NCT05024188

Brief Summary

The intestinal microbiome is a microbial system that is influenced by host genetics and environmental exposures such as nutrition, stress and medications. There is a growing body of evidence indicating the significant contribution of the gut microbiome to host health and disease. Furthermore, it has been shown that exercise may modify the microbiome composition. However, important mechanistic questions related to the possible associations between exercise and the human gut microbiome remain unanswered. In this study, the investigators are using advanced state-of-the-art measurements of physical activity level and related metabolic parameters whether there is a connection between the microbiome and physical abilities in healthy participants and whether antibiotics consumption can influence host physical abilities and glycemic responses through changes induced in microbiome composition and function.

Trial Health

35
At Risk

Trial Health Score

Automated assessment based on enrollment pace, timeline, and geographic reach

Trial has exceeded expected completion date
Enrollment
50

participants targeted

Target at P25-P50 for not_applicable

Timeline
Completed

Started Jan 2022

Status
unknown

Health score is calculated from publicly available data and should be used for screening purposes only.

Trial Relationships

Click on a node to explore related trials.

Study Timeline

Key milestones and dates

First Submitted

Initial submission to the registry

August 4, 2021

Completed
23 days until next milestone

First Posted

Study publicly available on registry

August 27, 2021

Completed
5 months until next milestone

Study Start

First participant enrolled

January 20, 2022

Completed
6 months until next milestone

Primary Completion

Last participant's last visit for primary outcome

July 20, 2022

Completed
6 months until next milestone

Study Completion

Last participant's last visit for all outcomes

January 20, 2023

Completed
Last Updated

December 28, 2021

Status Verified

December 1, 2021

Enrollment Period

6 months

First QC Date

August 4, 2021

Last Update Submit

December 27, 2021

Conditions

Outcome Measures

Primary Outcomes (3)

  • Microbiome Profiling

    Collection of stool and buccal samples samples for microbiome composition analysis with16s rRNA sequencing.

    2 years

  • Body Mass Index (BMI)

    The BMI is defined as the body mass divided by the square of the body height, and is expressed in units of kg/m\^2

    2 years

  • Body Circumferences

    waist, hips, and limbs (cm).

    2 years

Secondary Outcomes (2)

  • Glycemic Responses

    2 years

  • Bone Mineral Density (BMD)

    2 years

Study Arms (2)

Antibiotics + FMT

EXPERIMENTAL

7 days of antibiotics- Ciprofloxacin, 500 mg 2/day \& Metronidazole (Flagyl), 500 mg 3/day. After antibiotics administration, participants will receive 10 aFMT capsules for three consecutive days (a total of 30 capsules).

Drug: AntibioticsOther: Fecal Microbiota Transplantation

Placebo

PLACEBO COMPARATOR

7 days of cellules pills. After cellules pills administration, participants will receive 10 agarose capsules for three consecutive days (a total of 30 capsules).

Other: Cellules PillsOther: Agarose Capsules

Interventions

Ciprofloxacin, 500 mg 2/day \& Metronidazole (Flagyl), 500 mg 3/day.

Also known as: Ciprofloxacin & Metronidazole
Antibiotics + FMT

Fecal Microbiota Transplantation is the process of transferring stool from a healthy donor to another.

Also known as: FMT
Antibiotics + FMT

7 days of cellules placebo pills.

Placebo

Placebo capsules consist a combination of agarose in normal saline/glycerol (the same vehicle as in a FMT capsules)

Placebo

Eligibility Criteria

Age20 Years - 35 Years
Sexall
Healthy VolunteersYes
Age GroupsAdult (18-64)

You may qualify if:

  • BMI\<28
  • Age - 20-35
  • Are used to exercise in aerobic sports (running, swimming, Zumba, ball games, functional training, surfing, tennis, wrestling ext.).
  • Exercise at least twice a week, for a minimum duration of the last 6 months.

You may not qualify if:

  • Consumption of antibiotics (PO) or probiotics 3 months prior to the first day of the experiment.
  • Diagnosis of type 1 or type 2 diabetes.
  • Pregnancy, fertility treatments, breastfeeding women six months prior to enrollment and during the study.
  • Chronic disease (e.g. AIDS, Cushing syndrome, CKD, acromegaly, hyperthyroidism etc.)
  • Cancer and recent anticancer treatment
  • Psychiatric disorders
  • Coagulation disorders
  • Gastrointestinal disorders
  • Bariatric surgery
  • Alcohol or substance abuse
  • BMI\>28
  • Any physical condition precluding the completion of a routine effort test

Contact the study team to confirm eligibility.

Sponsors & Collaborators

Related Publications (2)

  • Clarke SF, Murphy EF, O'Sullivan O, Lucey AJ, Humphreys M, Hogan A, Hayes P, O'Reilly M, Jeffery IB, Wood-Martin R, Kerins DM, Quigley E, Ross RP, O'Toole PW, Molloy MG, Falvey E, Shanahan F, Cotter PD. Exercise and associated dietary extremes impact on gut microbial diversity. Gut. 2014 Dec;63(12):1913-20. doi: 10.1136/gutjnl-2013-306541. Epub 2014 Jun 9.

    PMID: 25021423BACKGROUND
  • Barton W, Penney NC, Cronin O, Garcia-Perez I, Molloy MG, Holmes E, Shanahan F, Cotter PD, O'Sullivan O. The microbiome of professional athletes differs from that of more sedentary subjects in composition and particularly at the functional metabolic level. Gut. 2018 Apr;67(4):625-633. doi: 10.1136/gutjnl-2016-313627. Epub 2017 Mar 30.

    PMID: 28360096BACKGROUND

MeSH Terms

Interventions

Anti-Bacterial AgentsCiprofloxacinMetronidazoleFecal Microbiota TransplantationSepharose

Intervention Hierarchy (Ancestors)

Anti-Infective AgentsTherapeutic UsesPharmacologic ActionsChemical Actions and UsesFluoroquinolones4-QuinolonesQuinolonesQuinolinesHeterocyclic Compounds, 2-RingHeterocyclic Compounds, Fused-RingHeterocyclic CompoundsNitroimidazolesNitro CompoundsOrganic ChemicalsImidazolesAzolesHeterocyclic Compounds, 1-RingBiological TherapyTherapeuticsPolysaccharidesCarbohydrates

Study Officials

  • Ilan Youngster, Dr.

    Assaf-Harofeh MC, Bee'r Yaakov Israel

    PRINCIPAL INVESTIGATOR

Central Study Contacts

Study Design

Study Type
interventional
Phase
not applicable
Allocation
RANDOMIZED
Masking
DOUBLE
Who Masked
PARTICIPANT, INVESTIGATOR
Purpose
BASIC SCIENCE
Intervention Model
PARALLEL
Sponsor Type
OTHER GOV
Responsible Party
SPONSOR INVESTIGATOR
PI Title
Principal Investigator

Study Record Dates

First Submitted

August 4, 2021

First Posted

August 27, 2021

Study Start

January 20, 2022

Primary Completion

July 20, 2022

Study Completion

January 20, 2023

Last Updated

December 28, 2021

Record last verified: 2021-12

Data Sharing

IPD Sharing
Will not share