NCT05446740

Brief Summary

The purpose of this first-time-in-human (FTiH) study is to assess the safety, reactogenicity and immunogenicity of GlaxoSmithKline's (GSK) messenger RNA (mRNA)-based monovalent vaccine (GSK4382276A) candidate against influenza in healthy younger adults (YA) and older adults (OA).

Trial Health

90
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
324

participants targeted

Target at P75+ for phase_1

Timeline
Completed

Started Aug 2022

Geographic Reach
3 countries

7 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

June 28, 2022

Completed
9 days until next milestone

First Posted

Study publicly available on registry

July 7, 2022

Completed
1 month until next milestone

Study Start

First participant enrolled

August 9, 2022

Completed
1.6 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

March 26, 2024

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

March 26, 2024

Completed
2 years until next milestone

Results Posted

Study results publicly available

March 10, 2026

Completed
Last Updated

March 10, 2026

Status Verified

February 1, 2026

Enrollment Period

1.6 years

First QC Date

June 28, 2022

Results QC Date

September 26, 2025

Last Update Submit

February 17, 2026

Conditions

Keywords

InfluenzaSafetyReactogenicityImmunogenicitymRNA vaccineHealthy younger adultsHealthy older adults

Outcome Measures

Primary Outcomes (12)

  • Number of Participants Reporting Any Solicited Administration Site Events

    Assessed solicited administration site events included pain, erythema/redness, swelling and Lymphadenopathy (defined as localized axillary, cervical or supraclavicular swelling or tenderness ipsilateral to the injection arm). Any = occurrence of the event regardless of intensity grade.

    Day 1 to Day 7

  • Number of Participants Reporting Any Solicited Systemic Events

    Assessed solicited systemic events included fever, chills, headache, myalgia, arthralgia and fatigue. Any = occurrence of the symptom regardless of intensity grade.

    Day 1 to Day 7

  • Number of Participants Reporting Any Unsolicited Adverse Events (AEs)

    An unsolicited AEs is an AEs that was either not included in the list of solicited events or could be included in the list of solicited events but with an onset outside the specified period of follow up for solicited events. Unsolicited AEs must have been communicated by a participant who has signed the informed consent or through his/her caregiver. Unsolicited AEs include both serious and non-serious AEs. Any = occurrence of the symptom regardless of intensity grade or relation to study vaccination.

    Day 1 to Day 28

  • Number of Participants Reporting Serious Adverse Events (SAEs)

    An SAE is defined as any untoward medical occurrence that resulted in death, was life-threatening, required inpatient hospitalization or prolongation of existing hospitalization, resulted in disability/incapacity, was a congenital anomaly/birth defect in the offspring of a study participant, or resulted in abnormal pregnancy outcomes, or in other situations that were considered serious per medical or scientific judgment.

    Day 1 to Day 183

  • Number of Participants Reporting AEs of Special Interest (AESIs)

    The following events were considered as AESI in this study: severe hypersensitivity reactions within 24 hours after study intervention administration, myocarditis/pericarditis and potential immune-mediated diseases (pIMDs).

    Day 1 to Day 183

  • Number of Participants Reporting Shift From Abnormal Non-clinically Significant and Normal or Missing Laboratory Value on Day 1 to Clinically Significant Abnormal Laboratory Value on Day 8 for Hematology, Clinical Chemistry, Coagulation and Urine Analysis

    Clinically significant abnormal laboratory findings are those which are not associated with an underlying disease, unless judged by the investigator to be more severe than expected for the participants condition. Normal or missing values refer to laboratory values that were within normal range or missing at baseline.

    At Day 8 compared to baseline (Day 1)

  • Number of Participants Reporting Shift From Abnormal Non-clinically Significant and Normal or Missing Laboratory Value on Day 1 to Clinically Significant Abnormal Laboratory Value on Day 29 for Hematology,Clinical Chemistry, Coagulation and Urine Analysis

    Clinically significant abnormal laboratory findings are those which are not associated with an underlying disease, unless judged by the investigator to be more severe than expected for the participants condition. Normal or missing values refer to laboratory values that were within normal range or missing at baseline.

