A Study of Adipose Tissue in Adaptive Responses to Exercise

NCT06053125 | Active Not Recruiting

• 2 other identifiers: 23-002049K01DK134765
• Study Type: interventional
• Target: 56
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this research is to determine how exercise affects fat (adipose) tissue and how changes to adipose tissue that occur during and after exercise might improve health in aging and obesity.

Conditions

Healthy; Obesity

Keywords

exercise; adipose; cytokines

Outcome Measures

Primary Outcomes (16)

• Adipose lymphocyte populations

  The numbers of lymphocytes in the adipose tissue will be assessed by flow cytometry.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• IL6 secreted from adipose tissue measured using the Olink Target Inflammation Panel (includes 96 inflammation-associated proteins)

  The concentration of 96 IL6 will be measured using the targeted inflammation Olink proximity extension assay panel in media obtained from cultured adipose tissue explants. Adipose tissue samples will be cultured for 3 hours, and the culture media will be removed for the assessment of secreted inflammatory proteins. T

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue macrophage populations

  Numbers of macrophages in the adipose tissue will be assessed by immunohistochemistry.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue gene expression of interleukin (IL)-6 measured by polymerase chain reaction (PCR).

  Gene expression of the inflammatory cytokine IL-6 will be measured in adipose tissue samples using real time (RT)-PCR.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue gene expression of interleukin (IL)-8 measured by PCR.

  Gene expression of the inflammatory cytokine IL-8 will be assessed in adipose tissue samples using real-time (RT)-PCR.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue gene expression of cluster of differentiation 68 (CD68) measured by PCR.

  Gene expression of the macrophage marker CD68 be assessed in adipose tissue samples using real time (RT)-PCR.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue gene expression of cluster of differentiation 163 (CD163) measured by PCR.

  Gene expression of the anti-inflammatory macrophage marker CD163 will be assessed in adipose tissue samples using RT-PCR.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue gene expression of cluster of differentiation 206 (CD206) measured by PCR.

  Gene expression of the anti-inflammatory macrophage marker CD206 will be assessed in adipose tissue samples using RT-PCR.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue gene expression of adhesion G-protein coupled receptor E (ADGRE) measured by PCR.

  Gene expression of the macrophage marker ADGRE will be assessed in adipose tissue samples using RT-PCR.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue gene expression of inducible nitric oxide synthase (INOS) measured by PCR.

  Gene expression of the pro-inflammatory macrophage marker INOS will be assessed in adipose tissue samples using RT-PCR.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue gene expression of monocyte chemoattractant protein-1 (MCP1) measured by PCR.

  Gene expression of the inflammatory cytokine MCP1 will be assessed in adipose tissue samples using RT-PCR.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue gene expression of tumor necrosis factor alpha (TNFa) measured by PCR.

  Gene expression of the inflammatory cytokine TNFa will be assessed in adipose tissue samples using RT-PCR.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue gene expression of leptin (LEPD) measured by PCR.

  Gene expression of the pro-inflammatory adipokine LEPD will be assessed in adipose tissue samples using RT-PCR.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Adipose tissue gene expression of adiponectin (ADIPOQ) measured by PCR.

  Gene expression of the anti-inflammatory adipokine ADIPOQ will be assessed in adipose tissue samples using RT-PCR.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Inflammatory proteins found in adipose tissue measured using the Olink Target Inflammation Panel (includes 96 inflammation-associated proteins)

  The concentrations of 96 different inflammatory proteins (including various cytokines and adipokines) will be measured in adipose tissue lysates using the targeted inflammation Olink proximity extension assay panel. This assay measures all 96 proteins in a single assay and all are reported as normalized protein expression values.

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Protein cargo of adipose extracellular vesicles measured using mass spectrometry

  Adipose tissue samples will be cultured for 3 hours, and the culture media will be removed. From this media, extracellular vesicles will be isolated. In these extracellular vesicles, mass spectrometry will be performed to examine the entire proteome (all proteins) contained within these extracellular vesicles. Using the large number of proteins identified, the investigators will examine changes in categories of protein types (e.g., inflammatory proteins, metabolic proteins, transcriptional proteins).

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

Secondary Outcomes (3)

• Adipocyte cell size

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Plasma IL6 concentrations measured using the Olink Target Inflammation Panel

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

• Protein cargo of plasma extracellular vesicles measured using mass spectrometry extracellular vesicles

  Pre-exercise, immediately post-exercise, and 3 hours after exercise

Other Outcomes (6)

• Body fat percentage

  Baseline

• Cardiorespiratory Fitness

  Baseline

• White blood count

  Baseline

Study Arms (4)

Older adults with obesity

EXPERIMENTAL

Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.

Procedure: Adipose Tissue Biopsy; Behavioral: Exercise

Older adults without obesity

EXPERIMENTAL

Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.

Procedure: Adipose Tissue Biopsy; Behavioral: Exercise

Young adults with obesity

EXPERIMENTAL

Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.

Procedure: Adipose Tissue Biopsy; Behavioral: Exercise

Young adults without obesity

EXPERIMENTAL

Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.

Procedure: Adipose Tissue Biopsy; Behavioral: Exercise

Interventions

Adipose Tissue Biopsy PROCEDURE

Fat sample collected from abdomen before exercise, immediately after exercise, and 3 hours after exercise

Older adults with obesity; Older adults without obesity; Young adults with obesity; Young adults without obesity

Exercise BEHAVIORAL

30 minutes of exercise on a cycle ergometer exercise machine

Older adults with obesity; Older adults without obesity; Young adults with obesity; Young adults without obesity

Eligibility Criteria

• Age: 18 Years - 85 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• Adults between the ages of 18-35 OR 65-85.
• BMI 18.5 kg/m2 - 40 kg/m2
• Weight stable (≥ 3 months)
• Generally healthy

You may not qualify if:

• Participation in ≥ 30 minutes of structured physical activity ≥ 2 days per week.
• Smoking/tobacco use.
• Alcohol/substance abuse.
• Pregnancy and breastfeeding.
• Anemia.
• Abnormal renal function.
• Blood clotting disorders.
• Coronary artery disease.
• Uncontrolled thyroid disease.
• Liver disease.
• Use of medications known to influence the main outcomes of the study.
• Orthopedic problems that may be aggravated by exercise.
• Chronic disease at the discretion of the investigators.

Sponsors & Collaborators

• Mayo Clinic: lead
• National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK): collaborator

Study Sites (1)

Mayo Clinic

Rochester, Minnesota, 55905, United States

Location

Related Links

• Mayo Clinic Clinical Trials

MeSH Terms

Conditions

Obesity; Motor Activity

Interventions

Exercise

Condition Hierarchy (Ancestors)

Overweight; Overnutrition; Nutrition Disorders; Nutritional and Metabolic Diseases; Body Weight; Signs and Symptoms; Pathological Conditions, Signs and Symptoms; Behavior

Intervention Hierarchy (Ancestors)

Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Hawley Kunz, PhD

  Mayo Clinic

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NON RANDOMIZED
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Study Record Dates

• First Submitted: September 7, 2023
• First Posted: September 25, 2023
• Study Start: November 9, 2023
• Primary Completion (Estimated): October 1, 2026
• Study Completion (Estimated): October 1, 2026
• Last Updated: May 6, 2026

Record last verified: 2026-05

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)