Regulatory Network and Diagnostic Value of Key Autophagy-related Genes in Sepsis

NCT05524376 | Unknown

• 1 other identifier: SYSKY-2022-251-01
• Study Type: observational
• Target: 40
• Locations: 0 countries
• Sites: N/A

Brief Summary

Autophagy plays an important role in the occurrence and development of sepsis. This study aims to explore and verify the key autophagy-related genes in sepsis, then construct their regulatory networks and evaluate their potential diagnostic value, so as to provide new ideas for the diagnosis and treatment of sepsis.

Conditions

Sepsis; Autophagy

Keywords

sepsis; autophagy

Outcome Measures

Primary Outcomes (3)

• Diagnostic Value of Key Autophagy-related Genes in Sepsis

  The diagnostic functions of key DEARGs (Differential Autophagy-related Genes) were analyzed

  October 2022

• Regulatory Network of Key Autophagy-related Genes in Sepsis

  Key DEARGs in sepsis were analyzed for transcription factors and ceRNA regulatory network

  October 2022

• Verified Key Autophagy-related Genes in Sepsis by RT-qPCR.

  Peripheral blood samples from sepsis patients and healthy controls were collected and verified by RT-qPCR.

  December 2022

Study Arms (2)

sepsis group

Other: no intervention

control group

Other: no intervention

Interventions

no intervention OTHER

no intervention

control group; sepsis group

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

sepsis group: Eligible patients with sepsis admitted to the ICU of Sun Yat-sen Memorial Hospital of Sun Yat-sen University from September 2022 to December 2022. control group: Age - and sex-matched healthy controls.

Sponsors & Collaborators

• Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University: lead

Related Publications (4)

• Singer M, Deutschman CS, Seymour CW, Shankar-Hari M, Annane D, Bauer M, Bellomo R, Bernard GR, Chiche JD, Coopersmith CM, Hotchkiss RS, Levy MM, Marshall JC, Martin GS, Opal SM, Rubenfeld GD, van der Poll T, Vincent JL, Angus DC. The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA. 2016 Feb 23;315(8):801-10. doi: 10.1001/jama.2016.0287.

  PMID: 26903338 BACKGROUND

• Takahashi W, Watanabe E, Fujimura L, Watanabe-Takano H, Yoshidome H, Swanson PE, Tokuhisa T, Oda S, Hatano M. Kinetics and protective role of autophagy in a mouse cecal ligation and puncture-induced sepsis. Crit Care. 2013 Jul 24;17(4):R160. doi: 10.1186/cc12839.

  PMID: 23883625 BACKGROUND

• Aguirre A, Lopez-Alonso I, Gonzalez-Lopez A, Amado-Rodriguez L, Batalla-Solis E, Astudillo A, Blazquez-Prieto J, Fernandez AF, Galvan JA, dos Santos CC, Albaiceta GM. Defective autophagy impairs ATF3 activity and worsens lung injury during endotoxemia. J Mol Med (Berl). 2014 Jun;92(6):665-76. doi: 10.1007/s00109-014-1132-7. Epub 2014 Feb 19.

  PMID: 24535031 BACKGROUND

• Oami T, Watanabe E, Hatano M, Sunahara S, Fujimura L, Sakamoto A, Ito C, Toshimori K, Oda S. Suppression of T Cell Autophagy Results in Decreased Viability and Function of T Cells Through Accelerated Apoptosis in a Murine Sepsis Model. Crit Care Med. 2017 Jan;45(1):e77-e85. doi: 10.1097/CCM.0000000000002016.

  PMID: 27618275 BACKGROUND

MeSH Terms

Conditions

Sepsis

Condition Hierarchy (Ancestors)

Infections; Systemic Inflammatory Response Syndrome; Inflammation; Pathologic Processes; Pathological Conditions, Signs and Symptoms

Central Study Contacts

Zhijie He

CONTACT

13710898290; hezhijie2004@126.com

Li Yang

CONTACT

15254586586; 375948656@qq.com

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: August 30, 2022
• First Posted: September 1, 2022
• Study Start: September 1, 2022
• Primary Completion: December 31, 2022
• Study Completion: December 31, 2022
• Last Updated: September 1, 2022

Record last verified: 2022-08