Time Course Change in Skeletal Muscle and Blood Phospholipid Composition With Omega-3 Fatty Acid Supplementation

NCT05971147 | Completed

• 1 other identifier: 6037330
• Study Type: interventional
• Target: 29
• Locations: 1 country
• Sites: 1

Brief Summary

Increased omega-3 fatty acid composition of human skeletal muscle phospholipids is linked to improved skeletal muscle strength and growth in women and men. However, what is unknown is if biological sex influences skeletal muscle phospholipid composition in response to omega-3 fatty acid supplementation. Moreover, whilst time course changes in skeletal muscle phospholipid composition with omega-3 fatty acid intake have been established, no study has characterized a washout of omega-3 fatty acids from skeletal muscle phospholipids following cessation of omega-3 fatty acid intake. Thus, the aim of the present investigation is to establish a time course change and washout of omega-3 fatty acids from skeletal muscle phospholipids in response to omega-3 fatty acid intake. The investigators also aim to establish if this washout is impacted by biological sex.

Conditions

Skeletal Muscle Fatty Acid Metabolism

Keywords

Omega-3 fatty acids; Eicosapentaenoic acid; Docosahexaenoic acid; Fish oil; Skeletal muscle

Outcome Measures

Primary Outcomes (1)

• Changes in skeletal muscle EPA and DHA content

  Skeletal muscle phospholipid composition will be assessed at all time points by means of gas chromatography

  At 0, 6, 8, 16, 20, and 22 weeks.

Secondary Outcomes (7)

• Changes in the expression of mitochondrial related proteins

  0, 6, 8, 16, 20, and 22 weeks.

• Changes in erythrocyte EPA and DHA content

  0, 6, 8, 16, 20, and 22 weeks.

• Changes in the expression of translational factors related to skeletal muscle protein synthesis

  0, 6, 8, 16, 20, and 22 weeks.

• Changes in circulating glucose concentrations

  0, 6, 8, 16, 20, and 22 weeks.

• Changes in circulating insulin concentrations

  0, 6, 8, 16, 20, and 22 weeks.

Study Arms (1)

Fish Oil Group

EXPERIMENTAL

All participants will be placed in this group

Dietary Supplement: Fish oil

Interventions

Fish oil DIETARY_SUPPLEMENT

Participants in this group will receive 5g fish oil with high EPA and DHA content per day

Fish Oil Group

Eligibility Criteria

• Age: 19 Years - 30 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Males and females 19-30 years
• BMI between 18-29 kg/m2
• Free of musculoskeletal injuries
• Participants not currently pregnant
• Participants willing to maintain current use of contraceptives or post-menopausal supplementation if any for the duration of the study.
• Not allergic to fish
• COVID-19 vaccinated to comply with Queen's University's return to campus guidelines, unless exempt by reasons from the Ontario Human Rights Code
• Recreationally active

You may not qualify if:

• Any muscular, neurological, respiratory or metabolic disease including diabetes
• Any form of cancer currently or in the last 5 years
• Currently taking fish oil supplements
• Currently taking any form of steroid
• Consuming \>2 oily fish meals per week
• Pregnant
• Any current illness
• Any current/past lower limb injury/surgery

Sponsors & Collaborators

• Dr. Chris McGlory, PhD: lead

Study Sites (1)

School of Kinesiology and Health Studies

Kingston, Ontario, K7L 3N6, Canada

Location

MeSH Terms

Interventions

Fish Oils

Intervention Hierarchy (Ancestors)

Oils; Lipids

Study Officials

• Chris McGlory, PhD

  Queen's University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Masking Details: All participants will take part in the same intervention, which will be fully disclosed to each participant.
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Assistant Professor

Model Details: Single-arm 5g per day of omega-3 fatty acid intake (3750 mg EPA and 1250 mg DHA)

Study Record Dates

• First Submitted: July 18, 2023
• First Posted: August 2, 2023
• Study Start: August 1, 2023
• Primary Completion: December 31, 2024
• Study Completion: January 13, 2025
• Last Updated: January 29, 2025

Record last verified: 2024-01

Data Sharing

• IPD Sharing: Will not share

Locations

CA (1)