Modulation of the Gut Microbiota of Professional Athletes With Sauerkraut Supplementation

NCT06087146 | Completed

• 1 other identifier: KOMS77
• Study Type: interventional
• Target: 10
• Locations: 1 country
• Sites: 1

Brief Summary

Literature on the topic of athlete gut microbiota is scarce, and even more on the topic of its modulation. To address these issues a prospective cohort study was conceived. The cohort included young, male generally healthy professional athletes. The cohort will first be exposed to a course of sauerkraut supplementation as a synbiotic. Then the cohort will be observed without any intention for gut microbiota modulation. Before and after each study phase a gut microbiota analysis will be performed for its parameters. The aim of this study is to assess whether the intervention leads to significant changes in the gut microbiota parameters in all subjects and if yes what the signal of the intervention was, meaning whether the changes were consistent among all subjects regarding the analyzed parameter and its direction of effect. The results will be compared with data from earlier research on gut microbiota modulation in professional athletes with soluble fiber as prebiotics and a placebo. The study will provide valuable data on the athlete gut microbiota and its potential modulation.

Conditions

Gastrointestinal Microbiome

Keywords

Microbiota; Sports; Athletes; Nutrition Surveys; Probiotics

Outcome Measures

Primary Outcomes (1)

• Current

  -short term gut microbiota change- The impact of sauerkraut on gut microbiome shall assessed through monitoring of relative taxonomic abundance of bacteria that compose gut microbiota from samples taken day before the intervention and immediately after the intervention. The composition of the intestinal microbiota will be determined by sequencing the 16s rRNA bacteria in the stool sample. Subjects take a sample with a sterile cotton swab by wiping stool with toilet paper in their home on a given day research. The sample is stored in a tube with a buffer liquid so that the sample can be stored and transported at room temperature temperature. The test sample is transported by air to the laboratory of Biomes NGS Gesellschaft mit beschränkter Haftung, Wildau, Germany. The laboratory performs independently

  one month

Secondary Outcomes (1)

• Future

  three months

Study Arms (1)

Athletes supplemented with sauerkraut

EXPERIMENTAL

10 professional athletes (9 male) who will during 10 days consume approximately 250 g of sauerkraut (Brassica oleracea v. capitata) daily.

Other: Sauerkraut (Brassica oleracea v. capitata)

Interventions

Sauerkraut (Brassica oleracea v. capitata) OTHER

The research material is a donation from the company Eko Bio Zrnje, Vrbovec, Croatia. It's grated pickle cabbage produced by fermentation in a saline solution (NaCl) in which the fermentation was stopped by pasteurization and was packed in glass 500g jars. The nutritional and microbiological composition was tested by the laboratories of the Faculty of Food and Biotechnology, as well as by the cultural laboratory approach confirmed the presence of bacteria that produce lactic acid (4.82·103 (±2.31) CFU/mL)

Athletes supplemented with sauerkraut

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• regularly engaging in professional sports activities
• categorized athlete by HOO
• playing sports as a source of income (bodybuilding)
• older than 18 years old
• general general state of health
• written understanding of the English language

You may not qualify if:

• use of antibiotics for a period of at least six months before participating in the study application of nutritional supplements with the potential to modulate intestinal microbiota (probiotics, prebiotics, postbiotics) in the period from
• at least six months before participating in the study
• chronic drug therapy
• intolerance of one of the interventions (sauerkraut, fasting, probiotics and their additives)
• chronic medical therapy (except for vitamins, thyroid hormones and antihistamines)
• kidney function disorder
• liver function disorder
• history of digestive problems at the time before the study (diarrhea, dyspepsia, constipation)
• hormonal disorder (except hypothyroidism)
• psychological or mental disorder
• involvement in other research

Sponsors & Collaborators

• The Gut Microbiome Center (Centar za crijevni mikrobiom): lead
• University of Zagreb: collaborator

Study Sites (1)

Gut Microbiome Center

Zagreb, 10000, Croatia

Location

Related Publications (9)

• Nichols AW. Probiotics and athletic performance: a systematic review. Curr Sports Med Rep. 2007 Jul;6(4):269-73.

