Impact of Beetroot Supplementation in Athletes

NCT06043089 | Completed

• 1 other identifier: AWF_MSIT
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

Several previous studies observed a positive effect of beetroot supplementation on sports performance in athletes, mainly in endurance performance. However, less attention has been paid to its effects on short-duration performance, especially in youth athletes. Moreover, only a few previous investigations performed biochemical analyses, including pro-oxidant balance assessments. It is especially important because chronic nitrate intake might directly cause the production of reactive nitrogen species and reactive oxygen species in other subcellular compartments, leading to more oxidative stress. Additionally, no previous study verified the impact of beetroot intake on sleep.

Conditions

Healthy

Outcome Measures

Primary Outcomes (18)

• Performance test

  yo-yo intermittent recovery test level 1

  7 days after treatment

• Changes in nitrates

  Blood plasma will be obtained to measure nitrate concentration

  1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test

• Changes in nitrites

  Blood plasma will be obtained to measure nitrite concentration

  1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test

• Changes in prooxidant status

  Blood plasma will be obtained to measure total oxidative status

  1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test

• Changes in antioxidant status

  Blood plasma will be obtained to measure total antioxidant capacity

  1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test

• Changes in oxidative stress index

  The oxidative stress index will be calculated as the ratio of plasma prooxidant to antioxidant status

  1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test

• Changes in nitrooxidative stress markers

  Blood plasma will be obtained to measure 3-nitrotyrosine concentration

  1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test

• Changes in protein oxidation

  Blood plasma will be obtained to measure advanced protein oxidation products concentration

  1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test

• Changes in nucleic acid oxidation

  Blood plasma will be obtained to measure ox-8-hydroxy-2'-deoxyguanosine (8-OHdG) concentration

  1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test

• Changes in lipids oxidation

  Blood plasma will be obtained to measure thiobarbituric acid reactive substances concentration

  1) baseline - 2) 7th day of treatment - before the supplementation, 3) 7th day of treatment - before the performance test, 4) 7th day of treatment - immediately after the performance test

• Changes in exercise-induced metabolic acidosis

  Arterialized blood will be obtained to measure lactate concentration

  1) Before the performance test 2) 3 minutes after the performance test 3) 10 minutes after the performance test 4) 20 minutes after the performance test

• Objective Sleep - Time in bed

  Total time spent in bed during the night assessed by sleep radar

  nights 1 - 7 during treatment

• Objective Sleep - Sleep-onset latency

  The time it takes from when the athlete intends to go to sleep and actually starts to sleep assessed by sleep radar

  nights 1 - 7 during treatment

• Objective Sleep - Total sleep time

  Total sleep time obtained from sleep onset to time at wake-up assessed by sleep radar

  nights 1 - 7 during treatment

• Objective Sleep - Light sleep

  Total amount of time in light sleep assessed by sleep radar

  nights 1 - 7 during treatment

• Objective Sleep - Deep sleep

  Total amount of time in deep sleep assessed by sleep radar

  nights 1 - 7 during treatment

• Objective Sleep - REM sleep

  Total amount of time in REM sleep assessed by sleep radar

  nights 1 - 7 during treatment

• Objective Sleep - Sleep efficiency

  The percentage of total sleep time to lights off and leaving bed assessed by sleep radar

  nights 1 - 7 during treatment

Secondary Outcomes (17)

• Countermovement jump height

  7 days after treatment

• Ball speed velocity

  7 days after treatment

• Sprint test (0-20m)

  7 days after treatment

• Agility T-test

  7 days after treatment

• Maximal isometric handgrip strength

  7 days after treatment

Study Arms (2)

Placebo group

PLACEBO COMPARATOR

Athletes received 7 days of placebo (2 × 70 mL of water with beetroot powder with negligible nitrate content).

Dietary Supplement: Beetroot juice supplementation or placebo

Beetroot group

EXPERIMENTAL

Athletes received 7 days of beetroot juice (2 × 70 mL of beetroot juice ∼12.9 mmol NO3-; Beet It, James White Drinks, Ipswich, UK).

Dietary Supplement: Beetroot juice supplementation or placebo

Interventions

Beetroot juice supplementation or placebo DIETARY_SUPPLEMENT

Athletes received 7 days of beetroot juice supplementation with nitrate supplementation (2 × 70 mL of beetroot juice ∼12.9 mmol NO3-; Beet It, James White Drinks, Ipswich, UK) or placebo (2 × 70 mL of water with beetroot powder with negligible nitrate content).

Beetroot group; Placebo group

Eligibility Criteria

• Age: 14 Years - 23 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Child (0-17), Adult (18-64)

You may qualify if:

• male
• good general health assessed by physician
• highly trained
• written consent to participate

You may not qualify if:

• medication or dietary supplements used within the previous month, which could potentially impact the study outcomes
• history of sleep nor neurological disorders
• chronic use (6 months) of any antibacterial mouthwash products.

Sponsors & Collaborators

• University School of Physical Education, Krakow, Poland: lead
• University of Bergen: collaborator

Study Sites (1)

University School of Physical Education in Cracow

Krakow, 31-571, Poland

Location

Study Officials

• Aleksandra Filip-Stachnik, Phd

  University of Physcial Education in Cracow

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: PARTICIPANT, INVESTIGATOR
• Purpose: TREATMENT
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: September 5, 2023
• First Posted: September 21, 2023
• Study Start: August 23, 2023
• Primary Completion: October 16, 2023
• Study Completion: October 16, 2023
• Last Updated: February 1, 2024

Record last verified: 2024-01

Data Sharing

• IPD Sharing: Will not share

Locations

PL (1)