NCT05308342

Brief Summary

This study was a single-center, randomized, controlled prospective study. Those who had premature ovarian failure and who had fertility requirements were enrolled in the study. To determine the efficacy and safety of umbilical cord mesenchymal stem cells in the treatment of patients with POI.

Trial Health

43
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
66

participants targeted

Target at P50-P75 for not_applicable

Timeline
Completed

Started Nov 2019

Longer than P75 for not_applicable

Geographic Reach
1 country

1 active site

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

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Study Timeline

Key milestones and dates

Study Start

First participant enrolled

November 20, 2019

Completed
5 days until next milestone

First Submitted

Initial submission to the registry

November 25, 2019

Completed
2.4 years until next milestone

First Posted

Study publicly available on registry

April 4, 2022

Completed
1.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 31, 2023

Completed
4 months until next milestone

Study Completion

Last participant's last visit for all outcomes

December 1, 2023

Completed
Last Updated

April 4, 2022

Status Verified

March 1, 2022

Enrollment Period

3.7 years

First QC Date

November 25, 2019

Last Update Submit

March 25, 2022

Conditions

Outcome Measures

Primary Outcomes (1)

  • Follicular development rate

    Follicular development and hormonal examination were performed 1 week before treatment and at 1, 2, and 3 weeks after treatment to evaluate the development of follicles. If there is no follicular development in the third week, stop the drug after 1 week, and contact the next treatment time after menstruation. During the follow-up period to half a year after the end of the last treatment, the follicular development activity rate was observed.

    9-12 months

Secondary Outcomes (2)

  • Changes in blood flow index in the ovary

    9-12 months

  • Clinical pregnancy rate

    9-12 months

Study Arms (2)

UC-MSCs+hormone replacement group

EXPERIMENTAL

Group A was the hormone replacement combined with transplantation of umbilical cord mesenchymal stem cells group(test group).

Procedure: transplantation of human UC-MSCs into ovaries of POI patientsDrug: hormone replacement treatment

hormone replacement group

ACTIVE COMPARATOR

Group B was the hormone replacement group (control group).

Drug: hormone replacement treatment

Interventions

UC-MSCs (GMP grade, from Clinical Center for Stem Cell Research of the Affiliated Drum Tower Hospital of Nanjing University Medical School, licensed by the China Food and Drug Administration) are injected into the ovary of patients under transvaginal ultrasonographic (TVUS)-guidance. A total number of 10×106 cells, 5×106 for unilateral ovarian injection) is immediately preserved and transferred for direct injection. After vaginal sterilization, TVUS-guided transplantation is performed by two senior-level medical physicians, using a SIEMENS ACUSON ANTANES premium edition system (SIEMENS AG Healthcare Sector, Erlangen, Germany), equipped with a 6-10 MHz probe. The UC-MSC solution is injected into the ovary by using 21-G PTC needles (Hakko Medical Co, Japan) under TVUS guidance.

UC-MSCs+hormone replacement group

Routine estrogen progesterone replacement periodic therapy

Also known as: HRT
UC-MSCs+hormone replacement grouphormone replacement group

Eligibility Criteria

Age20 Years - 39 Years
Sexfemale
Healthy VolunteersNo
Age GroupsAdult (18-64)

You may qualify if:

  • Those who meet the POF diagnostic criteria and have no spontaneous follicular activity;
  • Married, 20 years old ≤ age \< 40 years old;
  • The average diameter of each ovary is \> 10 mm;
  • Have agreed to sign the informed consent form.

You may not qualify if:

  • Female and/or male chromosomal abnormalities;
  • Endometriosis, adenomyosis;
  • Postoperative ovarian borderline or malignant tumor;
  • Uterine dysplasia;
  • Associated with female autoimmune disease or other serious internal surgical diseases;
  • hormone replacement contraindications;
  • In the past 1 year, had received an experimental study of premature ovarian failure in the external hospital;
  • Male azoospermia or severe oligozoospermia.

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (1)

Reproductive Medicine Center, The affiliated Drum Towel Hospital of Nanjing University Medical School

Nanjing, Jiangsu, 210008, China

RECRUITING

MeSH Terms

Conditions

Primary Ovarian Insufficiency

Condition Hierarchy (Ancestors)

Ovarian DiseasesAdnexal DiseasesGenital Diseases, FemaleFemale Urogenital DiseasesFemale Urogenital Diseases and Pregnancy ComplicationsUrogenital DiseasesGenital DiseasesGonadal DisordersEndocrine System Diseases

Study Officials

  • Guangshu Han, phD

    The Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Haixiang Sun, phD

CONTACT

Lijun Ding, phD

CONTACT

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR
Purpose
TREATMENT
Intervention Model
PARALLEL
Sponsor Type
OTHER
Responsible Party
SPONSOR INVESTIGATOR
PI Title
principal investigator

Study Record Dates

First Submitted

November 25, 2019

First Posted

April 4, 2022

Study Start

November 20, 2019

Primary Completion

July 31, 2023

Study Completion

December 1, 2023

Last Updated

April 4, 2022

Record last verified: 2022-03

Locations