NCT05755672

Brief Summary

By virtue of an increased strategic use of cytotoxic and biological agents, and more options for locoregional treatment, the survival of patients with metastatic colorectal cancer (mCRC) has improved considerably in the past decades. The personalized approach to systemic treatment is further aided by the use of complementary molecular biomarkers. However, the evolutionary dynamics of mCRC, a disease harnessed by multiple adaptive genetic alterations towards its final stages, poses a particular challenge to single-sample biomarker analyses and standardized linear treatment protocols. The aim of the On-treatment biomarkers in metastatic ColorectAL cancer for Life (On-CALL) study is to generate further knowledge on the evolutionary progression of mCRC during treatment, and to elucidate the mechanisms underlying the therapeutic failure still seen in a substantial number of patients. The On-CALL study is a prospective, single-arm observational study. All patients diagnosed with synchronous mCRC treated with curative intent at Skåne University Hospital will be invited to participate. Clinical and histopathological data will be compiled at study entry. An individual tissue microarray block with samples from resected primary tumours and metastases representing the full extent of the tumour spread will be constructed for each patient. Blood samples will be drawn for biomarker analyses at multiple time points prior to, during and after systemic treatment. DNA sequencing of tumour tissue and circulating tumour DNA (ctDNA) will be performed to define the spatial clonal landscape in primary tumours and metastases, as well as over time.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
100

participants targeted

Target at P50-P75 for all trials

Timeline
83mo left

Started Mar 2023

Longer than P75 for all trials

Geographic Reach
1 country

2 active sites

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress32%
Mar 2023Mar 2033

First Submitted

Initial submission to the registry

February 17, 2023

Completed
12 days until next milestone

Study Start

First participant enrolled

March 1, 2023

Completed
5 days until next milestone

First Posted

Study publicly available on registry

March 6, 2023

Completed
3.7 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 1, 2026

Expected
6.3 years until next milestone

Study Completion

Last participant's last visit for all outcomes

March 1, 2033

Last Updated

July 17, 2025

Status Verified

July 1, 2025

Enrollment Period

3.8 years

First QC Date

February 17, 2023

Last Update Submit

July 14, 2025

Conditions

Keywords

Metastatic colorectal cancerChemotherapyTargeted therapyTumor heterogeneityTumor evolution

Outcome Measures

Primary Outcomes (1)

  • Follow-up examination of tumour remission, progression or recurrence

    Radiological/clinical examination of tumour remission, progression or recurrence, and correlation of this clinical information with the available oncogenetic data from histological samples from the primary tumour and metastases, and from data from blood samples (ctDNA analysis) * Oncogenetic data will come from genomic profiling of tissue samples. This will be carried out by targeted deep sequencing (TDS), using a comprehensive panel covering cancer-related genes, in combination with genome wide SNP array for detection of copy number aberrations. * Data from blood samples will be extracted through quantification of ctDNA and monitoring of the temporal clonal dynamics (circulating cell free DNA will be extracted from plasma samples from all time points).

    10 years

Secondary Outcomes (1)

  • Quality of life changes

    10 years

Study Arms (1)

Synchronous mCRC patients

Patients diagnosed with synchronous metastatic colorectal cancer with planned treatment with curative intent at Skåne University Hospital (Malmö and Lund), who have accepted the study invitation (agreeing to participation - informed consent)

Drug: ChemotherapyProcedure: Resection of the primary tumorProcedure: Resection of tumour metastasesProcedure: Blood sampling during chemotherapy

Interventions

Neo-adjuvant and adjuvant chemotherapy with curative intent, according to the current clinical practice guidelines

Synchronous mCRC patients

Surgical resection of the primary tumour, according to the current clinical practice guidelines

Also known as: Surgical resection of the primary tumour
Synchronous mCRC patients

Surgical resection of tumour metastases, according to the current clinical practice guidelines

Also known as: Surgical resection of tumour metastases
Synchronous mCRC patients

Drawing of blood samples several times during patient visits for chemotherapy regimen

Also known as: Drawing of blood samples during chemotherapy
Synchronous mCRC patients

Eligibility Criteria

Age18 Years - 110 Years
Sexall
Healthy VolunteersNo
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodNon-Probability Sample
Study Population

All patients who are treated at the Skåne University Hospital

You may qualify if:

  • Clinical diagnosis of synchronous metastatic colorectal cancer, planned cancer treatment with curative intent at the Skåne University Hospital

You may not qualify if:

  • Age below or above the age limit

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Department of Oncology, Hematology and Radiophysics, Skane University Hospital

Lund, Skåne County, 22241, Sweden

RECRUITING

Department of Oncology, Hematology and Radiophysics, Skane University Hospital

Malmo, Skåne County, 21428, Sweden

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

* Tissue from the first histological specimen for diagnosis (usually a biopsy) * Blood sample during ongoing neoadjuvant and/or adjuvant chemotherapy * Tissue from the surgical resection specimen (primary tumour and resected metastases)

MeSH Terms

Conditions

Colorectal Neoplasms

Interventions

Drug Therapy

Condition Hierarchy (Ancestors)

Intestinal NeoplasmsGastrointestinal NeoplasmsDigestive System NeoplasmsNeoplasms by SiteNeoplasmsDigestive System DiseasesGastrointestinal DiseasesColonic DiseasesIntestinal DiseasesRectal Diseases

Intervention Hierarchy (Ancestors)

Therapeutics

Study Officials

  • Karin Jirström, M.D. PhD.

    Region Skåne

    STUDY DIRECTOR
  • Jakob Eberhard, M.D. PhD.

    Region Skåne

    STUDY CHAIR

Central Study Contacts

Christina Siesing, M.D. PhD.

CONTACT

Study Design

Study Type
observational
Observational Model
CASE ONLY
Time Perspective
PROSPECTIVE
Target Duration
10 Years
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

February 17, 2023

First Posted

March 6, 2023

Study Start

March 1, 2023

Primary Completion (Estimated)

December 1, 2026

Study Completion (Estimated)

March 1, 2033

Last Updated

July 17, 2025

Record last verified: 2025-07

Locations