NCT05048875

Brief Summary

The investigational study drug, JNJ-64281802, is being developed for the prevention and treatment of dengue infection. This study is hypothesizing that the highest dose of the investigational study drug is superior to receiving a placebo with respect to its antiviral activity in healthy adult participants inoculated with Dengue Serotype 3.

Trial Health

87
On Track

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Enrollment
56

participants targeted

Target at P25-P50 for phase_2

Timeline
Completed

Started Feb 2022

Geographic Reach
1 country

2 active sites

Status
completed

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

June 30, 2021

Completed
3 months until next milestone

First Posted

Study publicly available on registry

September 17, 2021

Completed
5 months until next milestone

Study Start

First participant enrolled

February 3, 2022

Completed
1.3 years until next milestone

Primary Completion

Last participant's last visit for primary outcome

May 16, 2023

Completed
1.3 years until next milestone

Study Completion

Last participant's last visit for all outcomes

September 10, 2024

Completed
8 months until next milestone

Results Posted

Study results publicly available

April 24, 2025

Completed
Last Updated

April 24, 2025

Status Verified

April 1, 2025

Enrollment Period

1.3 years

First QC Date

June 30, 2021

Results QC Date

May 16, 2024

Last Update Submit

April 4, 2025

Conditions

Keywords

Dengue Serotype 3Healthy Participants

Outcome Measures

Primary Outcomes (1)

  • Assess the Antiviral Activity of the Study Drug (JNJ 64281802) Versus Placebo in Terms of Reduction of Dengue Infection.

    Area under the DENV-3 RNA viral load (VL) concentration-time curves from immediately before inoculation (baseline on Day 1) until Day 29. Cohort 2 was recently unblinded (data analysis is in process) and Cohort 2 is not part of the primary outcome assessment. Therefore, it cannot yet be fully represented in the record (i.e., results).

    28 days

Secondary Outcomes (31)

  • Number of Adverse Events to Assess the Safety and Tolerability of the Study Drug (JNJ 64281802).

    85 Days

  • Physical Examinations to Assess the Safety and Tolerability of the Study Drug (JNJ 64281802).

    99 weeks

  • Recording of Vital Signs to Assess the Safety and Tolerability of the Study Drug (JNJ 64281802).

    99 weeks

  • 12-lead ECG With Measurement of QTcF to Assess the Safety and Tolerability of the Study Drug (JNJ 64281802).

    99 weeks

  • 12-lead ECG With Measurement of QRS Interval to Assess the Safety and Tolerability of the Study Drug (JNJ 64281802).

    99 weeks

  • +26 more secondary outcomes

Study Arms (4)

Cohort 1 - Group 1 JNJ High Dose

EXPERIMENTAL

Sentinel high-dose. Four participants will be enrolled in Group 1a before enrolling the remaining participants to Group 1b (12 participants total in Group 1, all same dosing regimen). Note group 1a and group 1b are combined into one arm, because the dosing regimen is the same and they were not separated during final statistical analysis.

Drug: Cohort 1 - Group 1 JNJ High Dose

Cohort 1 - Group 2 JNJ Medium Dose

EXPERIMENTAL

Group 2 includes Medium and low dose, 14 participants total. Enrolled after completion of Group 1a.

Drug: Cohort 1 - Group 2 JNJ Medium Dose

Cohort 1 - Group 2 JNJ Low Dose

EXPERIMENTAL

Group 2 includes Medium and low dose, 14 participants total. Enrolled after completion of Group 1a.

Drug: Cohort 1 - Group 2 JNJ Low Dose

Cohort 1 - Group 1/2 Placebo

PLACEBO COMPARATOR

Matching placebo. Note Group 1 placebo and group 2 placebo are combined per sponsor data analysis.