    At Day 29 compared to baseline (Day 1)

  • Geometric Mean Titers (GMT) of Anti-vaccine Antibody Titers

    At Day 1

  • GMT of Anti-vaccine Antibody Titers

    At Day 22

  • Geometric Mean Increase (GMI) of Anti-vaccine Antibody Titers From Day 1 (Baseline) to Day 22

    GMI is defined as the geometric mean of the ratios of the post-dose anti-vaccine antibody titers over the Day 1 anti-vaccine antibody titers.

    From Day 1 to Day 22

  • Percentage of Participants With Anti-vaccine Antibody Seroconversion Rate (SCR)

    SCR is defined as the percentage of dosed participants who have either an anti-vaccine antibody pre-dose titer \< 1:10 and a post-dose anti-vaccine antibody titer ≥ 1:40 or a pre-dose anti-vaccine antibody titer ≥ 1:10 and at least a 4-fold increase in post-dose anti-vaccine antibody titer.

    From Day 1 to Day 22

  • Percentage of Participants With Anti-vaccine Antibody Seroprotection Rate (SPR)

    SPR is defined as the percentage of dosed participants with a anti-vaccine antibody titer ≥ 1:40.

    At Day 22

Secondary Outcomes (4)

  • GMT of Anti-vaccine Antibody Titers

    At Day 62 and Day 183

  • GMI of Anti-vaccine Antibody Titers From Day 1 (Baseline) to Day 62

    From Day 1 to Day 62

  • GMI of Anti-vaccine Antibody Titers From Day 1 (Baseline) to Day 183

    From Day 1 to Day 183

  • Percentage of Participants With Anti-vaccine Antibody SPR

    At Day 62 and Day 183

Study Arms (13)

Flu mRNA_Dose level 1_Younger adults (YA)

EXPERIMENTAL

On Day 1, YA adult participants received Flu mRNA at Dose Level 1, the lowest concentration evaluated in the study.

Biological: GSK4382276A Dose level 1

Flu mRNA_Dose level 2 _YA

EXPERIMENTAL

On Day 1, YA participants received Flu mRNA at Dose Level 2, which corresponds to a higher concentration than Dose Level 1.

Biological: GSK4382276A Dose level 2

Flu mRNA_Dose level 3_YA

EXPERIMENTAL

On Day 1, YA participants received Flu mRNA at Dose Level 3, which corresponds to a higher concentration than Dose Level 2.

Biological: GSK4382276A Dose level 3

Flu mRNA_Dose level 4_YA

EXPERIMENTAL

On Day 1, YA participants received Flu mRNA at Dose Level 4, which corresponds to a higher concentration than Dose Level 3.

Biological: GSK4382276A Dose level 4

Flu mRNA_Dose level 5_YA

EXPERIMENTAL

On Day 1, YA participants received Flu mRNA at Dose Level 5, which corresponds to a higher concentration than Dose Level 4.

Biological: GSK4382276A Dose level 5

Flu mRNA_Dose level 6_YA

EXPERIMENTAL

On Day 1, YA participants received Flu mRNA at Dose Level 6, which corresponds to a higher concentration than Dose Level 5.

Biological: GSK4382276A Dose level 6

Flu mRNA_Dose level 7_YA

EXPERIMENTAL

On Day 1, YA participants received Flu mRNA at Dose Level 7, which corresponds to a higher concentration than Dose Level 6.

Biological: GSK4382276A Dose level 7

Flu mRNA_ Dose level 8_YA

EXPERIMENTAL

On Day 1, YA participants received Flu mRNA at Dose Level 8, which corresponds to a higher concentration than Dose Level 7.

Biological: GSK4382276A Dose level 8

Flu mRNA_Dose level 9_YA

EXPERIMENTAL

On Day 1, YA participants received Flu mRNA at Dose Level 9, which corresponds to a higher concentration than Dose Level 8.

Biological: GSK4382276A Dose level 9

Flu mRNA_Dose level 10_YA

EXPERIMENTAL

On Day 1, YA participants received Flu mRNA at Dose Level 10, the highest concentration evaluated in the study.

