Impact of Muscle Temperature on Muscle Growth and Breakdown: Cooling During Resistance Training

NCT05302791 | Completed Results FDA Device

• 1 other identifier: 0072-22-FB
• Study Type: interventional
• Target: 12
• Locations: 1 country
• Sites: 1

Brief Summary

The purpose of this study is to examine the effect of human skeletal muscle temperature during resistance exercise on myogenic and proteolytic signaling. Subjects will perform bilateral resistance exercise bouts at an intensity that should stimulate a muscle growth response in the Vastus Lateralis. During the resistance exercise, the subjects will receive a cold (10°C) intervention on the experimental limb, while receiving a neutral temperature intervention (22°C) on the control limb.

Conditions

Exercise Training

Keywords

Resistance Exercise; Muscle Cooling

Outcome Measures

Primary Outcomes (10)

• RT-qPCR for Forkhead Box O3 (FOXO3a)

  RT-qPCR for Forkhead Box O3 (FOXO3a) \[mRNA response FOXO3a\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.

  4-hours post Exercise

• RT-qPCR for Tripartite Motif Containing 63 (TRIM63)

  RT-qPCR for Tripartite Motif Containing 63 (TRIM63) \[mRNA response of TRIM63\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.

  4-hours post Exercise

• RT-qPCR for Growth/Differentiation Factor 8 (MSTN)

  RT-qPCR for Growth/Differentiation Factor 8 (MSTN) \[mRNA response of MSTN\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.

  4-hours post Exercise

• RT-qPCR for Myogenic Factor 5 (MYF5)

  RT-qPCR for Myogenic Factor 5 (MYF5) \[mRNA response of MYF5\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.

  4-hours post Exercise

• RT-qPCR for Myogenic Factor 6 (MYF6)

  RT-qPCR for Myogenic Factor 6 (MYF6) \[mRNA response of MYF6\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.

  4-hours post Exercise

• RT-qPCR for Myocyte Enhancer Factor 2A (MEF2a)

  RT-qPCR for Myocyte Enhancer Factor 2A (MEF2a) \[mRNA response of MEF2a\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.

  4-hours post Exercise

• RT-qPCR for Myogenic Differentiation 1 (MYOD1)

  RT-qPCR for Myogenic Differentiation 1 (MYOD1) \[mRNA response of MYOD1\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.

  4-hours post Exercise

• RT-qPCR for Myogenin (MYOG)

  RT-qPCR for Myogenin (MYOG) \[mRNA response of MYOG\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.

  4-hours post Exercise

• RT-qPCR for Ribosomal Protein S3 (RPS3)

  RT-qPCR for Ribosomal Protein S3 (RPS3) \[mRNA response of RPS3\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.

  4-hours post Exercise

• RT-qPCR for Ribosomal Protein L3 Like (RPL3L)

  RT-qPCR for Ribosomal Protein L3 Like (RPL3L) \[mRNA response of RPL3L\] Fold change calculated using the delta, delta CT method relative to stable reference genes and pre-exercise measures.

  4-hours post Exercise

Secondary Outcomes (2)

• Muscle Temperature

  4-hours post Exercise

• Skin Temperature

  4-hours post Exercise

Study Arms (2)

Muscle Cooling

EXPERIMENTAL

The intervention will be applying a cooling wrap during the bilateral resistance exercise.

Device: Muscle Cooling; Other: Resistance Exercise

Control

PLACEBO COMPARATOR

There will be no temperature alteration for this leg during bilateral resistance exercise.

Other: Resistance Exercise

Interventions

Muscle Cooling DEVICE

Cooling wrap will be set to 10°C

Muscle Cooling

Resistance Exercise OTHER

Resistance Machines (Leg Press and Knee extension). 12 rep Max

Control; Muscle Cooling

Eligibility Criteria

• Age: 19 Years - 45 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)

You may qualify if:

• Any male or female who is 19-45 of age and is considered
• low risk for physical activity based on the American College of Sports Medicine (ACSM) guidelines
• recreationally active during the 6 weeks prior to participation by meeting the ACSM minimum activity guidelines

You may not qualify if:

• Any person outside of the age range of 19-45
• not considered 'low risk' on the ACSM Risk Stratification Form
• not meeting the exercise requirements for the preceding 6 weeks will be excluded.
• self-report of a history of joint or muscular injury that would prevent them from safely completing the required activities
• self-report of taking prescription drugs or supplements that may interfere with this research
• self-report allergy to Xylocaine
• pregnant
• breast feeding
• self-report sensitivity to heat or cold

Sponsors & Collaborators

• University of Nebraska: lead

Study Sites (1)

University of Nebraska at Omaha

Omaha, Nebraska, 68182, United States

Location

MeSH Terms

Interventions

Resistance Training

Intervention Hierarchy (Ancestors)

Exercise Therapy; Rehabilitation; Aftercare; Continuity of Patient Care; Patient Care; Therapeutics; Physical Therapy Modalities; Physical Conditioning, Human; Exercise; Motor Activity; Movement; Musculoskeletal Physiological Phenomena; Musculoskeletal and Neural Physiological Phenomena

Results Point of Contact

• Title: Dustin Slivka
• Organization: University of Montana

dustin.slivka@umontana.edu

406-243-2389

Study Officials

• Dustin R Slivka, PHD

  University of Nebraska

  PRINCIPAL INVESTIGATOR

Publication Agreements

• PI is Sponsor Employee: No
• Restrictive Agreement: No

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: RANDOMIZED
• Masking: NONE
• Purpose: OTHER
• Intervention Model: PARALLEL
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Model Details: Each Subject acts as their own control in a de facto repeated measures design, where there is a Single bilateral exercise bout, with experimental and control assignment to individual limbs.

Study Record Dates

• First Submitted: March 7, 2022
• First Posted: March 31, 2022
• Study Start: April 19, 2022
• Primary Completion: October 1, 2022
• Study Completion: October 1, 2022
• Last Updated: October 8, 2024
• Results First Posted: April 24, 2024

Record last verified: 2024-10

Data Sharing

• IPD Sharing: Will not share

Locations

US (1)