Impact of Acute Exercise Intensity and Pattern on Cytokine Function
AEX
1 other identifier
interventional
16
1 country
1
Brief Summary
The immune system helps prevent illness, fights off infections, and repairs damaged tissues following an injury. However, when immune cells remain active for prolonged periods of time - a state known as "chronic inflammation" - they can contribute to the development and progression of chronic diseases like heart disease and diabetes. Exercise can reduce the risk of developing many of these diseases and at least part of the health benefits of exercise are due to the ability of exercise to reduce "chronic inflammation". The inflammation-lowering effects of exercise are typically captured by measuring hormone-like molecules released from immune cells called "cytokines" in the blood. In addition to changes in circulating cytokine levels, exercise may also alter how immune cells respond to these cytokines. How exercise intensity (i.e., how hard you are working during exercise) and pattern (i.e., exercising as a long continuous bout or in short intervals) impact the ability of immune cells to respond to cytokines is not well understood. A better understanding of how exercise intensity and pattern of exercise for reducing chronic inflammation may help determine the best types of exercises for improving health and preventing chronic diseases.
Trial Health
Trial Health Score
Automated assessment based on enrollment pace, timeline, and geographic reach
participants targeted
Target at below P25 for not_applicable
Started Sep 2022
1 active site
Health score is calculated from publicly available data and should be used for screening purposes only.
Trial Relationships
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Study Timeline
Key milestones and dates
Study Start
First participant enrolled
September 15, 2022
CompletedFirst Submitted
Initial submission to the registry
October 6, 2022
CompletedFirst Posted
Study publicly available on registry
October 10, 2022
CompletedPrimary Completion
Last participant's last visit for primary outcome
August 1, 2023
CompletedStudy Completion
Last participant's last visit for all outcomes
September 1, 2023
CompletedDecember 6, 2023
December 1, 2023
11 months
October 6, 2022
December 4, 2023
Conditions
Keywords
Outcome Measures
Primary Outcomes (1)
IL-10 mediated STAT3 phosphorylation
Ex vivo leukocyte STAT3 phosphorylation in response to IL-10 treatment
Change from pre-exercise to immediately and 90-min post-exercise
Secondary Outcomes (8)
IL-10 mediated TNF-alpha inhibition
Change from pre-exercise to immediately and 90-min post-exercise
IL-6 mediated STAT3 phosphorylation
Change from pre-exercise to immediately and 90-min post-exercise
IL-6 mediated TNF-alpha inhibition
Change from pre-exercise to immediately and 90-min post-exercise
Plasma IL-10
Change from pre-exercise to immediately, 30-, and 90-min post-exercise
Plasma IL-6
Change from pre-exercise to immediately, 30-, and 90-min post-exercise
- +3 more secondary outcomes
Study Arms (4)
Resting (no exercise) control
PLACEBO COMPARATORResting (no exercise) control condition
Moderate intensity continuous exercise (MICE)
EXPERIMENTALExperimental session involving an acute bout of moderate intensity continuous exercise (MICE; continous cycling expending 350 kcal at 70% of lactate threshold)
High intensity continuous exercise (HICE)
EXPERIMENTALExperimental session involving an acute bout of high intensity continuous exercise (HICE; continuous cycling expending 350 kcal at 10% of the difference between lactate threshold and VO2peak)
High intensity interval exercise (HIIT)
EXPERIMENTALExperimental session involving an acute bout of high intensity interval exercise (HIIT; cycling intervals expending 350 kcal at 10% of the difference between lactate threshold and VO2peak)
Interventions
Participants will perform an acute bout of continuous cycling at 70% of the power output at lactate threshold until an energy expenditure of 350 kcal is achieved. Blood samples will be obtained immediately before and immediately, 30, and 90 minutes after exercise.
Participants will perform an acute bout of continuous cycling at 10% of the difference between lactate threshold and VO2peak until an energy expenditure of 350 kcal is achieved. Blood samples will be obtained immediately before and immediately, 30, and 90 minutes after exercise.
Participants will perform an acute bout of interval cycling at at 10% of the difference between lactate threshold and VO2peak until an energy expenditure of 350 kcal is achieved. Blood samples will be obtained immediately before and immediately, 30, and 90 minutes after exercise.
Participants will remain in a rested state (i.e., no exercise) for the entire session. Blood samples will be obtained at the same time-points as the exercise sessions.
Eligibility Criteria
You may qualify if:
- years of age
- Body mass index between 18.5-30 kg/m\^2
- Free of cardiometabolic and autoimmune/inflammatory disease
You may not qualify if:
- Competitive endurance athlete
- Cigarette smoker
- Currently taking immunomodulatory/anti-inflammatory medications
- Currently pregnant
Contact the study team to confirm eligibility.
Sponsors & Collaborators
Study Sites (1)
UBC Okanagan
Kelowna, British Columbia, V1V1V7, Canada
MeSH Terms
Conditions
Interventions
Condition Hierarchy (Ancestors)
Intervention Hierarchy (Ancestors)
Study Officials
- PRINCIPAL INVESTIGATOR
Jonathan P Little, PhD
UBC Okanagan
Study Design
- Study Type
- interventional
- Phase
- not applicable
- Allocation
- RANDOMIZED
- Masking
- NONE
- Purpose
- BASIC SCIENCE
- Intervention Model
- CROSSOVER
- Sponsor Type
- OTHER
- Responsible Party
- PRINCIPAL INVESTIGATOR
- PI Title
- Professor
Study Record Dates
First Submitted
October 6, 2022
First Posted
October 10, 2022
Study Start
September 15, 2022
Primary Completion
August 1, 2023
Study Completion
September 1, 2023
Last Updated
December 6, 2023
Record last verified: 2023-12