NCT05155579

Brief Summary

This is a Phase Ib trial conducted in Bougouni, Mali to evaluate the safety and immunogenicity of R21/Matrix-M in a single and two vial presentation, with different immunisation schedules, and when co-administered with EPI vaccines in African children.

Trial Health

90
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
594

participants targeted

Target at P75+ for phase_1

Timeline
Completed

Started May 2022

Typical duration for phase_1

Geographic Reach
2 countries

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

December 9, 2021

Completed
4 days until next milestone

First Posted

Study publicly available on registry

December 13, 2021

Completed
5 months until next milestone

Study Start

First participant enrolled

May 14, 2022

Completed
3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 27, 2025

Completed
Same day until next milestone

Study Completion

Last participant's last visit for all outcomes

May 27, 2025

Completed
Last Updated

April 8, 2026

Status Verified

September 1, 2025

Enrollment Period

3 years

First QC Date

December 9, 2021

Last Update Submit

April 7, 2026

Conditions

Outcome Measures

Primary Outcomes (2)

  • Safety

    Solicited adverse events: * Occurrence of solicited local reactogenicity signs and symptoms for 7 days following the vaccination. * Occurrence of solicited systemic reactogenicity signs and symptoms for 7 days following the vaccination. Unsolicited adverse events * Occurrence of unsolicited adverse events for 28 days following the vaccination. Laboratory adverse events * Change from baseline for safety laboratory measures thought to be clinically significant. Serious adverse events • Occurrence of serious adverse events for the whole study duration.

    2 years

  • Immunogenicity

    * To assess the humoral immunogenicity of R21/Matrix-M as a single- vial formulation in 5-36-month-old African children, compared with the two-vial formulation, 0, 30, 180 and 365 days after the administration of the third dose of R21/Matrix-M; and 0, 30, 180 and 365 days after the administration of a booster dose. * To assess the humoral immunogenicity of EPI vaccines given at 9 months, measles-rubella and yellow fever vaccines, when given with and without R21/Matrix-M, 0, 30, 180 and 360 days after the administration of the third dose of R21/Matrix-M * To assess the humoral immunogenicity of EPI vaccines given at 6, 10 and 14 weeks of age, pentavalent and oral polio vaccines, given as part of EPI at 6, 10 and 14 weeks of age, when given with and without R21/Matrix-M, 0, 30, 180 and 360 days after the administration of the third dose of R21/Matrix-M or the EPI vaccines.

    2 years

Secondary Outcomes (2)

  • Safety of a delayed third dose

    2 years

  • Immunogenicity of a delayed third dose

    2 years

Study Arms (9)

Groups 1a, 2a and 3a

EXPERIMENTAL

60 children, aged 5-36 months, who will receive 4 doses of 5µg R21/50µg Matrix-M as a two vial formulation. The first three doses will be given one month apart, followed by a booster vaccination 12 months after the third dose. The age range of 5-36 months has been split into three groups to ensure an even age spread across age groups. Group 1a is 20 children aged 5-11 months, group 2a is 20 children aged 12-23 months, and group 3a is 20 children aged 24-36 months.

Biological: R21/Matrix-M - two vial formulation

Group 1b, 2b and 3b

EXPERIMENTAL

60 children, aged 5-36 months, who will receive 4 doses of 5µg R21/50µg Matrix-M as a single vial formulation. The first three doses will be given one month apart, followed by a booster vaccination 12 months after the third dose. The age range of 5-36 months has been split into three groups to ensure an even age spread across age groups. Group 1b is 20 children aged 5-11 months, group 2b is 20 children aged 12-23 months, and group 3b is 20 children aged 24-36 months.

Biological: R21/Matrix-M - single vial formulation

Group 4a

EXPERIMENTAL

150 participants, aged 6-7 months at the time of randomisation (to ensure third vaccination is given at approximately 9 months), who will receive 3 doses of 5µg R21/50µg Matrix-M one month apart. At the time of the third dose they will receive their measles-rubella and yellow fever vaccinations at the same time as R21/Matrix-M.

