NCT05526885

Brief Summary

According to WHO, about 40% of the incident TB cases in 2020 are either under-reported or under-diagnosed causing on one hand major health risks and on the other hand catastrophic financial consequences. In particular, indigent people in hard-to-reach communities with high TB/HIV burden are at high risk of missed or delayed diagnoses. Hence, active case finding for TB remains an integral part of tuberculosis control in high-risk groups, such as people living with HIV (PLHIV) or diabetes mellitus, people living in specific geographical locations associated with a high burden of TB and poor access to health care, miners, or prisoners. CAD4TB (Delft Imaging, NL), a digital chest X-ray analysis software, and point-of care C-reactive protein assay (POC-CRP; e.g. LumiraDx, UK), which detects a cytokine induced acute phase protein, are two tests which have great potential of becoming a screening and triage test for TB as outlined in the WHO target product profiles. Data on CAD4TB and CRP suggest that accuracy can be improved if thresholds are stratified by patient characteristics, such as HIV status, history of TB and TB symptoms. TB TRIAGE+ Trial takes place in the communities of Lesotho and South Africa, which present high prevalence of subclinical TB, where a symptom-based screening would miss almost half of all infectious TB cases. TB TRIAGE+ Trials conducts a direct (in the same individual) comparison of the two screening/triaging approaches which are not based on symptoms: CAD4TB screening alone (approach 1) versus CAD4TB screening with POC-CRP triage testing (approach 2), and followed by confirmatory Xpert MTB/RIF Ultra testing in both approaches. TB TRIAGE TRIAL is investigates the hypothesis that a community-based active case finding strategy with CAD4TB screening with POC-CRP triage testing (approach 2) will be non-inferior compared to CAD4TB screening alone (approach 1) with regard to yield of detected TB cases and superior with regard to cost effectiveness.

Trial Health

90
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
20,000

participants targeted

Target at P75+ for not_applicable

Timeline
Completed

Started Sep 2022

Typical duration for not_applicable

Geographic Reach
2 countries

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

August 31, 2022

Completed
2 days until next milestone

First Posted

Study publicly available on registry

September 2, 2022

Completed
5 days until next milestone

Study Start

First participant enrolled

September 7, 2022

Completed
2.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

December 10, 2024

Completed
21 days until next milestone

Study Completion

Last participant's last visit for all outcomes

December 31, 2024

Completed
Last Updated

September 22, 2025

Status Verified

September 1, 2025

Enrollment Period

2.3 years

First QC Date

August 31, 2022

Last Update Submit

September 16, 2025

Conditions

Keywords

LesothoSouth Africacommunity-basedtuberculosisactive case findingscost-effectivenessCAD4TBPOC-CRPXpert MTB/RIF Ultratriagescreening

Outcome Measures

Primary Outcomes (2)

  • Yield of detected TB cases per study approach: number of positive Xpert MTB/RIF Ultra results per screening approach.

    24 Months

  • Cost-effectiveness of the CAD4TBv7-CRP approach using the CAD4TBv7 approach as a comparator: the costs for each positive Xpert Ultra case detected in the CAD4TBv7-CRP approach compared to the CAD4TBv7 approach

    24 Months

Secondary Outcomes (4)

  • Extension of primary outcome: Cost-effectiveness of the CAD4TBv7-CRP approach and the CAD4TBv7 approach compared to passive case finding: cost per TB case detected

    24 Months

  • Equity analysis: 1. Socioeconomic status of households with actively detected TB cases compared to those with passively detected TB cases and to households with no TB cases: wealth quintiles distribution, income distribution and main source of income

    24 Months

  • Equity analysis: 2. Average out of pocket payments (OOP) associated with accessing TB treatment for households with identified TB cases across different wealth quintiles

    24 months

  • Health impact: The expected number of identified TB cases via passive case finding if the active case finding campaign had not taken place will be compared to the number of cases identified in the campaign

    24 months

Study Arms (2)

Approach 1 - CAD4TB screening

EXPERIMENTAL

Participants with a CAD4TB version 7 (Delft Imaging, NL) score above the overall threshold (as defined for study approach 1) are eligible for Xpert MTB/RIF Ultra testing.

Diagnostic Test: CAD4TBDiagnostic Test: Xpert MTB/RIF Ultra

Approach 2 - CAD4TB screening with POC-CRP triage testing

EXPERIMENTAL

Participants with a CAD4TB version 7 (Delft Imaging, NL) score within the threshold window (between lower and upper limit threshold as defined for study approach 2), a POC-CRP LumiraDx (LumiraDx Limited, UK) test will follow as a triage test. If CRP is above the determined threshold as defined for approach 2, Xpert MTB/RIF Ultra will be performed. If the CAD4TB score is above the upper limit threshold as defined for approach 2, Xpert MTB/RIF Ultra will be performed (without further CRP testing).

