Resistance Exercise and Hydrolyzed Collagen Supplementation

NCT05932771 | Completed

• 1 other identifier: 18/SPS/059
• Study Type: interventional
• Target: 21
• Locations: 1 country
• Sites: 1

Brief Summary

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.

Conditions

Diet, Food, and Nutrition

Keywords

Hydolyzed collagen; Resistance exercise; Collagen synthesis; Proline; Glycine

Outcome Measures

Primary Outcomes (3)

• Change in a marker of collagen synthesis

  Serum procollagen type Ⅰ N-terminal propeptide (PⅠNP) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.

  at rest immediately prior to HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE

• Change in a marker of collagen breakdown

  Plasma β-isomerized C-terminal telopeptide of type I collagen (β-CTX) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.

  at rest immediately prior to HC ingestion and 6-hour post RE

• Changes in amino acids concentrations in blood

  Concentration of serum amino acids that constitute collagen was measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.

  at rest immediately prior to HC ingestion, 0.5-hour post HC ingestion, 1-hour post HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE

Secondary Outcomes (1)

• Estrogen concentrations in women in each trial

  at rest immediately prior to HC ingestion

Study Arms (4)

1.Trial for young male participants: Consuming hydrolyzed collagen (HC) with reistance exercise

EXPERIMENTAL

Young male participants consumed one of three different HC doses (0 grams, 15 grams, or 30 grams) with 4 sets of 10 repetitions of barbell back squat exercise at 10-repetition maximum load in a random order and a seven-day wash-out period interspersed between each trial.

Dietary Supplement: Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise

2.Trial for young female participant: Consuming hydrolyzed collagen (HC) with reistance exercise

EXPERIMENTAL

The intervention procedure is exactly same as Arm 1 except for the number of visits. Young female participant was asked to visit the laboratory on four occasions during two consecutive months. Therefore, there were two trials in each month, where female participants' estrogen level was lower (i.e., onset of menses) or higher (i.e., ovulation). Dates for the trials were determined based on self-report of onset of menses and previous menstrual cycle length.

Dietary Supplement: Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise

3.Trial for older male participants: Consuming hydrolyzed collagen (HC) with reistance exercise

EXPERIMENTAL

The age range of older male participants was 40 - 65 years. The intervention procedure is exactly same as Arm 1

Dietary Supplement: Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise

4.Trial for older female participants: Consuming hydrolyzed collagen (HC) with reistance exercise

EXPERIMENTAL

The age range of older female participants was 40 - 65 years. The intervention procedure is exactly same as Arm 2

Dietary Supplement: Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise

Interventions

Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise DIETARY_SUPPLEMENT

Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula. All trials were performed at the same time of day (08:00 - 15:00). Different doses of HC (0 grams, 15 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams and 15.4 grams maltodextrin was used in 0 grams HC and 15 grams HC respectively. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses. The details of nutritoinal supplements used are as follows: Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)

Also known as: Resistance exercise and collagen dose-response for male participants

1.Trial for young male participants: Consuming hydrolyzed collagen (HC) with reistance exercise; 3.Trial for older male participants: Consuming hydrolyzed collagen (HC) with reistance exercise

Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise DIETARY_SUPPLEMENT

Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula. All trials were performed at the same time of day (08:00 - 15:00). Different doses of HC (0 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams maltodextrin was used in 0 grams HC. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses. The details of nutritoinal supplements used are as follows: Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)

Also known as: Resistance exercise and collagen dose-response for female participants

2.Trial for young female participant: Consuming hydrolyzed collagen (HC) with reistance exercise; 4.Trial for older female participants: Consuming hydrolyzed collagen (HC) with reistance exercise

Eligibility Criteria

• Age: 18 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)

You may qualify if:

• At least 12 months experienced in resistance exercise (regular back squat experience)
• Free from musculoskeletal injury
• Nullipara (a woman who has never given birth) (only for young female participatns)

You may not qualify if:

• Having a history of patellar tendon pathology
• Vegan
• Consumed nutritional supplements or medication purported to have beneficial effects on muscle-tendon properties (e.g. antioxidants, protein, etc.)
• Had a sustained a lower limb injury in the previous six months
• Smoker/vaper
• \<18 or \>40 years old (only for young participants)
• \<40 ro \>65 years old (only for older participants)
• Having an irregular menstrual cycle (only for young female participants)

Sponsors & Collaborators

• Rob Erskine: lead
• University of East Anglia: collaborator

Study Sites (1)

Dr Rob Erskine

Liverpool, L3 3AF, United Kingdom

Location

Study Officials

• Robert M. Erskine, PhD

  Liverpool John Moores University

  STUDY DIRECTOR

• Joonsung Lee, MSc

  Liverpool John Moores University

  PRINCIPAL INVESTIGATOR

• Christopher Nulty

  Liverpool John Moores University

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Masking Details: Before commencing each experimental trial, a laboratory technician (independent to the study) made up the three doses of hydrolyzed collagen (HC) and randomly assigned the order of HC dose (Excel 2016, Microsoft, Washington, USA) for each participant. Also, for each trial, the technician recorded the date, randomly allocated trial number (1, 2 or 3) and corresponding HC dose to blind the investigator.
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: SPONSOR INVESTIGATOR
• PI Title: Reader in Neuromuscular Physiology

Model Details: A double-blind, randomised cross-over design

Study Record Dates

• First Submitted: May 26, 2023
• First Posted: July 6, 2023
• Study Start: January 1, 2019
• Primary Completion: August 30, 2019
• Study Completion: April 30, 2023
• Last Updated: July 6, 2023

Record last verified: 2023-06

Data Sharing

• IPD Sharing: Will not share

Locations

GB (1)