Effects of Soluble Dietary Fiber on Sport Efficiency and Fatigue Delay in Top Basketball Players

NCT05726435 | Completed

• 1 other identifier: IP-2016-06-3509
• Study Type: interventional
• Target: 20
• Locations: 1 country
• Sites: 1

Brief Summary

Athlete nutrition is becoming an increasingly important factor on the path to achieving top results. Nutrients such as soluble fiber can alter the intestinal microbiota, resulting in metabolites with a positive effect on metabolism. Thus, the fibers become available, an additional source of energy to the host, have an anti-stress effect and delay the feeling of fatigue. The primary goal of the study is to determine the impact of soluble dietary fiber on increasing athletic efficiency, the secondary goal is to determine the impact of fiber on delaying fatigue. Subjects will be randomly divided into experimental and control group. The sample of variables will consist of genome microbiota genome sequencing data, measures to assess morphological characteristics, lower extremity explosive strength tests, and cardiovascular endurance. The statistical package SPSS will be used for data processing. The expected scientific contribution of this research is to expand the scientific knowledge about the importance of dietary fiber in the diet of athletes with the aim of improving sports performance.

Conditions

Dietary Fiber; Sports; Sports Nutrition; Microbiota; Nutrition Surveys

Keywords

Fiber; Gastrointestinal Microbiota; Nutrition; 16S rRNA sequencing; Performance

Outcome Measures

Primary Outcomes (3)

• Rapid microbiota change

  The impact of fiber supplementation on gut microbiome shall assessed through monitoring of relative taxonomic abundance of bacteria that compose gut microbiota. DNA shall be extracted from collected faecal samples, stored at -80°C. DNA extraction shall be performed using the QIAamp® PowerFecal® DNA Kit. DNA concentration shall be quantified using Promega Quantus Flourometer before being sent to amplicon sequencing of variable regions 3 and 4 of the 16S rRNA gene with the primer set 341F (5'-CCTAYGGGRBGCASCAG -3') 806R (5'- GGACTACNNGGGTATCTAAT -3'). Obtained sequenceing raw data (reads) shall be analyzed using 'Quantitative Insights Into Microbial Ecology 2' (QIIME2) software in order to evaluate microbial composition of each sample. The comparison on intial sample (before intervention) and sample 1 (after 2 weeks) of supplementation shall provide a baseline insight into eventual rapid microbiota change as a response to supplementation/placebo.

  2 weeks

• Altered microbiota composition

  Prolonged supplementation after initial 2 weeks will support the established altered gut microbiota in participants receiving fiber supplement. The impact of fiber supplementation on gut microbiome shall assessed through monitoring of relative taxonomic abundance of bacteria that compose gut microbiota. Gut microbiota DNA shall be extracted from collected faecal samples, stored at -80°C. DNA shall be processed in the same manner as previously described (Outcome 1). Obtained sequenceing raw data (reads) shall be analyzed using 'Quantitative Insights Into Microbial Ecology 2' (QIIME2) software in order to evaluate microbial composition of each sample. The comparison on intial sample (before intervention) and sample 2 (after 4 weeks) of supplementation shall provide an insight into eventual microbiota change as a response to prolonged supplementation/placebo.

  4 weeks

• Effect on the perception of fatigue

  The major question is whether the moderately increasing fiber intake in athletes has the potential to support the intestinal microbiota and conversely positively impact training-induced perception of fatigue. In order to understand the impact of fiber consumption on the perception of fatigue, after each training session or match, the participants shall be asked to record and send a rating of perceived exertion through a mobile messaging application. The Borg's CR10 RPE scale shall be used for this purpose. This scale has a range from 0 to 10 (RPE - Rating of Perceived Exertion). The RPE scale is a tool used to monitor the perceptual response to training, as a method of determining physical exertion during exercise. The original RPE scale, developed more than 40 years ago, was primarily used to monitor aerobic load, but it still remains widely used in athlete tracking.

  4 weeks

Secondary Outcomes (1)

• Long-term microbiota impact

  8 weeks

Study Arms (2)

Experimental group

ACTIVE COMPARATOR

Seven randomly selected active basketball players will be supplemented with defined prebiotic fibre (20 grams per day divided in two 10 gram doses - 85% total fiber content) for the total duration of 4 weeks, while being stationed in the training camp under constant coach and nutrition expert surveillance.

