NCT06070961

Brief Summary

This is a prospective biological study evaluating the persistence of COVID-19 vaccine and other vaccines' (zoster, diphtheria and tetanus)-induced immunity in a subgroup patient affected by Follicular Lymphoma requiring treatment undergoing frontline induction immuno-chemotherapy and anti-CD20 maintenance within the prospective FIL\_FOLL19 study (NCT05058404). Blood samples from patients will be collected before and at planned timepoints during treatment to evaluate humoral and cellular immunity against SARS-COV-2, VZV, tetanus and diphtheria and T-cell markers characterization.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
56

participants targeted

Target at P25-P50 for all trials

Timeline
12mo left

Started May 2024

Typical duration for all trials

Geographic Reach
1 country

14 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress67%
May 2024May 2027

First Submitted

Initial submission to the registry

October 3, 2023

Completed
3 days until next milestone

First Posted

Study publicly available on registry

October 6, 2023

Completed
7 months until next milestone

Study Start

First participant enrolled

May 7, 2024

Completed
3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 1, 2027

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

May 1, 2027

Last Updated

June 29, 2025

Status Verified

June 1, 2025

Enrollment Period

3 years

First QC Date

October 3, 2023

Last Update Submit

June 25, 2025

Conditions

Keywords

LymphomaFollicularImmunochemotherapyFLVaccineCOVID19SARS-COV-2TetanusZosterVZVRituximabobinotuzumabFOLL19FIL_FOLL19diphtheriaMaintenanceHumoralCellularImmunityInduction

Outcome Measures

Primary Outcomes (1)

  • Rate of patients with persistence of cell-mediated immunity induced by COVID-19 approved vaccines (at least three doses) after standard induction immuno-chemotherapy.

    Proportion of patients with laboratory parameters of vaccine-induced cellular immunity against SARS-CoV-2 (by ELISpot assay) at EOI (after induction immuno-chemotherapy) vs proportion at baseline

    At the end of induction therapy (EOI) - About 8 months from treatment start

Secondary Outcomes (7)

  • Rate of patients with persistence of cell-mediated immunity induced by COVID-19 approved vaccines (at least three doses) during anti-CD20 mAbs maintenance

    At +12 months of maintenance - About 20 months from treatment start

  • Rate of patients with persistence of humoral immunity induced by COVID-19 approved vaccines (at least three doses) after standard induction immuno-chemotherapy and during maintenance.

    At the end of induction therapy (EOI) and at +12 months of maintenance - About 8 months and 20 months from treatment start respectively

  • Rate of patients (with a detectable serologic response at study entry) with persistence of humoral and cellular immunity induced by adjuvanted recombinant zoster vaccine after standard induction immuno-chemotherapy and during maintenance

    At the end of induction therapy (EOI) and at +12 months of maintenance - About 8 months and 20 months from treatment start respectively

  • Rate of patients (with a detectable serologic response at study entry) with persistence of humoral immunity induced by childhood vaccines (diphtheria and tetanus) after standard induction immuno-chemotherapy and during maintenance

    At the end of induction therapy (EOI) and at +12 months of maintenance - About 8 months and 20 months from treatment start respectively

  • Rate of COVID-19 infection events and severity in vaccinated patients and correlation with humoral and/or cellular immunity with eventual tixagevimab/cilgavimab or other MAb prophylaxis and with dominant SARS-CoV-2 variant/subvariant at time of infection

    From baseline (before therapy) up to 20 months from treatment start (+12 months of maintenance)

  • +2 more secondary outcomes

Study Arms (1)

Patients enrolled

Patient affected by advanced Follicular Lymphoma undergoing front-line immunochemotherapy and antiCD-20 maintenance in the FIL\_FOLL19 trial

Diagnostic Test: Cellular immunity vs SARS-CoV-2Diagnostic Test: Humoral immunity vs SARS-CoV-2Diagnostic Test: Cellular immunity vs Varicella Zoster VirusDiagnostic Test: Humoral immunity vs Varicella Zoster VirusDiagnostic Test: Diphtheria toxin-binding IgGDiagnostic Test: Tetanus toxoid-binding IgGDiagnostic Test: T-cell populations and markers characterization

Interventions

Evaluation of cellular immunity vs SARS-CoV-2 by ELISpot assay

Patients enrolled

Evaluation of Humoral immunity vs SARS-CoV-2 by ELISA assay (IgG anti-RBD and anti-N)