Drug: Cohort 1 - Group 1/2 Placebo

Interventions

High dose: 600-mg loading dose for 5 days/200-mg maintenance dose for 21 days

Also known as: JNJ-64281802 (Sentinel high dose)
Cohort 1 - Group 1 JNJ High Dose

Medium dose: 200-mg loading dose for 5 days/50-mg maintenance dose for 21 days

Also known as: JNJ-64281802 (medium dose)
Cohort 1 - Group 2 JNJ Medium Dose

Low dose: 40-mg loading dose for 5 days/10-mg maintenance dose for 21 days

Also known as: JNJ-64281802 (low dose)
Cohort 1 - Group 2 JNJ Low Dose

Matching placebo. Note Group 1 placebo and group 2 placebo are combined per sponsor data analysis.

Also known as: Placebo High Dose
Cohort 1 - Group 1/2 Placebo

Eligibility Criteria

Age18 Years - 55 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • Male or female.
  • to 55 years of age, inclusive, at time of screening.
  • Healthy on the basis of physical examination, medical history, and vital signs performed at screening.
  • Healthy on the basis of clinical laboratory tests performed at screening.
  • Must pass the comprehension assessment indicating that the participant understands the purpose, procedures, and potential risks and benefits of the study, after reading the informed consent and after the investigator or designee has provided detailed information on the study and answered the potential participant's questions.
  • Must have a body mass index between 18.0 and 35.0 kg/m2, inclusive.
  • Must have a normal electrocardiogram (ECG, test which displays a person's heartbeat) at screening.
  • Must have a blood pressure (after lying face up for greater than or equal to 5 minutes) between 90 and 140 mmHg systolic and less than or equal to 90 mmHg diastolic at screening.
  • Must complete the informed consent process independently and without assistance and sign an informed consent form (ICF) indicating that he or she understands the purpose of, and procedures required for, the study and is willing to participate in the study.
  • All persons of childbearing potential must have a negative pregnancy test at screening.
  • A volunteer must be:
  • Not of childbearing potential, or
  • Of childbearing potential and practicing a highly effective, preferably user independent method of contraception and agrees to remain on a highly effective method while receiving study drug and until 90 days after the last dose of study drug.
  • A person of childbearing potential must agree not to donate eggs for the purposes of assisted reproduction during the study and for 90 days after the last dose of study drug.
  • During the study and for 90 days after the last dose of study drug, persons who are having sexual relationships in which their partner may become pregnant must wear a condom when engaging in any activity that allows for passage of ejaculate to another person. Persons who are having sexual relationships in which their partner may become pregnant should also be advised of the benefit for their partner to use a highly effective method of contraception as condoms may break or leak.
  • +14 more criteria

You may not qualify if:

  • History of liver or renal impairment; significant cardiac, vascular, pulmonary, gastrointestinal (such as significant diarrhea, constipation lasting greater than 2 days), endocrine, neurologic, hematologic, rheumatologic, neoplastic, autoimmune, or metabolic disturbances.
  • Known allergies, hypersensitivity, or intolerance to the study drug (JNJ-64281802) or its inactive substances, or an acute, life threatening allergic reaction or swelling following study drug administration.
  • History of a severe allergic reaction or anaphylaxis (which is a severe, potentially life-threatening allergic reaction).
  • Taken any substances or therapies that are not allowed before the first dose of study drug.
  • Received an investigational intervention or participated in another investigational clinical trial (including investigational vaccines) within 6 months before first dose of study drug, or is currently enrolled in an investigational study, or is planning to be enrolled in an investigational study within 90 days after last dose of study drug. With the exception of participation in COVID-19 vaccine trials and receipt COVID-19 vaccines licensed or under Emergency Use Authorization which can be received at any time.
  • Persons of childbearing potential only: Pregnant as determined by a positive pregnancy blood test, breastfeeding, or planning to become pregnant during the study or within 90 days after last dose of study drug.
  • Plans to impregnate and help conceive a child during the study or within 90 days after last dose of study drug.
  • Any condition for which, in the opinion of the study doctor, participation would not be in the best interest of the participant.
  • Blood test confirming current infection with human immunodeficiency virus type 1 or 2 (HIV-1 or HIV-2), hepatitis B virus (HBV), or hepatitis C virus (HCV), or blood test confirming past or current infection with any of the following flaviviruses: dengue, Zika virus (ZIKV), West Nile virus, or St. Louis Encephalitis (SLE) virus or vaccination for dengue, Zika virus, or Japanese Encephalitis virus (JEV) Note: Blood laboratory testing will assess the presence of antibodies at screening.
  • Recent (in the past 4 weeks) travel to any dengue-endemic region (as defined by the US CDC) or having definite plans to travel to a dengue endemic region, during the study. Potential participants may be eligible for enrollment greater than or equal to 4 weeks after their return from a dengue-endemic region.
  • Received or plans to receive:
  • Licensed live attenuated vaccines - within 28 days before first dose of study drug until 28 days after last dose of study drug.
  • Other licensed (not live) vaccines - within 14 days before first dose of study drug until 14 days after last dose of study drug.
  • COVID-19 vaccines, either licensed or under EUA, are allowed at any time during the study however every effort will be made to avoid the above windows of time of administration.
  • Note: Vaccinations against DENV and Zika virus are not allowed until 90 days after last dose of study drug.
  • +16 more criteria