Biological: GSK4382276A Dose level 10

Pooled Control_YA

ACTIVE COMPARATOR

On Day 1, YA participants received a single dose of FDQ21A - NH or FDQ22A -NH administered as a control and were analyzed together as pooled group throughout the study.

Combination Product: FDQ21A-NHCombination Product: FDQ22A-NH

Flu mRNA_Dose level 7_Older adults (OA)

EXPERIMENTAL

On Day 1, OA participants received Flu mRNA at Dose Level 7.

Biological: GSK4382276A Dose level 7

Control_OA

ACTIVE COMPARATOR

On Day 1, OA participants received a single dose of FDQ21A-NH administered at as a control.

Combination Product: FDQ21A-NH

Interventions

Single dose of intervention administered at Day 1

Also known as: Flu mRNA
Flu mRNA_Dose level 4_YA

Single dose of intervention administered at Day 1

Also known as: Flu mRNA
Flu mRNA_Dose level 6_YA

Single dose of intervention administered at Day 1

Also known as: Flu mRNA
Flu mRNA_Dose level 7_Older adults (OA)Flu mRNA_Dose level 7_YA

Single dose of intervention administered at Day 1

Also known as: Flu mRNA
Flu mRNA_ Dose level 8_YA

Single dose of intervention administered at Day 1

Also known as: Flu mRNA
Flu mRNA_Dose level 9_YA

Single dose of intervention administered at Day 1

Also known as: Flu mRNA
Flu mRNA_Dose level 10_YA
FDQ22A-NHCOMBINATION_PRODUCT

Single dose of intervention administered at Day 1

Pooled Control_YA

Single dose of intervention administered at Day 1

Also known as: Flu mRNA
Flu mRNA_Dose level 1_Younger adults (YA)

Single dose of intervention administered at Day 1

Also known as: Flu mRNA
Flu mRNA_Dose level 2 _YA

Single dose of intervention administered at Day 1

Also known as: Flu mRNA
Flu mRNA_Dose level 3_YA
FDQ21A-NHCOMBINATION_PRODUCT

Single dose of intervention administered at Day 1

Control_OAPooled Control_YA

Single dose of intervention administered at Day 1

Also known as: Flu mRNA
Flu mRNA_Dose level 5_YA

Eligibility Criteria

Age18 Years - 80 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64), Older Adult (65+)

You may qualify if:

  • A male or female between and including 18 and 45 years of age (YAs) or between and including 60 and 80 years of age (OAs) at the time of the study intervention administration. The age of sentinel participants in OA category will be limited to maximum 70 years.
  • Healthy or medically stable participants as established by medical history, safety laboratory assessments and clinical examination.
  • Body mass index \>= 18 kg/m\^2 and \<= 32 kg/m\^2.
  • Participants who, in the opinion of the investigator, can and will comply with the requirements of the protocol.
  • Written informed consent obtained from the participant prior to performing any study-specific procedure.
  • Female participants of non-childbearing potential may be enrolled in the study.
  • Female participants of childbearing potential may be enrolled in the study if the participant:
  • has practiced adequate contraception for 28 days prior to study intervention administration, and
  • has a negative pregnancy test on the day of study intervention administration, and
  • has agreed to continue adequate contraception for at least 1 month after study intervention administration.

You may not qualify if:

  • Medical conditions
  • Acute or chronic clinically significant pulmonary, cardiovascular, hepatic or renal functional abnormality, as determined by physical examination or review of the participant's medical record.
  • Any clinically significant hematological coagulation or urine analysis laboratory abnormality.
  • \* The investigator should use his/her clinical judgement to decide which abnormalities are clinically significant.
  • Current or past malignancy, unless completely resolved without sequelae for \>5 years.
  • Any confirmed or suspected immunosuppressive or immunodeficient condition, including HIV infection, based on medical history and physical examination (no laboratory testing required).
  • History of any reaction or hypersensitivity likely to be exacerbated by any component of the study intervention (including latex, poly-ethylene-glycol, egg protein and aminoglycoside antibiotics).
  • Recurrent history or uncontrolled neurological disorders or seizures, including Guillain-BarrĂ© syndrome and Bell's palsy, with the exception of febrile seizures during childhood.
  • Any medical condition that in the judgment of the investigator would make intramuscular injection unsafe.
  • Any other clinical condition that, in the opinion of the investigator, might pose additional risk to the participant due to participation in the study.
  • Significant exposure to persons with influenza or laboratory-confirmed SARS-CoV-2 within 7 days prior to Visit 1 (Day 1) and for whom a SARS-CoV-2 PCR test has not (yet) been confirmed as negative.
  • Prior/Concomitant therapy
  • Administration of seasonal influenza vaccine within 180 days before enrollment or planned administration up to Visit 4 (Day 29).
  • Administration of a vaccine not foreseen by the study protocol in the period starting 28 days before the study intervention administration, or planned administration within 28 days after the study intervention administration\*, with the exception of vaccines authorized or approved for the prevention of COVID-19 (regardless of the type of vaccine).
  • \*In case emergency mass vaccination for an unforeseen public health threat is organized by public health authorities outside the routine immunization program, the time period described above can be reduced to 7 days, if necessary, for that mass vaccination vaccine, provided it is used according to the local governmental recommendations and that the Sponsor is notified accordingly.
  • +11 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (7)

GSK Investigational Site

Edegem, 2650, Belgium

Location

GSK Investigational Site

Ghent, 9000, Belgium

Location

GSK Investigational Site

Halifax, Nova Scotia, B3J 3G9, Canada

Location

GSK Investigational Site

Sherbrooke, Quebec, J1L 0H8, Canada

Location

GSK Investigational Site

Madrid, 28006, Spain

Location

GSK Investigational Site

Madrid, 28046, Spain

Location

GSK Investigational Site

Madrid, 28222, Spain

Location

Related Publications (1)

  • Leroux-Roels I, De Coster I, Borobia AM, Langley JM, Ochoa Mazarro D, Ruiz-Antoran B, Berre S, Germain S, Knauer C, Koch SD, Mann P, Mesia Vela D, Ovbude LJ, Pardo IA, Srivastava B, Moldt B, Nordgren M. Safety and immunogenicity of an investigational mRNA-lipid nanoparticle-based monovalent influenza vaccine: Results from a phase 1, randomized, dose-escalation study. Hum Vaccin Immunother. 2026 Dec;22(1):2631804. doi: 10.1080/21645515.2026.2631804. Epub 2026 Feb 17.

MeSH Terms

Conditions

Influenza, Human

Condition Hierarchy (Ancestors)

Respiratory Tract InfectionsInfectionsOrthomyxoviridae InfectionsRNA Virus InfectionsVirus DiseasesRespiratory Tract Diseases

Results Point of Contact

Title
GSK Response Center
Organization
GlaxoSmithKline

Study Officials

  • GSK Clinical Trials

    GlaxoSmithKline

    STUDY DIRECTOR

Publication Agreements

PI is Sponsor Employee
No
Restriction Type
OTHER
Restrictive Agreement
Yes

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
QUADRUPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
Masking Details
Data were collected in an observer-blind manner: participants and site evaluators were blinded, while the sponsor and certain study personnel were aware of treatment assignments. For Dose Levels 1, 2, 5, and 6, data were collected in a single-blind manner-investigators were aware of the intervention, but participants were blinded.
Purpose
PREVENTION
Intervention Model
SEQUENTIAL
Sponsor Type
INDUSTRY
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 28, 2022

First Posted

July 7, 2022

Study Start

August 9, 2022

Primary Completion

March 26, 2024

Study Completion

March 26, 2024

Last Updated

March 10, 2026

Results First Posted

March 10, 2026

Record last verified: 2026-02

Data Sharing

IPD Sharing
Will share

IPD for this study will be made available via the Clinical Study Data Request site.

Shared Documents
STUDY PROTOCOL, SAP, ICF, CSR
Time Frame
IPD will be made available within 6 months of publishing the results of the primary endpoints, a key secondary endpoints and safety data of the study.
Access Criteria
Access is provided after a research proposal is submitted and has received approval from the Independent Review Panel and after a Data Sharing Agreement is in place. Access is provided for an initial period of 12 months but an extension can be granted, when justified, for up to another 12 months.

Locations