  PMID: 17618005 RESULT

• Jager R, Mohr AE, Carpenter KC, Kerksick CM, Purpura M, Moussa A, Townsend JR, Lamprecht M, West NP, Black K, Gleeson M, Pyne DB, Wells SD, Arent SM, Smith-Ryan AE, Kreider RB, Campbell BI, Bannock L, Scheiman J, Wissent CJ, Pane M, Kalman DS, Pugh JN, Ter Haar JA, Antonio J. International Society of Sports Nutrition Position Stand: Probiotics. J Int Soc Sports Nutr. 2019 Dec 21;16(1):62. doi: 10.1186/s12970-019-0329-0.

  PMID: 31864419 RESULT

• Toohey JC, Townsend JR, Johnson SB, Toy AM, Vantrease WC, Bender D, Crimi CC, Stowers KL, Ruiz MD, VanDusseldorp TA, Feito Y, Mangine GT. Effects of Probiotic (Bacillus subtilis) Supplementation During Offseason Resistance Training in Female Division I Athletes. J Strength Cond Res. 2020 Nov;34(11):3173-3181. doi: 10.1519/JSC.0000000000002675.

  PMID: 33105368 RESULT

• Mohr AE, Pugh J, O'Sullivan O, Black K, Townsend JR, Pyne DB, Wardenaar FC, West NP, Whisner CM, McFarland LV. Best Practices for Probiotic Research in Athletic and Physically Active Populations: Guidance for Future Randomized Controlled Trials. Front Nutr. 2022 Mar 8;9:809983. doi: 10.3389/fnut.2022.809983. eCollection 2022.

  PMID: 35350412 RESULT

• Marco ML, Hutkins R, Hill C, Fulgoni VL, Cifelli CJ, Gahche J, Slavin JL, Merenstein D, Tancredi DJ, Sanders ME. A Classification System for Defining and Estimating Dietary Intake of Live Microbes in US Adults and Children. J Nutr. 2022 Jul 6;152(7):1729-1736. doi: 10.1093/jn/nxac074.

  PMID: 35583208 RESULT

• Dimidi E, Cox SR, Rossi M, Whelan K. Fermented Foods: Definitions and Characteristics, Impact on the Gut Microbiota and Effects on Gastrointestinal Health and Disease. Nutrients. 2019 Aug 5;11(8):1806. doi: 10.3390/nu11081806.

  PMID: 31387262 RESULT

• Touret T, Oliveira M, Semedo-Lemsaddek T. Putative probiotic lactic acid bacteria isolated from sauerkraut fermentations. PLoS One. 2018 Sep 7;13(9):e0203501. doi: 10.1371/journal.pone.0203501. eCollection 2018.

  PMID: 30192827 RESULT

• Costa MS, Toscano LT, Toscano LLT, Luna VR, Torres RA, Silva JA, Silva AS. Ergogenic potential of foods for performance and recovery: a new alternative in sports supplementation? A systematic review. Crit Rev Food Sci Nutr. 2022;62(6):1480-1501. doi: 10.1080/10408398.2020.1844137. Epub 2020 Nov 23.

  PMID: 33226268 RESULT

• Hughes RL, Holscher HD. Fueling Gut Microbes: A Review of the Interaction between Diet, Exercise, and the Gut Microbiota in Athletes. Adv Nutr. 2021 Dec 1;12(6):2190-2215. doi: 10.1093/advances/nmab077.

  PMID: 34229348 RESULT

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: OTHER
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: October 11, 2023
• First Posted: October 17, 2023
• Study Start: October 31, 2023
• Primary Completion: November 13, 2023
• Study Completion: November 29, 2023
• Last Updated: December 5, 2023

Record last verified: 2023-11

Locations

CR (1)