Evaluation of the Relationship Between Bruxism and Tryptophan Metabolites

NCT05760482 | Completed

• 1 other identifier: SelcukU002
• Study Type: interventional
• Target: 92
• Locations: 1 country
• Sites: 1

Brief Summary

Psychological causes are often cited as the most important of the underlying factors for bruxism. However, there are very few studies that can objectively demonstrate this. There are studies that are generally based on subjective data, that is, on questionnaires that indicate anxiety or stress. Recent studies have shown that tryptophan and its metabolites are associated with psychological health. In this study, researchers will measure the serum values of metabolites in the tryptophan pathway. Also, researchers will observe whether these metabolite levels differ significantly in patients with and without bruxism.

Conditions

Bruxism; Anxiety State; Tryptophan Metabolism Alterations

Outcome Measures

Primary Outcomes (6)

• serum tryptophane level measurement

  serum tryptophane level measurement

  blood sample will be taken 5 minutes after the patient sits the dental unit

• serum kynurenin level measurement

  serum kynurenin level measurement

  blood sample will be taken 5 minutes after the patient sits the dental unit

• serum kynurenic acid level measurement

  serum kynurenic acid level measurement

  blood sample will be taken 5 minutes after the patient sits the dental unit

• serum quinolinic acid level measurement

  serum quinolinic acid level measurement

  blood sample will be taken 5 minutes after the patient sits the dental unit

• serum 3-hydroxykynurenin level measurement

  serum 3-hydroxykynurenin(3-HK) level measurement

  blood sample will be taken 5 minutes after the patient sits the dental unit

• serum 3-hydroxyanthranilic level measurement

  serum 3-hydroxyanthranilic(3-HAA) level measurement

  blood sample will be taken 5 minutes after the patient sits the dental unit

Study Arms (2)

Bruxism Group

EXPERIMENTAL

Diagnostic Test: Metobolites serum level measurement; Behavioral: Anxiety and stress level measurement

Control Group

EXPERIMENTAL

Diagnostic Test: Metobolites serum level measurement; Behavioral: Anxiety and stress level measurement

Interventions

Metobolites serum level measurement DIAGNOSTIC_TEST

Blood samples will be taken from all volunteer participants in 1 yellow cap tube of 10 cc. After the blood has cooled and the clot development process (approximately 15 minutes), the serum will be separated by centrifugation at 2000 g for 5 minutes. Separated sera will be aliquoted and stored at -40°C until analysis. The tryptophan and tryptophane metabolite levels of the participants will be measured by Liquid Chromatography-Tandem Mass Spectrometer API 3200 device. The kynurenine, kynurenic acid, 3-HK, 3-HAA, 3-HOA, and QA values obtained as a result of this test will be recorded.

Bruxism Group; Control Group

Anxiety and stress level measurement BEHAVIORAL

Anxiety and stress levels will be evaluated by applying State-Trait Anxiety Inventory and Perceived Stress Scale Tests by all volunteer participants. Scores from these tests will be recorded.

Bruxism Group; Control Group

Eligibility Criteria

• Age: 18 Years - 50 Years
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64)

You may qualify if:

• age range 18-50
• applying to our clinic with the complaint of bruxism
• no psychological drug
• no active orthodontic treatment
• no musculoskeletal disease
• no removable prosthesis
• no obstructive sleep apnea syndrome
• "Class I" according to RDC/TMD classification
• "probable" according to bruxism classifiacation
• accept to fill out the questionnaire
• accept to give a blood sample
• being volunteer
• accept to sign the informed consent

You may not qualify if:

• indicate to quit the study at anytime
• not fully fill out the questionnaire
• encountering any complication during taking a blood sample
• can not take an adequate blood sample
• presenting any below diseases;
• acute myocardial infarction
• pulmonary emboli
• deep venous thrombosis
• heart failure
• sepsis
• cerebral edema
• hypertensive encephalopathy
• hypoglycemia
• hepatic encephalopathy
• fluid-electrolyte disturbances

Sponsors & Collaborators

• Selcuk University: lead

Study Sites (1)

Selcuk University, Faculty of Dentistry

Konya, Selçuklu, 42000, Turkey (Türkiye)

Location

MeSH Terms

Conditions

Bruxism; Anxiety Disorders

Condition Hierarchy (Ancestors)

Tooth Diseases; Stomatognathic Diseases; Habits; Behavior; Mental Disorders

Study Officials

• Gökhan Gürses, Assistant Professor

  Selcuk University, Faculty of Dentistry

  STUDY CHAIR

• Ali Ünlü, Professor

  Selcuk University, Faculty of Medicine

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: SINGLE
• Who Masked: OUTCOMES ASSESSOR
• Purpose: DIAGNOSTIC
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Assistant Professor

Study Record Dates

• First Submitted: February 26, 2023
• First Posted: March 8, 2023
• Study Start: September 4, 2023
• Primary Completion: May 31, 2024
• Study Completion: June 1, 2024
• Last Updated: June 6, 2024

Record last verified: 2024-06

Locations

TU (1)