NCT05454943

Brief Summary

This study will evaluate the implementation and effectiveness of free-living TRE on biological and behavioural breast cancer risk factors.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
178

participants targeted

Target at P75+ for not_applicable

Timeline
Completed

Started Jul 2022

Geographic Reach
1 country

1 active site

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

July 7, 2022

Completed
Same day until next milestone

Study Start

First participant enrolled

July 7, 2022

Completed
5 days until next milestone

First Posted

Study publicly available on registry

July 12, 2022

Completed
1.2 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

September 20, 2023

Completed
4 months until next milestone

Study Completion

Last participant's last visit for all outcomes

January 30, 2024

Completed
Last Updated

October 1, 2025

Status Verified

September 1, 2025

Enrollment Period

1.2 years

First QC Date

July 7, 2022

Last Update Submit

September 26, 2025

Conditions

Outcome Measures

Primary Outcomes (2)

  • Adherence to TRE for the 16-week intervention

    Assessed daily through twice daily automated text messages asking participants to respond with what time they started and stopped eating that day.

    16-week average

  • Hemoglobin A1c

    Collected via venipuncture

    16 Weeks

Secondary Outcomes (20)

  • HOMA-IR

    16 Weeks

  • Free-living glucose control

    16 Weeks

  • C-Reactive Protein

    16 Weeks

  • Lipid profile

    16 Weeks

  • Body weight

    16 Weeks

  • +15 more secondary outcomes

Other Outcomes (2)

  • Anxiety

    16 weeks

  • Depression

    16 weeks

Study Arms (5)

Standardized TRE protocol + External Support

EXPERIMENTAL

Participants will be asked to follow a standardized 16:8 TRE protocol with an 11 am to 7 pm eating window for 16 weeks. Participants receive support to follow the TRE intervention from the study team which will include an initial consultation with a registered dietitian and ongoing tailored feedback via brief support calls from study staff at weeks 1, 3, 6, and 12.

Behavioral: Standardized TRE ProtocolBehavioral: External Support for TRE

Standardized TRE protocol + Peer Support

EXPERIMENTAL

Participants will be asked to follow a standardized 16:8 TRE protocol with an 11 am to 7 pm eating window for 16 weeks. Each participant will be matched with another participant in the same group to provide peer-based support to adhere to TRE. Study staff will introduce pairs and provide an introduction to TRE and then pairs will meet by phone/video at weeks 1, 3, 6, and 12 at a minimum, with additional contact encouraged.

Behavioral: Standardized TRE ProtocolBehavioral: Peer Support for TRE

Personalized TRE protocol + External Support

EXPERIMENTAL

Participants will be asked to follow TRE for 16 weeks with a protocol that is personalized to their preferences within the following pre-specified rules: 1) 8-10 hour eating window; 2) self-selected eating window start time as long as it ends ≥3h before bedtime; 3) if days off are required, aim to do so after following TRE for ≥5 successive days each week. Participants receive support to follow the TRE intervention from the study team which will include an initial consultation with a registered dietitian and ongoing tailored feedback via brief support calls from study staff at weeks 1, 3, 6, and 12.

Behavioral: Personalized TRE ProtocolBehavioral: External Support for TRE

Personalized TRE protocol + Peer Support

EXPERIMENTAL

Participants will be asked to follow TRE for 16 weeks with a protocol that is personalized to their preferences within the following pre-specified rules: 1) 8-10 hour eating window; 2) self-selected eating window start time as long as it ends ≥3h before bedtime; 3) if days off are required, aim to do so after following TRE for ≥5 successive days each week. Each participant will be matched with another participant in the same group to provide peer-based support to adhere to TRE. Study staff will introduce pairs and provide an introduction to TRE and then pairs will meet by phone/video at weeks 1, 3, 6, and 12 at a minimum, with additional contact encouraged.

Behavioral: Personalized TRE ProtocolBehavioral: Peer Support for TRE

Control Group

NO INTERVENTION

Participants will be asked to maintain their usual diet and physical activity patterns for 16 weeks. They will receive the same number and timing of calls from study staff as the other groups (at randomization, 1, 3, 6, 12 weeks).

Interventions

A standardized 16:8 TRE protocol with an 11 am to 7 pm eating window.

Standardized TRE protocol + External SupportStandardized TRE protocol + Peer Support

A personalized TRE protocol following pre-specified rules: 1) 8-10 hour eating window; 2) self-selected eating window start time as long as it ends ≥3h before bedtime; 3) if days off are required, aim to do so after following TRE for ≥5 successive days each week.

Personalized TRE protocol + External SupportPersonalized TRE protocol + Peer Support

External support (from study staff) that includes consultation with a registered dietitian and ongoing tailored feedback via support calls at weeks 1, 3, 6, and 12.

Personalized TRE protocol + External SupportStandardized TRE protocol + External Support

Matched pairs will meet by phone/video at weeks 1, 3, 6, and 12.

Personalized TRE protocol + Peer SupportStandardized TRE protocol + Peer Support

Eligibility Criteria

Age50 Years+
Sexfemale
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • Females
  • No cancer history
  • ≥50 years old
  • Have metabolic dysfunction (defined as self-reported diagnosis of pre-diabetes, type 2 diabetes, or at least moderate Canadian diabetes risk (CANRISK) score)
  • Access to an Ontario Lifelabs location
  • Owns a smartphone with Bluetooth capability with Apple or Android operating system

You may not qualify if:

  • Type 1 diabetes
  • Taking exogenous insulin, sulfonylureas, or GLP-1 receptor agonists
  • Self-reported history of an eating disorder
  • BMI \<18.5 kg/m
  • Working night or rotating shifts
  • Eating window \<12 hours or consistently eating less than 3 meals/day in the past 3 months
  • Major dietary changes within the past 3 months (i.e. calorie counting, ketogenic diet)

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Remote Ontario-wide

Toronto, Ontario, Canada

Location

MeSH Terms

Interventions

Trehalase

Intervention Hierarchy (Ancestors)

DisaccharidasesGlycoside HydrolasesHydrolasesEnzymesEnzymes and Coenzymes

Study Officials

  • Amy A Kirkham, PhD

    University of Toronto

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
SINGLE
Who Masked
OUTCOMES ASSESSOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
PRINCIPAL INVESTIGATOR
PI Title
Amy Kirkham, Assistant Professor

Study Record Dates

First Submitted

July 7, 2022

First Posted

July 12, 2022

Study Start

July 7, 2022

Primary Completion

September 20, 2023

Study Completion

January 30, 2024

Last Updated

October 1, 2025

Record last verified: 2025-09

Data Sharing

IPD Sharing
Will not share

Locations