Dietary Supplement: Soluble dietary fiber with prebiotic properties

Control group

PLACEBO COMPARATOR

Seven randomly selected active basketball players will be supplemented with placebo resembling prebiotic fibre in all visible and taste aspects (20 grams per day divided in two 10 gram doses) for the total duration of 4 weeks, while being stationed in the training camp under constant coach and nutrition expert surveillance.

Dietary Supplement: Placebo

Interventions

Soluble dietary fiber with prebiotic properties DIETARY_SUPPLEMENT

14 adult professional baskeyball players will be divided randomly in two groups, each containing 7 players. One group shall receive Nutriose fiber, two times per day 10 grams per dose in total duration of 4 weeks. Second group shall receive same amount of Placebo in form of Maltodextrin. Each group will undergo a series of sports performance tests and questionnaires and shall donate their feces several times during the study in order to monitor gut microbiota changes. The Nutriose group in this particular intervention contains 7 randomy selected players.

Also known as: Nutriose

Experimental group

Placebo DIETARY_SUPPLEMENT

14 adult professional baskeyball players will be divided randomly in two groups, each containing 7 players. One group shall receive Nutriose fiber, two times per day 10 grams per dose in total duration of 4 weeks. Second group shall receive same amount of Placebo in form of Maltodextrin. Each group will undergo a series of sports performance tests and questionnaires and shall donate their feces several times during the study in order to monitor gut microbiota changes. This second (Placebo) group also consists of the 7 players and receives Maltodextrin supplementation. Since the study type is "double-blind", neither the participants (players) nor the person handing out the supplementation know the exact nature of fiber substance being handed during the entire duration of the trial. Independent statistician is handling the randomization of both interventions.

Also known as: Maltodextrin

Control group

Eligibility Criteria

• Age: 18 Years - 22 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Professional basketball players from the same team participating in the same monitored training process

You may not qualify if:

• Diabetes or other chronic disease, taking sports performance enhancing drugs, taking antibiotics 3 months prior or during the course of the study

Sponsors & Collaborators

• University of Zagreb: lead
• Croatian Science Foundation: collaborator

Study Sites (1)

Faculty of Food Technology and Biotechnology

Zagreb, 10000, Croatia

Location

Related Publications (11)

• Wosinska L, Cotter PD, O'Sullivan O, Guinane C. The Potential Impact of Probiotics on the Gut Microbiome of Athletes. Nutrients. 2019 Sep 21;11(10):2270. doi: 10.3390/nu11102270.

  PMID: 31546638 BACKGROUND

• Marttinen M, Ala-Jaakkola R, Laitila A, Lehtinen MJ. Gut Microbiota, Probiotics and Physical Performance in Athletes and Physically Active Individuals. Nutrients. 2020 Sep 25;12(10):2936. doi: 10.3390/nu12102936.

  PMID: 32992765 BACKGROUND

• Clark A, Mach N. Exercise-induced stress behavior, gut-microbiota-brain axis and diet: a systematic review for athletes. J Int Soc Sports Nutr. 2016 Nov 24;13:43. doi: 10.1186/s12970-016-0155-6. eCollection 2016.

  PMID: 27924137 BACKGROUND

• Mohr AE, Jager R, Carpenter KC, Kerksick CM, Purpura M, Townsend JR, West NP, Black K, Gleeson M, Pyne DB, Wells SD, Arent SM, Kreider RB, Campbell BI, Bannock L, Scheiman J, Wissent CJ, Pane M, Kalman DS, Pugh JN, Ortega-Santos CP, Ter Haar JA, Arciero PJ, Antonio J. The athletic gut microbiota. J Int Soc Sports Nutr. 2020 May 12;17(1):24. doi: 10.1186/s12970-020-00353-w.

