Trichomonas Vaginalis Genotyping in Upper Egypt

NCT03614286 | Unknown

• 1 other identifier: T V Genotyping
• Study Type: observational
• Target: 20
• Locations: 0 countries
• Sites: N/A

Brief Summary

The worldwide incidence of trichomoniasis was estimated to be 276.4 million new cases per year in 2008 .In Egypt, the reported prevalence rate ranges from 5% to 79.16% we aim to study genetic variability of Trichomonas vaginalis using PCR

Conditions

Trichomonas Vaginalis Genotyping in Upper Egypt; Vaginitis Trichomonal

Outcome Measures

Primary Outcomes (1)

• study genetic variability of Trichomonas vaginalis using PCR

  detection of TV in vaginal swap then PCR done to detect genetic diversity

  1year

Interventions

culture and pcr DIAGNOSTIC_TEST

The swab immersed in Diamond's Modified medium culture tube and squeezed for cultivation and examined daily with a light microscope to identify T. vaginalis Samples from culture will be placed in 1 ml of a commercial PCR transport medium (AMPLICOR; Roche Diagnostic Systems, Branchburg, N.J.) and kept at 4°C until arrival at the laboratory within 4 days of collection. An equal volume of specimen diluent (AMPLICOR) was added to the sample , and the preparation was mixed, incubated at room temperature for 10 min, and stored at -70°C until tested.

Also known as: PCR

Eligibility Criteria

• Gender Eligibility Details: non virgins females
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

one group from 20 patients

You may qualify if:

• females ,non virgins,complain from vaginitis ,at reproductive age and attending to sohag general hospital outpatient clinic of gynecology and obstetric

Sponsors & Collaborators

• Assiut University: lead

Related Publications (1)

• Zhang Z, Kang L, Wang W, Zhao X, Li Y, Xie Q, Wang S, He T, Li H, Xiao T, Chen Y, Zuo S, Kong L, Li P, Li X. Prevalence and genetic diversity of Trichomonas vaginalis clinical isolates in a targeted population in Xinxiang City, Henan Province, China. Parasit Vectors. 2018 Mar 2;11(1):124. doi: 10.1186/s13071-018-2753-4.

  PMID: 29499747 BACKGROUND

Biospecimen

Retention: SAMPLES WITH DNA

vaginal swab

MeSH Terms

Conditions

Trichomonas Vaginitis

Condition Hierarchy (Ancestors)

Trichomonas Infections; Protozoan Infections; Parasitic Diseases; Infections; Vaginitis; Vaginal Diseases; Genital Diseases, Female; Female Urogenital Diseases; Female Urogenital Diseases and Pregnancy Complications; Urogenital Diseases; Genital Diseases

Central Study Contacts

Ahmed Kamal Dyab, Professor

CONTACT

+201018614645; ahmedsaf2001@yahoo.com

Mohamed Essa, Professor

CONTACT

+201006329838; essa3eg@yahoo.com

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: OTHER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Lecturer of Medical Parasitology

Study Record Dates

• First Submitted: July 30, 2018
• First Posted: August 3, 2018
• Study Start: October 1, 2018
• Primary Completion: October 1, 2019
• Study Completion: March 1, 2020
• Last Updated: August 7, 2018

Record last verified: 2018-08