The Mechanism of Extracellular Vesicles Containing Mitochondrial DNA in ARDS Lung Injury Caused by Extrapulmonary Sepsis

NCT05061212 | Unknown

• 1 other identifier: ARDS-mtDNA EVs
• Study Type: observational
• Target: 50
• Locations: 0 countries
• Sites: N/A

Brief Summary

The acute respiratory distress syndrome (ARDS) remains a common and morbid complication of critical illness. Sepsis contribute to a lot of ARDS cases, but mechanisms by which non-pulmonary insults such as extrapulmonary sepsis propagate lung injury remain unclear. Most eukaryotic cells release small anuclear membrane-bound vesicles into the extracellular environment in either physiological or pathophysiological conditions, often called extracellular vesicles (EVs) .Through their cargo containing bioactive molecules such as proteins, mRNAs, and microRNAs and their interaction with target cells, EVs are recognized as important mediators of cellular communication. Mitochondrial contents are clearly present in EVs, and mitochondrial cargo within EVs have been shown to stimulate the production of proinflammatory cytokines, further enhancing LPS-induced inflammation. Among the mitochondrial contents, mtDNA was present at higher levels in EVs.Therefore, we hypothesis, EVs containing mtDNA play an important role in the occurrence and development of ARDS caused by extrapulmonary sepsis.

Conditions

ARDS, Human

Outcome Measures

Primary Outcomes (1)

• 28-day mortality

  All patients were followed up for 28 days

Secondary Outcomes (2)

• Ventilator days

  All patients were followed up for 28 days

• ICU stay

  All patients were followed up for 28 days

Eligibility Criteria

• Age: 18 Years+
• Sex: all
• Healthy Volunteers: No
• Age Groups: Adult (18-64), Older Adult (65+)
• Sampling Method: Probability Sample

Study Population

We enrolled patients with ARDS caused by abdominal infection

You may qualify if:

• Patients with ARDS caused by abdominal infection

You may not qualify if:

• age \<18 years old or pregnancy; death or discharge within 24 hours after admission; advanced tumor

Sponsors & Collaborators

• Southeast University, China: lead

MeSH Terms

Conditions

Respiratory Distress Syndrome

Condition Hierarchy (Ancestors)

Lung Diseases; Respiratory Tract Diseases; Respiration Disorders

Study Design

• Study Type: observational
• Observational Model: CASE ONLY
• Time Perspective: PROSPECTIVE
• Sponsor Type: OTHER
• Responsible Party: PRINCIPAL INVESTIGATOR
• PI Title: Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University

Study Record Dates

• First Submitted: September 18, 2021
• First Posted: September 29, 2021
• Study Start: October 1, 2021
• Primary Completion: September 1, 2022
• Study Completion: December 31, 2022
• Last Updated: September 29, 2021

Record last verified: 2021-09