Evaluation of Immunohistochemical Expression of Heparanase in Helicobacter Pylori-Associated Chronic Gastritis

NCT05073614 | Completed

• 1 other identifier: Soh-Med-21-09-43
• Study Type: interventional
• Target: 70
• Locations: 1 country
• Sites: 1

Brief Summary

Treatment of H.pylori may not be achieved in many patients with chronic gastritis. Termination of the inflammatory respose produced by h.pylori may be useful in management of difficult cases. Heparanase is a pro-inflammatory mediator. Blocking of heparanase may relief the symptoms of chronic gastritis.

Conditions

Chronic Gastritis

Keywords

Heparanase; Chronic Gastritis; Helicobacter Pylori

Outcome Measures

Primary Outcomes (1)

• Correlation of Heparanase expression in chronic gastritis.

  we will correlate the intensity and percentage of immunohistochemical expression of Heparanase to different parameters of chronic gastritis as severity of gastric atrophy, chronic inflammation, intensity of neutrophilic infiltrate and presence or absence of H. pylori.

  6 months

Study Arms (1)

patients with chronic gastritis

OTHER

patients with symptomatic chronic gastritis will undergo upper endoscopy, gastric biopsies will be obtained to establish giagnosis of chronic gastritis, detection of h.pylori and assessment of heparanase expression.

Genetic: immunohistochemical detection of Heparanase in chronic gastritis

Interventions

immunohistochemical detection of Heparanase in chronic gastritis GENETIC

immunohistochemical detection of Heparanase in chronic gastritis specimens.

patients with chronic gastritis

Eligibility Criteria

• Sex: all
• Healthy Volunteers: No
• Age Groups: Child (0-17), Adult (18-64), Older Adult (65+)

You may qualify if:

• patients with symptomatic chronic gastritis who underwent upper endoscopy.

You may not qualify if:

• patients with gastric carcinoma.

Sponsors & Collaborators

• Sohag University: lead

Study Sites (1)

Maisa Hashem Mohammed

Sohag, 82524, Egypt

Location

Study Design

• Study Type: interventional
• Phase: not applicable
• Allocation: NA
• Masking: NONE
• Purpose: BASIC SCIENCE
• Intervention Model: SINGLE GROUP
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: lecturer of pathology

Model Details: patients with chronic gastritis will undergo upper endoscopy, punch biopsies will be obtained and tissue sections will be prepared from each specimen. sections will be stained by H\&E to establish diagnosis of chronic gastritis. others will be stained by Giemsa staain to detect H.pylori, still others will be stained immunohistochemically by anti Heparanase antibody.

Study Record Dates

• First Submitted: September 19, 2021
• First Posted: October 11, 2021
• Study Start: July 1, 2021
• Primary Completion: December 30, 2021
• Study Completion: January 1, 2022
• Last Updated: February 4, 2022

Record last verified: 2022-01

Data Sharing

• IPD Sharing: Will not share

Locations

EG (1)