Combined Effect of Exercise Intensity With Nutritional Supplementation on Skeletal Muscle Function

NCT05350566 | Completed DMC

• 1 other identifier: 2021-00373
• Study Type: interventional
• Target: 25
• Locations: 1 country
• Sites: 1

Brief Summary

The main objective of this study is to determine the effectiveness of a nutritional intervention (olive leaf extract) combined with a specific training session (MICT or SIT) on PDH activation (as well as other protein expressions) and exercise-induced muscle fatigue.

Conditions

Healthy Volunteers; Exercise Intensity; Neuromuscular Fatigue; Skeletal Muscle Adaptations

Outcome Measures

Primary Outcomes (3)

• PDH dephosphorylation

  protein involved in skeletal muscle metabolism

  before, immediately after and 24 hours after exercise completion

• expression of OXPHOS proteins

  proteins used as markers of oxidative phosphorylation complexes

  before, immediately after and 24 hours after exercise completion

• expression of MCU protein

  protein involved in the transport of Ca2+ in the mitochondria

  before, immediately after and 24 hours after exercise completion

Secondary Outcomes (4)

• Maximal voluntary contraction force

  before, immediately after and 24 hours after exercise completion

• Voluntary activation level

  before, immediately after and 24 hours after exercise completion

• Peak doublet evoked at 100 Hz

  before, immediately after and 24 hours after exercise completion

• M-wave amplitude

  before, immediately after and 24 hours after exercise completion

Study Arms (2)

Sprint interval training group (SIT group)

EXPERIMENTAL

The model of sprint interval training (SIT) consists of six 30 s all-out cycling bouts on a cycle ergometer, with 4 min rests between tests.

Dietary Supplement: Olive leaf extract; Dietary Supplement: Placebo

Moderate intensity continuous training (MICT group)

EXPERIMENTAL

The moderate intensity continuous training (MICT) consists of 1h cycling on a cycle ergometer at 50% of the maximal aerobic power output

Dietary Supplement: Olive leaf extract; Dietary Supplement: Placebo

Interventions

Olive leaf extract DIETARY_SUPPLEMENT

A capsule containing 250 mg of an olive leaf extract preparation will be swallowed with a glass of water before exercise.

Moderate intensity continuous training (MICT group); Sprint interval training group (SIT group)

Placebo DIETARY_SUPPLEMENT

A capsule containing 336 mg of cellulose microcrystalline will be swallowed with a glass of water before exercise.

Moderate intensity continuous training (MICT group); Sprint interval training group (SIT group)

Eligibility Criteria

• Age: 18 Years - 40 Years
• Sex: male
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Being male (including females would necessitate increasing the number of included participants to consider sex as a variable which would burden the project too much).
• Being aged between 18 and 40 years
• Being healthy (The assessment will be done through a questionnaire and an interview/examination by a medical doctor if there is any doubts)
• Being physically active, but not involved in any structured training program
• Having stable dietary habits

You may not qualify if:

• Having previous medical events that would put the participant at risk during the study
• Ingesting caffeine on the experimental days
• Not being currently treated for blood clotting problems
• Taking aspirin or anti-coagulant drugs in the 48 hours preceding the experimental days in which muscle samples will be collected
• Having allergy to xylocaine
• Having food allergy

Sponsors & Collaborators

• University of Lausanne: lead
• Société des Produits Nestlé (SPN): collaborator

Study Sites (1)

Bâtiment Synathlon, quartier UNIL-Centre

Lausanne, 1015, Switzerland

Location

Related Publications (2)

• Place N, Ivarsson N, Venckunas T, Neyroud D, Brazaitis M, Cheng AJ, Ochala J, Kamandulis S, Girard S, Volungevicius G, Pauzas H, Mekideche A, Kayser B, Martinez-Redondo V, Ruas JL, Bruton J, Truffert A, Lanner JT, Skurvydas A, Westerblad H. Ryanodine receptor fragmentation and sarcoplasmic reticulum Ca2+ leak after one session of high-intensity interval exercise. Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):15492-7. doi: 10.1073/pnas.1507176112. Epub 2015 Nov 2.

  PMID: 26575622 BACKGROUND

• Zanou N, Dridi H, Reiken S, Imamura de Lima T, Donnelly C, De Marchi U, Ferrini M, Vidal J, Sittenfeld L, Feige JN, Garcia-Roves PM, Lopez-Mejia IC, Marks AR, Auwerx J, Kayser B, Place N. Acute RyR1 Ca2+ leak enhances NADH-linked mitochondrial respiratory capacity. Nat Commun. 2021 Dec 10;12(1):7219. doi: 10.1038/s41467-021-27422-1.

  PMID: 34893614 BACKGROUND

MeSH Terms

Interventions

olive leaf extract

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: QUADRUPLE
• Who Masked: PARTICIPANT, CARE PROVIDER, INVESTIGATOR, OUTCOMES ASSESSOR
• Masking Details: One box containing the product and one containing the placebo will be labeled A or B. The code will be kept in a sealed envelope which will be revealed only at the end of the study or in case of an adverse event.
• Purpose: BASIC SCIENCE
• Intervention Model: CROSSOVER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Principal Investigator

Model Details: The design is a placebo-controlled, double blind, cross-over two-arm study

Study Record Dates

• First Submitted: April 14, 2022
• First Posted: April 28, 2022
• Study Start: June 7, 2022
• Primary Completion: December 7, 2022
• Study Completion: December 7, 2022
• Last Updated: May 10, 2023

Record last verified: 2023-05

Locations

CH (1)