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Study Sites (2)

Johns Hopkins University, Bloomberg School of Public Health

Baltimore, Maryland, 21202, United States

Location

University of Vermont Medical Center (UVMMC), Clinical Research Center

Burlington, Vermont, 05401, United States

Location

Related Publications (1)

  • Durbin AP, Van Wesenbeeck L, Pierce KK, Herrera-Taracena G, Ebone L, Buelens A, Lutton P, Sabundayo BP, Van Eygen V, De Clerck K, Fetter I, Voge NV, Fang X, Goeyvaerts N, Vandendijck Y, Mayfield J, Lenz O, De Meyer S, Kakuda TN, He H, Amaro-Carambot E, Akli RD, Carmolli M, De Marez T, Whitehead SS, Van Loock M, Rasschaert F. Daily Mosnodenvir as Dengue Prophylaxis in a Controlled Human Infection Model. N Engl J Med. 2025 Nov 27;393(21):2107-2118. doi: 10.1056/NEJMoa2500179.

MeSH Terms

Conditions

Dengue

Condition Hierarchy (Ancestors)

Mosquito-Borne DiseasesVector Borne DiseasesInfectionsArbovirus InfectionsVirus DiseasesFlavivirus InfectionsFlaviviridae InfectionsRNA Virus InfectionsHemorrhagic Fevers, Viral

Results Point of Contact

Title
Dr Anna Durbin
Organization
Johns Hopkins School of Public Health Center for Immunization Research

Study Officials

  • Anna Durbin, MD

    Center for Immunization Research, Johns Hopkins School of Public Health

    PRINCIPAL INVESTIGATOR

Publication Agreements

PI is Sponsor Employee
No
Restrictive Agreement
No

Study Design

Study Type
interventional
Phase
phase 2
Allocation
RANDOMIZED
Masking
TRIPLE
Who Masked
PARTICIPANT, CARE PROVIDER, INVESTIGATOR
Masking Details
The subject, investigator, and clinical staff will not know which treatment group the subject has been assigned. In addition, other personnel assigned to monitor the study will not know the treatment assignment of the subject. The pharmacist will be unblinded as the study drug will be provided as bulk supplies.
Purpose
PREVENTION
Intervention Model
SEQUENTIAL
Model Details: There are two cohorts and three groups in each cohort. Cohort 1 consists of Group 1a, Group 1b and Group 2. Cohort 2 consists of Group 3, Group 4, and Group 5. Within each group, participants will either be randomized to receive study drug or placebo. Four participants were enrolled in Group 1a before enrolling the remaining participants to Group 1b (12 participants) and Group 2 (14 participants) in Cohort 1. Based on the interim analysis results of Cohort 1, three groups for Cohort 2 were created. Results are posted for Cohort 1 which is complete and unblinded. Cohort 2 has been fully enrolled and is in progress.
Sponsor Type
NIH
Responsible Party
SPONSOR

Study Record Dates

First Submitted

June 30, 2021

First Posted

September 17, 2021

Study Start

February 3, 2022

Primary Completion

May 16, 2023

Study Completion

September 10, 2024

Last Updated

April 24, 2025

Results First Posted

April 24, 2025

Record last verified: 2025-04

Data Sharing

IPD Sharing
Will not share

Locations