Diagnostic Test: CAD4TBDiagnostic Test: POC-CRPDiagnostic Test: Xpert MTB/RIF Ultra

Interventions

CAD4TBDIAGNOSTIC_TEST

A posterior anterior digital chest X-ray incoporated in a mobile diagnostic unit is analysed by CAD4TB version 7 (Delft Imaging, NL). A trained and qualified person in compliance with national regulations will operate the digital chest X-ray.

Approach 1 - CAD4TB screeningApproach 2 - CAD4TB screening with POC-CRP triage testing
POC-CRPDIAGNOSTIC_TEST

A quantitative POC-CRP LumiraDx (LumiraDx Limited, UK) triage test will be performed by finger-prick if CAD4TB scores is within the defined threshold window of study approach 2 triage testing

Approach 2 - CAD4TB screening with POC-CRP triage testing
Xpert MTB/RIF UltraDIAGNOSTIC_TEST

Depending on CAD4TB (approach 1 \& 2) or POC-CRP score (approach 2) a confirmatory Xpert MTB/RIF Ultra(Cepheid, USA) rapid sputum molecular testing follows for both Mycobacterium tuberculosis complex and rifampicin resistance;

Approach 1 - CAD4TB screeningApproach 2 - CAD4TB screening with POC-CRP triage testing

Eligibility Criteria

Age18 Years+
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Willing and able to provide signed written consent or witnessed oral consent in the case of illiteracy, prior to undertaking any study-related procedure
  • Adults (≥18 years)

You may not qualify if:

  • Any condition for which participation in the study, as judged by the investigator or a designated staff member, could compromise the well-being of the subject or prevent, limit or confound protocol specified assessments
  • Seriously ill person who needs immediate medical care
  • Current anti-TB treatment (preventive TB treatment permitted)
  • Lesotho only: Pregnancy (self-reported)

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

SolidarMed

Maseru, Lesotho

Location

Human Sciences Research Council

Pietermaritzburg, KwaZulu-Natal, South Africa

Location

Related Publications (2)

  • Signorell A, van Heerden A, Ayakaka I, Jacobs BK, Antillon M, Tediosi F, Verjans A, Brugger C, Harkare HV, Labhardt ND, Bosman S, Kamele M, Keitseng M, Madonsela T, Kurscheid J, Muhairwe J, Keter AK, Murphy K, van Ginneken B, Gils T, Katende B, Gebresenbet RF, Erhardt RM, Zoller T, Vanobberghen F, Glass TR, Lynen L, Reither K. Effectiveness and cost-effectiveness of community-based TB screening algorithms using computer-aided detection (CAD) technology alone compared with CAD combined with point-of-care C reactive protein testing in Lesotho and South Africa: protocol for a paired screen-positive trial. BMJ Open. 2025 Jul 28;15(7):e093989. doi: 10.1136/bmjopen-2024-093989.

  • Pita TP, Misra S, Madonsela T, Tshazi A, Bosman S, Ayakaka I, Vlieghe E, Decroo T, Reither K, van Heerden A, Gils T. "If your CD4 count lowers, that is when you are similar to a person that is non-existent" A qualitative exploration of perceptions around advanced HIV disease in South Africa. BMC Public Health. 2025 Jul 3;25(1):2325. doi: 10.1186/s12889-025-23588-1.

Related Links

MeSH Terms

Conditions

Tuberculosis

Condition Hierarchy (Ancestors)

Mycobacterium InfectionsActinomycetales InfectionsGram-Positive Bacterial InfectionsBacterial InfectionsBacterial Infections and MycosesInfections

Study Design

Study Type
interventional
Phase
not applicable
Allocation
NON RANDOMIZED
Masking
NONE
Purpose
DIAGNOSTIC
Intervention Model
SINGLE GROUP
Model Details: In this study a paired screen-positive design is used to directly compare two screening/triage approaches in the same individual based on a paired analysis: CAD4TB screening only (approach 1) versus CAD4TB screening with POC-CRP triage algorithm (approach 2).
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
Head of Clinical Research Unit

Study Record Dates

First Submitted

August 31, 2022

First Posted

September 2, 2022

Study Start

September 7, 2022

Primary Completion

December 10, 2024

Study Completion

December 31, 2024

Last Updated

September 22, 2025

Record last verified: 2025-09

Data Sharing

IPD Sharing
Will share

All data will be stored securely in a way that allows future access for the research team. We will adhere to the FAIR Guiding Principles for data management and stewardship. After completion of the studies and after anonymization (e.g. replace subject identifier with a new random subject identifier), analysis datasets will be made publicly available in a timely manner on a non-commercial data platform according to the FAIR principles.

Locations