  PMID: 32398103 BACKGROUND

• Erdogan Z, Erdogan S, Aslantas O, Celik S. Effects of dietary supplementation of synbiotics and phytobiotics on performance, caecal coliform population and some oxidant/antioxidant parameters of broilers. J Anim Physiol Anim Nutr (Berl). 2010 Oct;94(5):e40-8. doi: 10.1111/j.1439-0396.2009.00973.x.

  PMID: 20487099 BACKGROUND

• Lefranc-Millot C, Guerin-Deremaux L, Wils D, Neut C, Miller LE, Saniez-Degrave MH. Impact of a resistant dextrin on intestinal ecology: how altering the digestive ecosystem with NUTRIOSE(R), a soluble fibre with prebiotic properties, may be beneficial for health. J Int Med Res. 2012;40(1):211-24. doi: 10.1177/147323001204000122.

  PMID: 22429361 BACKGROUND

• Farhangi MA, Javid AZ, Sarmadi B, Karimi P, Dehghan P. A randomized controlled trial on the efficacy of resistant dextrin, as functional food, in women with type 2 diabetes: Targeting the hypothalamic-pituitary-adrenal axis and immune system. Clin Nutr. 2018 Aug;37(4):1216-1223. doi: 10.1016/j.clnu.2017.06.005. Epub 2017 Jun 10.

  PMID: 28669666 BACKGROUND

• Aliasgharzadeh A, Dehghan P, Gargari BP, Asghari-Jafarabadi M. Resistant dextrin, as a prebiotic, improves insulin resistance and inflammation in women with type 2 diabetes: a randomised controlled clinical trial. Br J Nutr. 2015 Jan 28;113(2):321-30. doi: 10.1017/S0007114514003675.

  PMID: 27028002 BACKGROUND

• Li S, Guerin-Deremaux L, Pochat M, Wils D, Reifer C, Miller LE. NUTRIOSE dietary fiber supplementation improves insulin resistance and determinants of metabolic syndrome in overweight men: a double-blind, randomized, placebo-controlled study. Appl Physiol Nutr Metab. 2010 Dec;35(6):773-82. doi: 10.1139/H10-074.

  PMID: 21164548 BACKGROUND

• Guerin-Deremaux L, Pochat M, Reifer C, Wils D, Cho S, Miller LE. The soluble fiber NUTRIOSE induces a dose-dependent beneficial impact on satiety over time in humans. Nutr Res. 2011 Sep;31(9):665-72. doi: 10.1016/j.nutres.2011.09.004.

  PMID: 22024490 BACKGROUND

• Nazare JA, Sauvinet V, Normand S, Guerin-Deremaux L, Gabert L, Desage M, Wils D, Laville M. Impact of a resistant dextrin with a prolonged oxidation pattern on day-long ghrelin profile. J Am Coll Nutr. 2011 Feb;30(1):63-72. doi: 10.1080/07315724.2011.10719945.

  PMID: 21697540 BACKGROUND

MeSH Terms

Interventions

NUTRIOSE; maltodextrin

Study Officials

• Antonio Starcevic, PhD

  University of Zagreb, Faculty of Food Technology and Biotechnology

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: DOUBLE
• Who Masked: INVESTIGATOR, OUTCOMES ASSESSOR
• Masking Details: Program is used to make random selection and grouping of participants in two groups: experimental one receiving fiber and control one, receiving placebo. A research assistant who is not involved in study (neither design nor implementation) will pack prebiotic/placebo daily doses (20 grams/day) and provide nutritionist who is one of the investigators with doses for each participant. Only when the study is completed and all results processed will the information on who received fiber/placebo be released.
• Purpose: OTHER
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Associate professor

Model Details: Double-blind study of defined prebiotic supplementation for active basketball players investigating fiber effect on microbiota composition and delayed feeling of fatigue. There are two arms, active one receiving prebiotic fibre and control one receiving placebo. Each arm contains 7 randomly selected participants who signed informed consent and will receive supplementation during 4 weeks in total while being in the same training camp and under same training conditions.

Study Record Dates

• First Submitted: July 13, 2021
• First Posted: February 14, 2023
• Study Start: September 1, 2021
• Primary Completion: October 31, 2021
• Study Completion: December 20, 2021
• Last Updated: February 14, 2023

Record last verified: 2023-02

Locations

CR (1)