Exercise-induced Changes in Exosomes

NCT05616234 | Completed

• 1 other identifier: 1956959
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

The proposed study aims to shed light on the function/importance/relevance of exercise-induced changes in exosomes to connective tissues. Exosomes are known to increase robustly in response to exercise. We have previously shown that serum isolated from subjects after they lift heavy weights increases human engineered ligament collagen content and mechanics more than serum from before they lift weight. Further, we showed that exercise-induced changes in hormones could not explain the change in ligament structure or function. These data indicate that there is a significant gap in our understanding of muscle-connective tissue crosstalk. To address this gap, the current proposal seeks to: i) isolate and sequence exosomal RNA (long non-coding, miR, and mRNA) and ii) determine whether exosomes isolated from serum after exercise increase engineered ligament mechanics and collagen content.

Conditions

Exosomes; Connective Tissue; Exercise

Outcome Measures

Primary Outcomes (1)

• Collagen content of ligament constructs

  Ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of collagen content using a hydroxyproline assay.

  15 min post-exercise

Secondary Outcomes (2)

• Ultimate tensile stress and modulus of ligament constructs

  15 min post-exercise

• Exosomal RNA

  15 min post-exercise

Study Arms (1)

Exercise trial

EXPERIMENTAL

Participants will perform a bout of exercise. In addition, a baseline and post-exercise blood sample will be drawn.

Procedure: Resistance Exercise

Interventions

Resistance Exercise PROCEDURE

Participants will perform a bout of lower body resistance exercise consisting of leg press, leg extension and leg curls.

Exercise trial

Eligibility Criteria

• Age: 18 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Young healthy adults (18-35 y)
• Experience with exercise (\>1y)

You may not qualify if:

• Pregnancy
• Smoking
• Receiving any medication that may interfere with the study outcomes

Sponsors & Collaborators

• University of California, Davis: lead

Study Sites (1)

Hickey Laboratory

Davis, California, 95616, United States

Location

MeSH Terms

Conditions

Motor Activity

Interventions

Resistance Training

Condition Hierarchy (Ancestors)

Behavior

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Study Officials

• Keith Baar, PhD

  University of California, Davis

  PRINCIPAL INVESTIGATOR

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: TREATMENT
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: November 7, 2022
• First Posted: November 15, 2022
• Study Start: November 7, 2022
• Primary Completion: February 17, 2023
• Study Completion: February 17, 2023
• Last Updated: June 15, 2023

Record last verified: 2023-06

Locations

US (1)