Biological: R21/Matrix-M - single vial formulationBiological: Licensed vaccine - Measles-rubellaBiological: Licensed vaccine - Yellow fever

Group 4b

ACTIVE COMPARATOR

150 participants, aged 6-7 months at the time of randomisation, who will receive a measles-rubella and yellow fever vaccination 2 months after randomisation.

Biological: Licensed vaccine - Measles-rubellaBiological: Licensed vaccine - Yellow fever

Group 4c

EXPERIMENTAL

Group 4c is 50 participants, aged 6-7 months at the time of randomisation, who will receive 3 doses of 5µg R21/50µg Matrix-M one month apart.

Biological: R21/Matrix-M - single vial formulation

Group 5a

EXPERIMENTAL

30 children who will receive 3 doses of 5µg R21/50µg Matrix-M, pentavalent, rotavirus, pneumococcal and OPV vaccines at 6, 10 and 14 weeks of age. They will receive IPV two weeks following the third dose.

Biological: R21/Matrix-M - single vial formulationBiological: Licensed vaccine - Pentavalent (diphtheria, tetanus, pertussis, hepatitis B and Hib)Biological: Licensed vaccine - Oral Polio Vaccine (OPV)Biological: Licensed vaccine - RotavirusBiological: Licensed vaccine - Pneumococcal vaccineBiological: Licensed vaccine - Inactivated Polio Vaccine (IPV)

Group 5b

ACTIVE COMPARATOR

30 children who will receive 3 doses of pentavalent, rotavirus, pneumococcal and OPV vaccines at 6, 10 and 14 weeks of age. They will receive IPV two weeks following the third dose.

Biological: Licensed vaccine - Pentavalent (diphtheria, tetanus, pertussis, hepatitis B and Hib)Biological: Licensed vaccine - Oral Polio Vaccine (OPV)Biological: Licensed vaccine - RotavirusBiological: Licensed vaccine - Pneumococcal vaccineBiological: Licensed vaccine - Inactivated Polio Vaccine (IPV)

Group 6a

EXPERIMENTAL

30 children, aged 5-36 months, who will receive 3 doses of 5µg R21/50µg Matrix-M. The first two doses one month apart and the third dose 6 months after the first dose.

Biological: R21/Matrix-M - single vial formulation

Group 6b

EXPERIMENTAL

30 children, aged 5-36 months, who will receive 3 doses of 5µg R21/50µg Matrix-M. The first two doses one month apart and the third dose 12 months after the first dose.

Biological: R21/Matrix-M - single vial formulation

Interventions

Adjuvanted malaria vaccine in a single vial formulation

Group 1b, 2b and 3bGroup 4aGroup 4cGroup 5aGroup 6aGroup 6b

Adjuvanted malaria vaccine in a double vial formulation

Groups 1a, 2a and 3a

Licensed vaccine part of the EPI vaccination schedule

Group 4aGroup 4b

Licensed vaccine part of the EPI vaccination schedule

Group 4aGroup 4b

Licensed vaccine part of the EPI vaccination schedule

Group 5aGroup 5b

Licensed vaccine part of the EPI vaccination schedule

Group 5aGroup 5b

Licensed vaccine part of the EPI vaccination schedule

Group 5aGroup 5b

Licensed vaccine part of the EPI vaccination schedule

Group 5aGroup 5b

Licensed vaccine part of the EPI vaccination schedule

Group 5aGroup 5b

Eligibility Criteria

Age6 Weeks - 36 Months
Sexall
Healthy VolunteersYes
Age GroupsChild (0-17)

You may qualify if:

