NCT05967897

Brief Summary

The purpose of this randomized crossover trial is determine whether acute consumption of blueberries with different proteins from plants (hemp or pea) or dairy (whey) impact the absorption of blueberry phytochemicals, which could impact the health benefits of blueberries. A secondary purpose is to understand the role that the gut microbiome may play, as well as the impact on urine polyphenol metabolites and potential implications for cardiometabolic health. Researchers will compare four arms: 1) Blueberries; 2) Blueberries + whey protein; 3) Blueberries + pea protein; and 4) Blueberries + hemp protein.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
19

participants targeted

Target at below P25 for not_applicable

Timeline
Completed

Started Mar 2023

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Start

First participant enrolled

March 9, 2023

Completed
4 months until next milestone

First Submitted

Initial submission to the registry

June 22, 2023

Completed
1 month until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 26, 2023

Completed
6 days until next milestone

First Posted

Study publicly available on registry

August 1, 2023

Completed
10 months until next milestone

Study Completion

Last participant's last visit for all outcomes

June 1, 2024

Completed
Last Updated

June 6, 2024

Status Verified

June 1, 2024

Enrollment Period

5 months

First QC Date

June 22, 2023

Last Update Submit

June 5, 2024

Conditions

Keywords

HealthyAdultsBlueberriesPolyphenolsProteinBioavailability

Outcome Measures

Primary Outcomes (5)

  • Plasma polyphenol metabolites

    Plasma polyphenol metabolite analysis using mass spectrometry

    Change from 0 hours (pre-treatment consumption) to 1 hour post-treatment consumption

  • Plasma polyphenol metabolites

    Plasma polyphenol metabolite analysis using mass spectrometry

    Change from 0 hours (pre-treatment consumption) to 2 hours post-treatment consumption

  • Plasma polyphenol metabolites

    Plasma polyphenol metabolite analysis using mass spectrometry

    Change from 0 hours (pre-treatment consumption) to 4 hours post-treatment consumption

  • Plasma polyphenol metabolites

    Plasma polyphenol metabolite analysis using mass spectrometry

    Change from 0 hours (pre-treatment consumption) to 6 hours post-treatment consumption

  • Plasma polyphenol metabolites

    Plasma polyphenol metabolite analysis using mass spectrometry

    Change from 0 hours (pre-treatment consumption) to 24 hours post-treatment consumption

Secondary Outcomes (1)

  • Urine polyphenol metabolites

    0 hours (pre-treatment consumption) and 24 hours post-treatment consumption

Other Outcomes (7)

  • Gut microbial analysis

    0 hours (pre-treatment consumption) and 24 hours post-treatment consumption

  • Blood pressure

    0 hours (pre-treatment consumption) and 1, 2, 4, and 6 hours post-treatment consumption

  • Aortic pressure

    0 hours (pre-treatment consumption) and 1, 2, 4, and 6 hours post-treatment consumption

  • +4 more other outcomes

Study Arms (4)

Blueberries

EXPERIMENTAL

Freeze-dried blueberry powder

Dietary Supplement: Blueberries

Blueberries and Whey Protein

ACTIVE COMPARATOR

Freeze-dried blueberry powder and whey protein

Dietary Supplement: Blueberries and Whey Protein

Blueberries and Pea Protein

ACTIVE COMPARATOR

Freeze-dried blueberry powder and pea protein

Dietary Supplement: Blueberries and Pea Protein

Blueberries and Hemp Protein

ACTIVE COMPARATOR

Freeze-dried blueberry powder and hemp protein

Dietary Supplement: Blueberries and Hemp Protein

Interventions

BlueberriesDIETARY_SUPPLEMENT

22 g freeze-dried blueberry powder mixed with water

Blueberries

22 g freeze-dried blueberry powder mixed with whey protein and water

Blueberries and Whey Protein
Blueberries and Pea ProteinDIETARY_SUPPLEMENT

22 g freeze-dried blueberry powder mixed with pea protein and water

Blueberries and Pea Protein

22 g freeze-dried blueberry powder mixed with hemp protein and water

Blueberries and Hemp Protein

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Aged 18 years or older

You may not qualify if:

  • Blood levels of HbA1c ≥ 6.5%, total cholesterol ≥ 240 mg/dL, LDL cholesterol ≥ 130 mg/dL, triglycerides ≥ 200 mg/dL
  • Are anemic (hemoglobin less than 13.5 g/dL in men and 12.0 g/dL in women)
  • Have an average blood pressure greater than or equal to 130/80 mmHg, or have diagnosed hypertension, hyperlipidemia, cardiovascular disease, diabetes, cancer, kidney, liver, gastrointestinal or pancreatic disease
  • Taking blood pressure-, lipid- or glucose-lowering, or hormone replacement medications
  • Are pregnant or are trying to become pregnant
  • Have a body mass index \< 18.5 or \> 30 kg/m2
  • \>3 days/week vigorous exercise
  • Are not willing to maintain your normal eating/drinking and exercise habits over the duration of the study
  • History of smoking cigarettes in past 12 months
  • Binge and/or heavy drinker (\>3 drinks on any given occasion and/or \>7 drinks/week for women, and \>4 drinks on any given occasion and/or \>14 drinks/week for men)
  • Have allergies or contraindication to study treatments, procedures, or procedure supplies
  • Unable to attend study visits.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Food and Nutrition Clinical Research Laboratory - CSU Gifford Building

Fort Collins, Colorado, 80523-1571, United States

Location

Related Publications (1)

  • Chima B, Mathews P, Morgan S, Johnson SA, Van Buiten CB. Physicochemical Characterization of Interactions between Blueberry Polyphenols and Food Proteins from Dairy and Plant Sources. Foods. 2022 Sep 14;11(18):2846. doi: 10.3390/foods11182846.

    PMID: 36140972BACKGROUND

MeSH Terms

Interventions

blueberry extractWhey ProteinsPea Proteins

Intervention Hierarchy (Ancestors)

Milk ProteinsAnimal Proteins, DietaryDietary ProteinsProteinsAmino Acids, Peptides, and ProteinsWheyMilkDairy ProductsFoodDiet, Food, and NutritionPhysiological PhenomenaFood and BeveragesPlant Proteins, DietaryPlant Proteins

Study Officials

  • Sarah A Johnson, PhD, RDN

    Colorado State University, Department of Food Science and Human Nutrition

    PRINCIPAL INVESTIGATOR
  • Charlene Van Buiten, PhD

    Colorado State University, Department of Food Science and Human Nutrition

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
OUTCOMES ASSESSOR
Purpose
BASIC SCIENCE
Intervention Model
CROSSOVER
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 22, 2023

First Posted

August 1, 2023

Study Start

March 9, 2023

Primary Completion

July 26, 2023

Study Completion

June 1, 2024

Last Updated

June 6, 2024

Record last verified: 2024-06

Locations