The Effect of an Acute Bout of Exercise on Serum Vitamin D Concentration

NCT05214027 | Completed

• 1 other identifier: EP 19/20 026
• Study Type: observational
• Target: 34
• Locations: 1 country
• Sites: 1

Brief Summary

Vitamin D deficiency is considered a public health priority in the UK, with approximately 30-40% of the UK population being deemed vitamin D deficient during winter months. Current government strategies to improve vitamin D status amongst the UK population involve dietary supplementation, however, it has been shown that excess adiposity reduces the impact of dietary supplementation with vitamin D. One potential explanation for this observation is that vitamin D becomes sequestered in adipose tissue. We hypothesise that exercise may facilitate the mobilisation of vitamin D from adipose tissue and thus increase circulating vitamin D (25OHD) concentrations. Little is currently known as to whether a single bout of exercise affects vitamin D status, with a handful of studies demonstrating contradictory findings. This research will examine the effect of an acute bout of exercise (treadmill-based at 60% VO2 Max for 60 minutes) on vitamin D status (serum 25(OH)D) in healthy community-dwelling adults.

Conditions

Vitamin D; Acute Exercise

Keywords

Vitamin D; Acute Exercise; Adults; United Kingdom; Lipid Mobilisation

Outcome Measures

Primary Outcomes (1)

• Serum vitamin D concentration

  As assessed by serum 25(OH)D via LC-MS

  24 hours- change from baseline to 24 hours post exercise

Secondary Outcomes (3)

• Markers of lipid mobilisation

  24 hours- change from baseline to 24 hours post exercise

• Calcium

  24 hours- change from baseline to 24 hours post exercise

• Other vitamin D metabolites

  24 hours- change from baseline to 24 hours post exercise

Study Arms (1)

Participants

This study follows a randomised crossover design. All participants will undergo a single 60-minute treadmill-based exercise intervention, and a resting period for equal duration in a randomised order.

Other: Treadmill-Based Exercise (60% VO2 Max for 60 minutes); Other: Rest (60 minutes)

Interventions

Treadmill-Based Exercise (60% VO2 Max for 60 minutes) OTHER

Each participant will undergo a single 60-minute treadmill-based exercise intervention in a randomised order. Treadmill settings are generated based on settings which correspond to participants exercising at 60% VO2 max from their maximal exercise test on visit 2. To confirm that participants are exercising at 60% during the hour session, 1 minute samples of expired air are taken at 15 minute intervals and immediately analysed to check % VO2 max. Participants heart rate and RPE measures are also taken at 15 minute intervals throughout the exercise session and cross-checked against predicted values at 60% VO2 Max.

Participants

Rest (60 minutes) OTHER

Each participant will undergo a 60-minute rest period in a randomised order.

Participants

Eligibility Criteria

• Age: 25 Years - 65 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Non-Probability Sample

Study Population

Healthy, community-dwelling adults (aged 25-65 years old)

You may not qualify if:

• years old
• Ability to travel to and from University of Bath campus
• Current or recent use (within the last three months) of weight loss drugs, medication that would interfere with study outcomes, sunbeds, or dietary vitamin D supplementation
• Change in weight of more than 5% in the last six months, or a significant change in lifestyle in the last two months
• Positive responses to the Physical Activity Readiness Questionnaire (PAR-Q)
• Presence of coronary heart disease, chronic kidney disease, type 2 diabetes, stroke, heart failure, peripheral arterial disease, severe hypertension (blood pressure \>180/110 mmHg)
• BMI \<18kg/m2 or BMI \>40kg/m2

Sponsors & Collaborators

• University of Bath: lead
• Biotechnology and Biological Sciences Research Council: collaborator

Study Sites (1)

Sophie Ella Davies

Bath, Somerset, BA1 6AW, United Kingdom

Location

Biospecimen

Retention: SAMPLES WITHOUT DNA

Human Plasma and Serum

MeSH Terms

Interventions

RE1-silencing transcription factor

Study Design

• Study Type: observational
• Observational Model: OTHER
• Time Perspective: OTHER
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: PhD Student

Study Record Dates

• First Submitted: January 12, 2022
• First Posted: January 28, 2022
• Study Start: April 1, 2021
• Primary Completion: November 1, 2021
• Study Completion: November 1, 2021
• Last Updated: November 14, 2022

Record last verified: 2022-11

Data Sharing

• IPD Sharing: Will not share

Locations

GB (1)