Anabolic Properties of Canola

NCT05664269 | Unknown DMC

• 1 other identifier: NL81203.068.22 / METC 22-031
• Study Type: interventional
• Target: 36
• Locations: 1 country
• Sites: 1

Brief Summary

Muscle tissue consists of proteins. These proteins are built up of small building blocks: amino acids. By consuming enough protein in our diet, we make sure that the body is provided with enough amino acids to facilitate muscle protein building. Also after exercise is protein intake important as it contributes to the recovery process. Providing the growing world population with sufficient animal-derived protein is a challenge. Plant proteins can be produced on a more sustainable commercial scale than conventional animal-derived proteins and therefore, can contribute to feeding our future population. Canola protein is a protein that is derived from rapeseed. The composition of canola seems to be comparable to that of other high-quality animal-based protein sources. But there is no data yet on the effect of canola protein ingestion on muscle growth. Additionally, most research on the effect of protein intake and muscle growth/recovery has been performed in males and we need more insight into the effect in females. The goal of this study is to investigate whether the ingestion of canola protein can stimulate muscle growth just as good as whey protein after a strength exercise session in females. Primary objective: To assess the impact of 20g canola or 20g whey protein vs placebo ingestion on acute 5-hour postprandial muscle protein synthesis rates during recovery from lower-body resistance-type exercise in vivo in young females. Secondary objective: To assess the impact of 20g canola protein vs 20g whey protein ingestion on acute 5-hour postprandial muscle protein synthesis rates during recovery from lower-body resistance-type exercise in vivo in young females. Tertiary objectives: Compare signaling pathways and. postprandial 5-hour plasma glucose, insulin, and amino acid concentrations, (including area under the curve, peak concentrations, and time to peak) following canola protein, whey protein, and placebo ingestion during recovery from lower-body resistance-type exercise in young females. Hypothesis: it is hypothesized that acute 5-hour postprandial muscle protein synthesis rates will be not different following 20g canola protein and 20g whey protein ingestion and higher compared to placebo during lower-body post-exercise recovery in healthy young females.

Conditions

Muscle Protein Synthesis

Keywords

Dietary protein; resistance exercise; Plant protein; muscle metabolism

Outcome Measures

Primary Outcomes (1)

• Postprandial muscle protein fractional synthesis rate

  Muscle protein synthesis rates are calculated using L-ring-13C6-phenylalanine tracer and provided as 1 integrated value over the specified timeframe using plasma as precursor.

  0-5 hours

Secondary Outcomes (4)

• 'basal' muscle protein fractional synthesis rate

  -3 - 0 hours

• Plasma glucose concentrations

  0-5 hours

• Plasma insulin concentrations

  0-5 hours

• Plasma amino acids concentrations

  0-5 hours

Other Outcomes (10)

• Age in years

  baseline at screening

• bodymass in kg

  baseline at screening

• Height in m

  baseline at screening

Study Arms (3)

Canola protein

EXPERIMENTAL

20g of canola protein

Dietary Supplement: drink ingestion; Behavioral: resistance-type exercise

Whey protein

ACTIVE COMPARATOR

20g of whey protein

Dietary Supplement: drink ingestion; Behavioral: resistance-type exercise

Placebo

SHAM COMPARATOR

Water, matched for taste and volume

Dietary Supplement: drink ingestion; Behavioral: resistance-type exercise

Interventions

drink ingestion DIETARY_SUPPLEMENT

drink ingestion of a vanilla-flavoured beverage from a non-transparent shaker

Also known as: supplement

Canola protein; Placebo; Whey protein

resistance-type exercise BEHAVIORAL

Lower body resistance-type exercise on the supine leg press and seated knee extension machine. Both machines 4 sets at 80% 1RM for 8-10 reps.

Also known as: resistance training, strength training

Canola protein; Placebo; Whey protein

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Female sex
• Aged between 18 and 35 y inclusive
• BMI between 18.5 and 30 kg/m2

You may not qualify if:

• Intolerant to milk products
• Mustard allergy
• Participating in a structured (progressive) exercise program
• Smoking regularly
• Diagnosed GI tract disorders or diseases
• Diagnosed musculoskeletal disorders
• Diagnosed metabolic disorders (e.g. diabetes)
• Hypertension (blood pressure above 140/90 mmHg)
• Donated blood 3 months prior to test day
• Pregnant
• Using third generation oral contraceptives
• Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories).
• Chronic use of gastric acid suppressing medication
• Chronic use of anti-coagulants
• Recent (\<1 year) participation in amino acid tracer (L-\[ring-13C6\]-phenylalanine and L-\[3,5-2H2\]-tyrosine studies

Sponsors & Collaborators

• Maastricht University Medical Center: lead
• DSM Food Specialties: collaborator

Study Sites (1)

Maastricht University Medical Centre+

Maastricht, Limburg, 6229ER, Netherlands

RECRUITING

Related Publications (1)

• Boot N, Hermans WJ, Kuin LM, Malowany JM, Hendriks FK, Warnke I, Senden JM, Overman A, Goessens JP, Zorenc A, Kornips E, Verdijk LB, van Loon LJ. Ingestion of 20 g Whey or Canola Protein Does Not Further Increase Muscle Protein Synthesis Rates During Recovery From Resistance Exercise In Healthy, Young Females. J Nutr. 2025 Dec;155(12):4123-4135. doi: 10.1016/j.tjnut.2025.10.018. Epub 2025 Oct 14.

  PMID: 41101508 DERIVED

MeSH Terms

Interventions

Dietary Supplements; Resistance Training

Intervention Hierarchy (Ancestors)

Food; Diet, Food, and Nutrition; Physiological Phenomena; Food and Beverages; Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Luc van Loon, PhD

  Maastricht University

  PRINCIPAL INVESTIGATOR

Central Study Contacts

Wesley Hermans, MSc

CONTACT

0031433881810; w.hermans@maastrichtuniversity.nl

Luc van Loon, PhD

CONTACT

0031433881397; l.vanloon@maastrichtuniversity.nl

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: TRIPLE
• Who Masked: PARTICIPANT, INVESTIGATOR, OUTCOMES ASSESSOR
• Masking Details: Randomization of interventional drinks will be performed by an independent researcher who will also prepare the drinks on the morning of the test day. Drinks will be prepared in a non-transparent shaker and matched for taste.
• Purpose: BASIC SCIENCE
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: randomized, controlled, parallel-group, double-blind intervention trial.

Study Record Dates

• First Submitted: November 22, 2022
• First Posted: December 23, 2022
• Study Start: March 20, 2023
• Primary Completion: August 1, 2024
• Study Completion: August 1, 2024
• Last Updated: May 17, 2023

Record last verified: 2022-12

Data Sharing

• IPD Sharing: Will not share

Locations

NL (1)