Isolation and Characterization of Blastocystis Hominis as Apotential Human Pathogen

NCT05814861 | Unknown

• 1 other identifier: Blastocystis hominis isolation
• Study Type: observational
• Target: 72
• Locations: 0 countries
• Sites: N/A

Brief Summary

• Detection of the presence of Blastocystis hominis among outpatients presented with GIT symptoms at Assiut University Hospitals in comparison with those in control group.
• Assessment of different culture techniques for detection, and isolation of Blastocystis hominis.
• Molecular identification of Blastocystis hominis using polymerase chain reaction (PCR).

Conditions

Blastocystis Hominis Infections

Outcome Measures

Primary Outcomes (1)

• -Morphological identification of Blastocystis hominis in stool.

  Microscopic Detection of Blastocystis hominis among outpatients presented with GIT symptoms at Assiut University Hospitals .

  1year

Secondary Outcomes (1)

• Molecular identification of Blastocystis hominis

  1 year

Study Arms (2)

patients presented with GIT symptoms

Fresh Stool samples will be collected in clean sterile cups from patients suffering from gastrointestinal symptoms attending Assiut University Hospitals and examined for detection of Blastocystis hominis

Diagnostic Test: culture and PCR

clinically free persons undergo routine stool analysis for any reasons other than GIT complaint

Fresh Stool samples will be collected in clean sterile cups from clinically free persons under go routine stool examination for any cause other than GIT troubles and examined for detection of Blastocystis hominis

Diagnostic Test: culture and PCR

Interventions

culture and PCR DIAGNOSTIC_TEST

Stool samples will be cultured inTwo media : A-Locke egg serum medium (LE) B- Jones' medium Then Molecular identification of Blastocystis hominis using polymerase chain reaction (PCR).

clinically free persons undergo routine stool analysis for any reasons other than GIT complaint; patients presented with GIT symptoms

Eligibility Criteria

• Sex: all
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

The persons selected for the study are those who are: 1. Suffering from any GIT manifestations, attending outpatient clinics of Internal Medicine and Tropical Medicine Departments at Assiut University Hospitals (case group) * GIT manifestations such as diarrhea; (frequency ≥3 loose stool /day), either acute diarrhea (duration less than 2 weeks), or chronic diarrhea (duration more than 4 weeks), or other GIT complaints as abdominal pain, flatulence, nausea, vomiting, or weight loss. 2. clinically free persons undergo routine stool analysis for any reasons other than GIT complaint.(control group)

You may qualify if:

• The persons selected for the study are those who are:
• Suffering from any GIT manifestations, attending outpatient clinics of Internal Medicine and Tropical Medicine Departments at Assiut University Hospitals (case group)
• clinically free persons undergo routine stool analysis for any reasons other than GIT complaint.(control group)

You may not qualify if:

• We will exclude any patient who received antibiotic or anti-parasitic drugs the past 4 weeks prior to sample collection.

Sponsors & Collaborators

• Assiut University: lead

Related Publications (3)

• A. F. Bafghi, R. Hosseini, H. R. Mollaei, and K. Barzegar, "Geno-typing and Comparison of Conventional and Molecular Diagnostic Techniques for Detection of Blastocystis on health centers in Kerman , Iran," vol. 8, no. 01, pp. 10-16, 2021..

  BACKGROUND

• S M. Journal, "SOHAG MEDICAL JOURNAL Vol. 21 No.3," vol. 21, no. 3, 2017.

  BACKGROUND

• M. Q. Khairuzzaman, "No Title血清及尿液特定蛋白检测在糖尿病肾病早期诊断中的意义," vol. 4, no. 1, pp. 64-75, 2016.

  BACKGROUND

MeSH Terms

Conditions

Blastocystis Infections

Condition Hierarchy (Ancestors)

Intestinal Diseases, Parasitic; Parasitic Diseases; Infections; Amebiasis; Protozoan Infections; Intestinal Diseases; Gastrointestinal Diseases; Digestive System Diseases

Central Study Contacts

Fatma Alzahraa montaser Ali, demonstrator

CONTACT

01060093655; docfatma490@gmail.com

magda mohamed atiya Elnazer, Prof.

CONTACT

01143533100; Magda.elnazer@med.aun.edu.eg

Study Design

• Study Type: observational
• Observational Model: CASE CONTROL
• Time Perspective: CROSS SECTIONAL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Investigator

Study Record Dates

• First Submitted: March 20, 2023
• First Posted: April 18, 2023
• Study Start: December 1, 2023
• Primary Completion: October 1, 2024
• Study Completion: December 31, 2024
• Last Updated: April 18, 2023

Record last verified: 2023-03

Data Sharing

• IPD Sharing: Will not share