The Relationship With Mad Honey Containing Grayanotoxin and Ovary Tissue

NCT05643599 | Completed

• 1 other identifier: MCBU_Madhoney
• Study Type: observational
• Target: 18
• Locations: 1 country
• Sites: 1

Brief Summary

For our working group, eighteen healthy Sprague-Dawley female rats were recruited and separated into three groups in an experimental animal laboratory. Group 1 was given mad honey (n:6) (80 mg/kg); Group 2 was given normal honey (n:6) (80 mg/kg), and Group 3 was the control group (n:6). The groups were given normal and mad honey by oral gavage for 30 days in this study. Rats were anesthetized intramuscularly with 50 mg/kg ketamine and 5 mg/kg xylazine on the 30th day of the study. At the conclusion of the study, female rats in the proestrus phase of the estrous cycle (as indicated by vaginal smear) were sacrificed and their ovarian tissues were placed in neutral formalin solution.

Conditions

Ovary Disease

Keywords

Grayonotoxin; Mad Honey; Normal honey; ovary

Outcome Measures

Primary Outcomes (1)

• Comparison of the Mad Honey and Normal Honey findings of the ovary tissue specimens

  Analyses of the relationship between oxidative stress and apoptosis of histopathological changes made by mad honey containing grayanotoxin in the ovary

  Baseline

Study Arms (3)

Group 1:

Mad honey

Dietary Supplement: Mad Honey

Group 2:

Normal honey

Dietary Supplement: Normal Honey

Group 3:

without intervention

Dietary Supplement: Control

Interventions

Mad Honey DIETARY_SUPPLEMENT

mad honey (80 mg/kg); (n:6)

Group 1:

Normal Honey DIETARY_SUPPLEMENT

normal honey (80 mg/kg),(n:6)

Group 2:

Control DIETARY_SUPPLEMENT

Control

Group 3:

Eligibility Criteria

• Age: 8 Weeks - 10 Weeks
• Sex: female
• Healthy Volunteers: Yes
• Age Groups: Child (0-17)
• Sampling Method: Non-Probability Sample

Study Population

experimental animals ( Sprague Dawley rats)

You may qualify if:

• healthy rats

You may not qualify if:

• patient rats
• not eating rats

Sponsors & Collaborators

• Celal Bayar University: lead

Study Sites (1)

Hayrunnisa

Yunusemre, Mani̇sa, 45030, Turkey (Türkiye)

Location

Biospecimen

Retention: SAMPLES WITH DNA

ovary tissue

MeSH Terms

Conditions

Ovarian Diseases

Condition Hierarchy (Ancestors)

Adnexal Diseases; Genital Diseases, Female; Female Urogenital Diseases; Female Urogenital Diseases and Pregnancy Complications; Urogenital Diseases; Genital Diseases; Gonadal Disorders; Endocrine System Diseases

Study Officials

• Gulsen Gurgen

  Manisa Celal Bayar University

  STUDY CHAIR

• Suha Turkmen

  Karadeniz Teknik University

  STUDY CHAIR

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Asistant Prof.

Study Record Dates

• First Submitted: November 30, 2022
• First Posted: December 8, 2022
• Study Start: December 1, 2022
• Primary Completion: December 15, 2022
• Study Completion: December 31, 2022
• Last Updated: January 27, 2025

Record last verified: 2025-01

Locations

TU (1)