Patients enrolled

Evaluation of cellular immunity vs VZV by Enzyme-Linked immunoSPOT (ELISPOT) assay

Patients enrolled

Evaluation of humoral immunity vs VZV by ELISA (VZV gE-binding IgG)

Patients enrolled

Evaluation of diphtheria toxin-binding IgG by ELISA assay

Patients enrolled

Evaluation of tetanus toxoid-binding IgG by ELISA assay

Patients enrolled

Characterization of T-cell populations and markers by flow cytometry

Patients enrolled

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

Patients affected by Follicular Lymphoma undergoing frontline induction immuno-chemotherapy and anti-CD20 maintenance within the prospective FIL\_FOLL19 study

You may qualify if:

  • Enrolment in FIL\_FOLL19 study
  • Previous vaccination for COVID-19 (at least 3 doses)
  • Availability of informations about COVID-19 and other vaccines previously administered (vaccination records)
  • Willingness to comply with blood collection timepoints required for vaccination immunity evaluation
  • Signature of specific informed consent form

You may not qualify if:

  • None

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (14)

Azienda Ospedaliera S.Giuseppe Moscati - S.C. Ematologia e Trapianto emopoietico

Avellino, AV, 83100, Italy

RECRUITING

A.O. SS. Antonio e Biagio e Cesare Arrigo, S.C. Ematologia

Alessandria, IT, Italy

RECRUITING

Nuovo Ospedale degli Infermi, SSD Ematologia

Biella, IT, Italy

RECRUITING

ASST Grande Ospedale Metropolitano Niguarda - SC Ematologia

Milan, IT, Italy

RECRUITING

Ospedale Maggiore Policlinico Fondazione IRCCS Ca' Granda - Ematologia

Milan, IT, Italy

RECRUITING

A.O.U. Maggiore della Carità di Novara - S.C.D.U. Ematologia

Novara, IT, Italy

RECRUITING

IRCCS Policlinico San Matteo - Divisione di Ematologia

Pavia, IT, Italy

RECRUITING

Ospedale Guglielmo da Saliceto - U.O. Ematologia

Piacenza, IT, Italy

RECRUITING

A.O.U. Città della Salute e della Scienza di Torino - S.C. Ematologia U

Torino, IT, Italy

RECRUITING

A.O.U. Città della Salute e della Scienza di Torino - S.C. Ematologia

Torino, IT, Italy

RECRUITING

ASST Spedali Civili - S.C. Ematologia

Brescia, Italy

RECRUITING

Azienda Ospedaliera Universitaria Careggi - Unità funzionale di Ematologia

Florence, Italy

RECRUITING

Azienda Unità Sanitaria Locale-IRCCS - Arcispedale Santa Maria Nuova - Ematologia

Reggio Emilia, Italy

RECRUITING

A.O.U. Senese - U.O.C. Ematologia

Siena, Italy

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

Peripheral blood samples

MeSH Terms

Conditions

Lymphoma, FollicularLymphomaCOVID-19TetanusHerpes ZosterDiphtheria

Condition Hierarchy (Ancestors)

Lymphoma, Non-HodgkinNeoplasms by Histologic TypeNeoplasmsLymphoproliferative DisordersLymphatic DiseasesHemic and Lymphatic DiseasesImmunoproliferative DisordersImmune System DiseasesPneumonia, ViralPneumoniaRespiratory Tract InfectionsInfectionsVirus DiseasesCoronavirus InfectionsCoronaviridae InfectionsNidovirales InfectionsRNA Virus InfectionsLung DiseasesRespiratory Tract DiseasesClostridium InfectionsGram-Positive Bacterial InfectionsBacterial InfectionsBacterial Infections and MycosesVaricella Zoster Virus InfectionHerpesviridae InfectionsDNA Virus InfectionsCorynebacterium InfectionsActinomycetales Infections

Study Officials

  • Michele Merli, MD

    U.O.C Ematologia, Ospedale di Circolo, Varese

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Uffici Studi FIL

CONTACT

Study Design

Study Type
observational
Observational Model
COHORT
Time Perspective
PROSPECTIVE
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

October 3, 2023

First Posted

October 6, 2023

Study Start

May 7, 2024

Primary Completion (Estimated)

May 1, 2027

Study Completion (Estimated)

May 1, 2027

Last Updated

June 29, 2025

Record last verified: 2025-06

Locations