Dileucine and Resistance Training Adaptations

NCT06121869 | Completed

• 1 other identifier: IRB-41-64
• Study Type: interventional
• Target: 34
• Locations: 1 country
• Sites: 1

Brief Summary

This study seeks to compare the observed changes in resistance training adaptations after supplementation of isomolar amounts of leucine or dileucine in healthy resistance-trained men. This will provide a better understanding of supplementation with leucine versus dileucine over a 10-week period in regards to increasing muscular performance.

Conditions

Hypertrophy; Strength Training Adaptations; Weight-Bearing Strengthening Program

Keywords

Peptides; Dipeptide; Adaptations; Exercise; Dietary Supplements

Outcome Measures

Primary Outcomes (4)

• Fat-Free Mass

  Fat-free mass will be determined via Dual-Energy X-Ray Absorptiometry

  10 weeks

• Leg Press 1RM

  Leg Press 1RM will be measured using the leg press (lower-body) exercise.

  10 weeks

• Bench Press 1RM

  Bench Press 1RM will be measured using the bench press (upper-body) exercise.

  10 weeks

• Maximal Isometric Mid-Thigh Pull Force Production

  Maximal Isometric Mid-Thigh Pull Force Production will be measured using an isometric mid-thigh pull test

  10 weeks

Secondary Outcomes (13)

• Fat Mass

  10 weeks

• Lean mass

  10 weeks

• % Body Fat

  10 weeks

• Total body water

  10 weeks

• Extracellular Body Water

  10 weeks

Study Arms (3)

Placebo

PLACEBO COMPARATOR

Participants will supplement daily for 10 weeks with 2 grams rice flower placebo in capsule form. On training days, participants will consume their assigned supplement within 60 minutes of completing their workout. On non-training days, participants will consume their assigned supplement with their first meal of the day. All doses will be consumed with 8-12 ounces of water.

Dietary Supplement: Placebo

Leucine

ACTIVE COMPARATOR

Participants will supplement daily for 10 weeks with 2 grams rice leucine in capsule form. On training days, participants will consume their assigned supplement within 60 minutes of completing their workout. On non-training days, participants will consume their assigned supplement with their first meal of the day. All doses will be consumed with 8-12 ounces of water.

Dietary Supplement: Leucine

Dileucine

EXPERIMENTAL

Participants will supplement daily for 10 weeks with 2 grams dileucine (RAMPS, Ingenious Ingredients, TX, USA) in capsule form. On training days, participants will consume their assigned supplement within 60 minutes of completing their workout. On non-training days, participants will consume their assigned supplement with their first meal of the day. All doses will be consumed with 8-12 ounces of water.

Dietary Supplement: Dileucine

Interventions

Placebo DIETARY_SUPPLEMENT

Participants will supplement daily for 10 weeks with 2 grams rice flower placebo in capsule form. On training days, participants will consume their assigned supplement within 60 minutes of completing their workout. On non-training days, participants will consume their assigned supplement with their first meal of the day. All doses will be consumed with 8-12 ounces of water.

Placebo

Leucine DIETARY_SUPPLEMENT

Participants will supplement daily for 10 weeks with 2 grams leucine in capsule form. On training days, participants will consume their assigned supplement within 60 minutes of completing their workout. On non-training days, participants will consume their assigned supplement with their first meal of the day. All doses will be consumed with 8-12 ounces of water.

Leucine

Dileucine DIETARY_SUPPLEMENT

Participants will supplement daily for 10 weeks with 2 grams dileucine (RAMPS, Ingenious Ingredients, TX, USA) in capsule form. On training days, participants will consume their assigned supplement within 60 minutes of completing their workout. On non-training days, participants will consume their assigned supplement with their first meal of the day. All doses will be consumed with 8-12 ounces of water.

Dileucine

Eligibility Criteria

• Age: 18 Years - 39 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Male
• Between the ages of 18-39 years of age
• Currently participating in resistance training exercise

You may not qualify if:

• Female
• Participants who are determined to not be weight stable defined as week 0 and week 2 body mass levels deviating by 3% or more.
• Any individual who is currently being treated for or diagnosed with a cardiac, respiratory, circulatory, musculoskeletal, metabolic, immune, autoimmune, psychiatric, hematological, neurological or endocrinological disorder or disease.
• Body mass index \> 25 kg/m2. Individuals with a body mass index greater than 25 kg/m2, but a body fat percentage less than 25% fat will be accepted into the study
• Individuals who present with any previous injury or illness that would prevent them from appropriately completing all exercise lifts.
• Participants who are not able to fit onto the DEXA table will not be able to participate in the research due to size restrictions of the equipment. This typically includes individuals exceeding 400 pounds or those greater than 6'4".
• Those individuals with less than 12 months of structured resistance training experience will be excluded from the study.
• Individuals whose maximal relative upper body strength is below 1.0 will be excluded.
• Individuals whose maximal relative lower body strength is below 1.5 will be excluded.
• All participants who DO NOT abstain from taking any additional forms of nutritional supplementation deemed to be ergogenic or that will impact resistance training adaptations (pre-workouts, creatine, beta-alanine, etc.) for four weeks prior to beginning this study and for the entire duration of the study.
• Individuals who are currently using or have used within the past 12 months anabolic-androgenic steroids.

Sponsors & Collaborators

• Lindenwood University: lead
• Ingenious Ingredients, L.P.: collaborator

Study Sites (1)

Exercise and Performance Nutrition Laboratory

Saint Charles, Missouri, 63301, United States

Location

Related Publications (1)

• Hagele AM, Krieger JM, Gaige CJ, Holley KF, Gross KN, Iannotti JM, Allen LE, Sutton PJ, Orr LS, Mumford PW, Purpura M, Jager R, Kerksick CM. Dileucine ingestion, but not leucine, increases lower body strength and performance following resistance training: A double-blind, randomized, placebo-controlled trial. PLoS One. 2024 Dec 31;19(12):e0312997. doi: 10.1371/journal.pone.0312997. eCollection 2024.

  PMID: 39739679 DERIVED

MeSH Terms

Conditions

Hypertrophy; Motor Activity

Interventions

Leucine

Condition Hierarchy (Ancestors)

Pathological Conditions, Anatomical; Pathological Conditions, Signs and Symptoms; Behavior

Intervention Hierarchy (Ancestors)

Amino Acids, Branched-Chain; Amino Acids; Amino Acids, Peptides, and Proteins; Amino Acids, Essential

Study Officials

• Chad M Kerksick, PhD

  Lindenwood University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Masking Details: All supplements will be provided in blinded, labeled containers prior to them arriving to the site. Each supplement will be of similar volume, texture, and labeled in indistinguishable bottles to ensure blinding of both participant and invesitigator
• Purpose: OTHER
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: Randomized, Double-Blind, Placebo-Controlled

Study Record Dates

• First Submitted: November 2, 2023
• First Posted: November 8, 2023
• Study Start: May 7, 2021
• Primary Completion: June 29, 2023
• Study Completion: June 29, 2023
• Last Updated: November 8, 2023

Record last verified: 2023-11

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)