NCT05482321

Brief Summary

The overall goal of this study is to develop and test a novel method involving ultrasound imaging, in order to detect the development of type 1 diabetes. In this study the investigators will first establish a standard operating procedure for measuring pancreas blood flow speed and volume in the pancreas of human subjects. The investigators will then determine 1) whether these pancreas blood flow factors differ between healthy subjects and those who have recently developed type1 diabetes; and 2) how variable measurements are in healthy subjects and subjects that recently developed type1 diabetes, both between subjects and over time. To address these aims the investigators will perform pancreas ultrasound measurements in each subject using an approved injectable 'bubble' contrast agent that allows measurement of pancreas blood flow. The investigators will compare ultrasound measurement with characteristics of the subject's type 1 diabetes, including genetic factors, glucose levels and other circulating factors, as well as other factors that may influence blood flow in the pancreas independent of type1 diabetes. The successful conclusion of this study will indicate whether measuring pancreas blood flow speed/volume will be helpful in monitoring whether type1 diabetes will emerge and thus will allow a large scale study to answer this question.

Trial Health

77
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
50

participants targeted

Target at P25-P50 for all trials

Timeline
4mo left

Started Feb 2023

Typical duration for all trials

Geographic Reach
1 country

1 active site

Status
recruiting

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

Study Progress91%
Feb 2023Sep 2026

First Submitted

Initial submission to the registry

July 28, 2022

Completed
4 days until next milestone

First Posted

Study publicly available on registry

August 1, 2022

Completed
7 months until next milestone

Study Start

First participant enrolled

February 27, 2023

Completed
3.5 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 1, 2026

Expected
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

September 1, 2026

Last Updated

May 6, 2026

Status Verified

May 1, 2026

Enrollment Period

3.5 years

First QC Date

July 28, 2022

Last Update Submit

May 4, 2026

Conditions

Keywords

ultrasoundtissue perfusioncontrastdiabetespancreas

Outcome Measures

Primary Outcomes (4)

  • Primary outcome 1

    Optimized a contrast-enhanced ultrasound 'destruction-replenishment' protocol for imaging pancreas blood flow kinetics in adult human subjects.

    End of part I (6 months)

  • Primary outcome 2

    Comparison of pancreas blood flow kinetics (i.e. 'destruction-replenishment' k2 'reperfusion rate' parameter) between control and T1D subjects.

    End of part II (1 year)

  • Primary outcome 3

    Determining inter-subject variability in pancreas blood flow kinetics (i.e. the 'destruction-replenishment' k2 'reperfusion rate' parameter) among control subjects and among T1D subjects

    End of part II (1 year)

  • Primary outcome 4

    Determining reproducibility in the measurement of pancreas blood flow kinetics (i.e. the 'destruction-replenishment' k2 'reperfusion rate' parameter) within subjects. This will initially focus on short-term intra-subject measurement variability.

    End of part II (1 year)

Secondary Outcomes (6)

  • Secondary outcome 1

    End of part II (1 year)

  • Secondary outcome 2

    End of part II (1 year)

  • Secondary outcome 3

    End of part II (1 year)

  • Secondary outcome 4

    End of part II (1 year)

  • Secondary outcome 5

    End of part II (1 year)

  • +1 more secondary outcomes

Study Arms (3)

Group 1

SOP (healthy) subjects. Part I of study (optimizing protocol).

Drug: Definity Suspension for Injection

Group 2

Control (healthy and autoantibody positive) subjects. Part II of study (cross-sectional study)

Drug: Definity Suspension for Injection

Group 3

T1D subjects. Part II of study (cross-sectional study)

Drug: Definity Suspension for Injection

Interventions

* DEFINITY is administered as per manufactures instructions * 1 vial of DEFINITY is allowed to warm to room temperature and activated by 45s shaking on a VIALMIX * Withdraw the activated milky white suspension using the provided Dispensing Pin, PINSYNC or 18-20 gauge syringe needle, from the middle of the liquid in the inverted vial. * Deliver a dose of 10 μl/kg of 'milky white suspension' IV as a bolus, delivered over 30seconds. * Following imaging, provide a 10 mL flush of preservative-free saline * Wait at least 30minutes until second dose (if provided).

Also known as: DEFINITY
Group 1Group 2Group 3

Eligibility Criteria

Age18 Years - 65 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)
Sampling MethodProbability Sample
Study Population

Group 1 subjects will be healthy subjects who will receive contrast-enhanced ultrasound 'destruction-replenishment' measurements, in order to optimize and establish the standard operating procedure (SOP) for this measurement in the pancreas of human subjects. This SOP will then be applied to Group 2 (healthy subjects and autoantibody positive subjects) and Group 3 (recent onset type1 diabetes) subjects as part of the cross-sectional study. Classification of type 1 diabetes includes meeting ADA criteria for diabetes, within 180 days of diagnosis, and evidence of at least 1 islet-associated autoantibody.

You may qualify if:

  • Male or non-pregnant female age 18-65
  • Ability and willingness of patient to participate fully in all aspects of this clinical study
  • Written informed consent obtained and documented

You may not qualify if:

  • Excessive body size preventing effective scan of the pancreas as determined by sonographer
  • Evidence of exocrine pancreatic disease, including pancreatitis, cystic fibrosis, pancreatic adenocarcinoma, or neuroendocrine tumor.
  • Subjects who are pregnant or breast-feeding
  • Subjects incapable of giving assent/informed written consent
  • Known or suspected hypersensitivity to perflutren
  • Known history or suspected unstable cardiopulmonary conditions (acute myocardial infarction, acute coronary artery syndromes, worsening or unstable congestive heart failure, or serious ventricular arrhythmias)
  • Group 2 (control subjects, part II):
  • Diagnosis of diabetes according to the ADA recommended criteria (blood glucose and HbA1c will be measured)
  • Use of medications used to control high blood sugar (GLP1R agonists, metformin\*, sulfonylureas)
  • \*Participants taking metformin will be considered eligible if they are willing/able to stop taking the medication for at least two weeks prior to each scan.
  • Any person who has received immunomodulatory intervention within 3 months of enrollment
  • Group 3 (T1D subjects, part II)
  • Diagnosis of diabetes according to the ADA recommended criteria (blood glucose and HbA1c will be measured)
  • Evidence of autoantibodies to at least 1 of the following β-cell autoantigens:
  • insulin, IA-2, GAD65, ZnT8
  • +5 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

University of Colorado Anschutz, Barbara Davis Center

Aurora, Colorado, 80045, United States

RECRUITING

Biospecimen

Retention: SAMPLES WITH DNA

Blood sample

MeSH Terms

Conditions

PancreatitisDiabetes Mellitus

Interventions

Injectionsperflutren

Condition Hierarchy (Ancestors)

Pancreatic DiseasesDigestive System DiseasesGlucose Metabolism DisordersMetabolic DiseasesNutritional and Metabolic DiseasesEndocrine System Diseases

Intervention Hierarchy (Ancestors)

Drug Administration RoutesDrug TherapyTherapeutics

Study Officials

  • Richard KP Benninger, PhD

    University of Colorado, Denver

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
observational
Observational Model
CASE CONTROL
Time Perspective
CROSS SECTIONAL
Target Duration
1 Year
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

July 28, 2022

First Posted

August 1, 2022

Study Start

February 27, 2023

Primary Completion (Estimated)

September 1, 2026

Study Completion (Estimated)

September 1, 2026

Last Updated

May 6, 2026

Record last verified: 2026-05

Data Sharing

IPD Sharing
Will not share

Locations