for PBMCs

  Before, just after and 4 weeks after the training program

• Human Leukocyte Antigen-G (HLA-G)

  ELISA

  Before, just after and 4 weeks after the training program

• Cytokines (TNF-α, IL-1β, IL-6, IL-10, IL-8 and IFN-γ)

  ELISA

  Before, just after and 4 weeks after the training program

• Plasma levels of eicosanoids, endocannabinoids, steroid hormones, sphingolipids, ceramides and other glycerophospholipids

  Mass Spectrometry (LC-MS/MS)

  Before, just after and 4 weeks after the training program

• Hematological indicators (hematocrit, hemoglobin and cell count)

  Blood count

  Before, just after and 4 weeks after the training program

• Ventilatory thresholds and maximum oxygen consumption

  mL/kg·min

  Before, just after and 4 weeks after the training program

• Blood lactate concentrations

  mmol.L-1

  Before, just after and 4 weeks after the training program

• Muscle oximetry

  Near-infrared spectroscopy (NIRS)

  Before, just after and 4 weeks after the training program

• Peak force

  Isometric mid thigh pull

  Before, just after and 4 weeks after the training program

Secondary Outcomes (5)

• Heart rate

  Throughout the training program, 4 weeks

• Arterial oxygen saturation

  Throughout the training program, 4 weeks

• Subjective perception of effort

  Throughout the training program, 4 weeks

• Blood pressure, heart rate and temperature

  Before, just after and 4 weeks after the training program

• Anthropometric assessments and body composition

  Before, just after and 4 weeks after the training program

Study Arms (3)

Normobaric hypoxia (SRH)

EXPERIMENTAL

Participants will carry out training sessions in a normobaric hypoxia chamber at a simulated altitude of 3000 meters (FiO2 14.5%)

Other: Repeated sprint

Hypoventilation (SRH-VLH)

EXPERIMENTAL

Participants will be asked to exhale to residual functional capacity, immediately before starting each sprint, and to hold their breath until the end of the sprint

Other: Repeated sprint

Normoxia (SRN)

ACTIVE COMPARATOR

Participants will carry out training sessions in normoxia (FiO2 20.9%)

Other: Repeated sprint

Interventions

Repeated sprint OTHER

* Duration of the study: 8 weeks of participation. * Each repeated sprint training protocol: 2 training sessions per week for 4 weeks. * Collection sessions: 3 collection sessions will be carried out: before the start of the training protocol - T0; at the end of the protocol, in week 4 - T1; and late, 4 weeks after the end of the protocol, in week 8 - T2. * Training sessions: will be carried out on an ergometer and will consist of 3 sets of 5 sprints of 10s all-out with 20s of rest between sprints, and 5 minutes of rest between sets.

Hypoventilation (SRH-VLH); Normobaric hypoxia (SRH); Normoxia (SRN)

Eligibility Criteria

• Age: 18 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• more than 5 years of training experience in an resistance modality;
• participates in national or international championships regularly;
• athletes convalescing from covid-19, at least 30 days after diagnosis and/or hospital discharge;
• manifested mild to severe symptoms;
• vaccinated or not against SARS-CoV-2;
• antigen self-test for SARS-CoV-2 negative.

You may not qualify if:

• athletes who have had an acclimatization experience or exposure to altitude lasting more than 10 days in the last 6 months;
• contain signs or symptoms of acute covid-19;
• present a positive SARS-CoV-2 antigen self-test;
• pregnant or postpartum women;
• use anti-inflammatory or immunosuppressive medications.

Sponsors & Collaborators

• Faculdade de Motricidade Humana: lead
• University of Sao Paulo: collaborator
• Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.: collaborator

Study Sites (1)

Faculty of Human Kinetics

Lisbon, 1495-002 Cruz-Quebrada, Portugal

RECRUITING

MeSH Terms

Conditions

Hypoventilation; Inflammation; COVID-19

Condition Hierarchy (Ancestors)

Respiratory Insufficiency; Respiration Disorders; Respiratory Tract Diseases; Signs and Symptoms, Respiratory; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Pathologic Processes; Pneumonia, Viral; Pneumonia; Respiratory Tract Infections; Infections; Virus Diseases; Coronavirus Infections; Coronaviridae Infections; Nidovirales Infections; RNA Virus Infections; Lung Diseases

Central Study Contacts

Cristina Monteiro, PhD

CONTACT

00351214149174; cmonteiro@fmh.ulisboa.pt

Joana Reis, PhD

CONTACT

00351214149174; joanareis@fmh.ulisboa.pt

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant professor

Study Record Dates

• First Submitted: November 9, 2023
• First Posted: November 13, 2023
• Study Start: January 3, 2024
• Primary Completion: April 1, 2024
• Study Completion: June 1, 2024
• Last Updated: February 22, 2024

Record last verified: 2024-02

Data Sharing

• IPD Sharing: Will not share

Locations

PT (1)