To Exam the Effects of Phyllanthus Niruri Extracts on Human Neutrophils

NCT05206058 | Completed

• 1 other identifier: 202002189B0
• Study Type: observational
• Target: 240
• Locations: 1 country
• Sites: 1

Brief Summary

To exam the effects of Phyllanthus niruri extracts(corilagin, phyllanthin and brevifolin) on human neutrophils

Conditions

Neutrophil; Phyllanthus Abnormis Poisoning

Outcome Measures

Primary Outcomes (2)

• Measurement of superoxide anion release

  Superoxide anion released from human neutrophils were determined by measuring ferricytochrome c reduction.The neutrophils were activated with fMLF (30 nM) and cytochalasin B(0.5 μg/mL) (fMLF/CB). Changes in absorbance that occurred at 550 nm were observed continuously using a double beam spectrophotometer. Superoxide anion level was calculated using the methods described in previous report.

  After neutrophil isolation, an average of 3 months

• Measurement of intracellular ROS formation

  Neutrophil ROS production was determined from the conversion of non-fluorescent DHR 123 to fluorescent rhodamine 123, detected using flow cytometry. Neutrophils (2 × 106 cells/ml) were incubated with DHR 123 (2 μM) for 15 min at 37°C, and then treated with honokiol (0.1-10 μM) for 5 min before the addition of fMLP/CB (0.5 μg/ml) for a further 5 min. The change in fluorescence was analysed using flow cytometry

  After neutrophil isolation, an average of 3 months

Interventions

venipuncture PROCEDURE

Plan to enroll 240 health people who are 20-35 years of age. People with received veinpuncture. Blood will be collected at time and drawn into Heparin-coated tube.

Eligibility Criteria

• Age: 20 Years - 35 Years
• Sex: all
• Healthy Volunteers: Yes
• Age Groups: Adult (18-64)
• Sampling Method: Non-Probability Sample

Study Population

Voluntary healthy donor (20-35 years old) do not have coagulopathy, systemic infective disease and severe liver and renal function impairment.

You may qualify if:

• Healthy volunteers who aged 20-35 years.
• Do not have coagulopathy, systemic infection disease and severe liver and renal function impairment
• Accept venipuncture

You may not qualify if:

• Healthy volunteers who aged over 35 years.
• Have coagulopathy, systemic infection disease and severe liver and renal function impairment

Sponsors & Collaborators

• Chang Gung Memorial Hospital: lead

Study Sites (1)

Chang Gung Memorial Hospital

Taoyuan District, 333, Taiwan

Location

Biospecimen

Retention: SAMPLES WITH DNA

Seperation of human neutrophils were according to a standardized procedures and following steps: using 3%(w/v) dextran sedimentation for 30 min, then gradient centrifugation in Ficoll-Hypaque, and hypotonic lysis of erythrocytes. The purified neutrophils were determined by the Trypan blue exclusion method and need to contained \>98% viable cells. Then they were suspended in without calcium ion HBSS buffer (pH 7.4), and stored at 4 °C for analysis

MeSH Terms

Interventions

Phlebotomy

Intervention Hierarchy (Ancestors)

Blood Specimen Collection; Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Therapeutics; Surgical Procedures, Operative; Investigative Techniques

Study Design

• Study Type: observational
• Observational Model: COHORT
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: SPONSOR

Study Record Dates

• First Submitted: January 23, 2022
• First Posted: January 25, 2022
• Study Start: January 1, 2022
• Primary Completion: September 30, 2022
• Study Completion: July 31, 2023
• Last Updated: September 1, 2023

Record last verified: 2022-01

Locations

TW (1)