  • Age:
  • Group 1: The child is 5-11 months of age at the time of randomization (i.e. up to the day before of their first birthday).
  • Group 2: The child is 12-23 months of age at the time of randomization (i.e. up to the day before of their second birthday).
  • Group 3: The child is 24-36 months of age at the time of randomization (i.e. up to the day of their third birthday).
  • Group 4: The child is 6-7 months of age at the time of randomization.
  • Group 5: The child is 6 weeks of age at the time of randomization and have not received any dose of the pentavalent vaccine, pneumococcal vaccine, rotavirus vaccine, IPV and only the first dose of the OPV.
  • Group 6: The child is aged 5-36 months at the time of their first vaccination
  • Signed informed consent/thumb-printed and witnessed informed consent obtained from the parent(s)/guardian(s) of the child to join the trial.
  • The investigator believes that the parents/guardians can and will comply with the requirements of the protocol if the child is enrolled in the study.
  • The child is a permanent resident of the study area and likely to remain a resident for the duration of the trial.

You may not qualify if:

  • The child has previously received a malaria vaccine.
  • The child is enrolled in another malaria intervention trial that could interfere with the results of this study.
  • The child has a history of allergic disease or reactions likely to be exacerbated by any component of the study vaccines.
  • The child has a history of allergic reactions, significant IgE-mediated events or anaphylaxis to previous immunisations.
  • The child has major congenital defects.
  • The child has anaemia associated with clinical signs of symptoms of decompensation, or a haemoglobin of ≤7.4 g/dL.
  • The child has had a blood transfusion within one month of enrolment.
  • The child has been administered immunoglobulins and/or any blood products within the three months preceding the planned administration of the vaccine candidate.
  • The child has malnutrition requiring hospital admission.
  • The child has an acute or chronic, clinically significant pulmonary, cardiovascular, gastrointestinal, endocrine, neurological, skin, hepatic or renal functional abnormality, as determined by medical history, physical examination or laboratory tests.
  • Any confirmed or suspected immunosuppressive or immunodeficient state, including HIV or asplenia.
  • The child has received an investigational drug or vaccine other than the study vaccines within 30 days preceding the first dose of study vaccine, or planned use during the study period.
  • The child is currently participating in another clinical trial if likely to affect data interpretation of this trial
  • The child has any significant disease, disorder or situation which, in the opinion of the Investigator, may either put the participants at risk because of participation in the trial, or may influence the result of the trial, or the participant's ability to participate in the trial.
  • Clinically significant laboratory abnormality as judged by the study clinician
  • +2 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Malaria Research & Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Medicine, Pharmacy and Dentistry, University of Sciences Techniques and Technologies of Bamako

Bamako, Mali

Location

CCVTM, University of Oxford

Oxford, United Kingdom

Location

MeSH Terms

Conditions

Malaria

Interventions

Tetanus ToxoidHepatitis B Vaccines

Condition Hierarchy (Ancestors)

Protozoan InfectionsParasitic DiseasesInfectionsMosquito-Borne DiseasesVector Borne Diseases

Intervention Hierarchy (Ancestors)

ToxoidsVaccinesBiological ProductsComplex MixturesViral Hepatitis VaccinesViral Vaccines

Study Officials

  • Adrian Hill

    University of Oxford

    PRINCIPAL INVESTIGATOR

Study Design

Study Type
interventional
Phase
phase 1
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Masking Details
For groups 1, 2 and 3, participants and investigators will be blinded to group allocation. Study staff involved in storage and preparation of the vaccine will be aware of vaccine assignment but these staff will play no other role in the study. The Sponsor team will remain blinded with the exception of designated members of the laboratory team that will perform the final evaluation of the data. For groups 4 and 5, no study staff or participants will be blinded as the number of vaccinations in each group is different. For group 6, no study staff or participants will be blinded as the schedule of vaccinations in each group is different.
Purpose
PREVENTION
Intervention Model
PARALLEL
Model Details: RCT
Sponsor Type
OTHER
Responsible Party
SPONSOR

Study Record Dates

First Submitted

December 9, 2021

First Posted

December 13, 2021

Study Start

May 14, 2022

Primary Completion

May 27, 2025

Study Completion

May 27, 2025

Last Updated

April 8, 2026

Record last verified: 2025-09

Locations